Tumor Deposit Is an Independent Factor Predicting Early Recurrence and Poor Prognosis in Gastric Cancer

Fujikawa, Kaoru; Omori, Takeshi; Shinno, Naoki; Hara, Hisashi; Yamamoto, Masaaki; Yasui, Masayoshi; Matsuda, Chu; Wada, Hiroshi; Nishimura, Junichi; Haraguchi, Naotsugu; Akita, Hirofumi; Ohue, Masayuki; Miyata, Hiroshi

doi:10.1007/s11605-023-05668-y

Cited by 5 publications

(1 citation statement)

References 24 publications

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“…The main known risk factors for GC recurrence and metastasis are still related to the tumor stage, the extension of the surgery, the lymph node affectation, the tumor size and other non-molecular aspects (12)(13)(14), remaining the molecular determinants of GC progression unknown. In this scenario, we used the publicly available dataset of the GC patients from the TCGA cohort and identified the genes most significantly associated with GC prognosis.…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel gene signature related to prognosis and metastasis in gastric cancer

Matheu,

Elizazu,

Otaegi-Ugartemendia

et al. 2023

Preprint

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Background: Gastric Cancer (GC) presents poor outcome, which is consequence of the high incidence of recurrence and metastasis at early stages. GC patients presenting recurrent or metastatic disease display a median life expectancy of only 8 months. The mechanisms underlying GC progression remain poorly understood. Methods: We took advantage of public available GC datasets from TCGA using GEPIA, and identified the matched genes among the 100 genes most significantly associated with overall survival (OS) and disease free survival (DFS). Results were confirmed in ACRG cohort and in over 2,000 GC cases obtained from several cohorts integrated using our own analysis pipeline. The Kaplan-Meier method and multivariate Cox regression analyses were used for prognostic significance and linear modelling and correlation analyses for association with clinic-pathological parameters and biological hallmarks. In vitro and in vivo functional studies were performed in GC cells with candidate genes and the related molecular pathways were studied by RNA sequencing. Results: High expression of ANKRD6, ITIH3, SORCS3, NPY1R and CCDC178 individually and as a signature was associated with poor prognosis and recurrent disease in GC. Among the 5 genes, ANKRD6, ITIH3 and NPY1R represented independent prognostic factors determined through multivariate Cox regression analyses. Moreover, the expression of ANKRD6 and ITIH3 was significantly higher in metastasis and its levels associated to EMT and stemness markers. Moreover, ANKRD6 silencing in GC metastatic cells showed impairment in GC tumorigenic activity and EMT in functional studies in vivo and in molecular pathways in transcriptomic studies. Conclusions: Our study identified a novel signature involved in GC malignancy and prognosis, and revealed a novel pro-metastatic role of ANKRD6 in GC.

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Section: Introductionmentioning

confidence: 99%

Identification of a novel gene signature related to prognosis and metastasis in gastric cancer

Matheu,

Elizazu,

Otaegi-Ugartemendia

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Identification of a novel gene signature related to prognosis and metastasis in gastric cancer

Elizazu,

Artetxe-Zurutuza,

Otaegi-Ugartemendia

et al. 2024

Cell Oncol.

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Background Gastric Cancer (GC) presents poor outcome, which is consequence of the high incidence of recurrence and metastasis at early stages. GC patients presenting recurrent or metastatic disease display a median life expectancy of only 8 months. The mechanisms underlying GC progression remain poorly understood. Methods We took advantage of public available GC datasets from TCGA using GEPIA, and identified the matched genes among the 100 genes most significantly associated with overall survival (OS) and disease free survival (DFS). Results were confirmed in ACRG cohort and in over 2000 GC cases obtained from several cohorts integrated using our own analysis pipeline. The Kaplan-Meier method and multivariate Cox regression analyses were used for prognostic significance and linear modelling and correlation analyses for association with clinic-pathological parameters and biological hallmarks. In vitro and in vivo functional studies were performed in GC cells with candidate genes and the related molecular pathways were studied by RNA sequencing. Results High expression of ANKRD6, ITIH3, SORCS3, NPY1R and CCDC178 individually and as a signature was associated with poor prognosis and recurrent disease in GC. Moreover, the expression of ANKRD6 and ITIH3 was significantly higher in metastasis and their levels associated to Epithelial to Mesenchymal Transition (EMT) and stemness markers. In line with this, RNAseq analysis revealed genes involved in EMT differentially expressed in ANKRD6 silencing cells. Finally, ANKRD6 silencing in GC metastatic cells showed impairment in GC tumorigenic and metastatic traits in vitro and in vivo. Conclusions Our study identified a novel signature involved in GC malignancy and prognosis, and revealed a novel pro-metastatic role of ANKRD6 in GC.

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Prognostic effect of categorized tumor deposits in gastric cancer: A single-center retrospective study

Hayashi,

Abe,

Fujita

et al. 2024

Surgery

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Tumor Deposit Is an Independent Factor Predicting Early Recurrence and Poor Prognosis in Gastric Cancer

Cited by 5 publications

References 24 publications

Identification of a novel gene signature related to prognosis and metastasis in gastric cancer

Identification of a novel gene signature related to prognosis and metastasis in gastric cancer

Identification of a novel gene signature related to prognosis and metastasis in gastric cancer

Prognostic effect of categorized tumor deposits in gastric cancer: A single-center retrospective study

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