Tumor‐derived exosomes influence the cell cycle and cell migration of human esophageal cancer cell lines

Matsumoto, Yasunori; Kano, Masayuki; Murakami, Kentaro; Toyozumi, Takeshi; Suito, Hiroshi; Takahashi, Masahīko; Sekino, Nobufumi; Shiraishi, Taizo; Kamata, Tomoko; Ryuzaki, Takahiro; Hirasawa, Soichiro; Kinoshita, Kazuya; Matsubara, Hisahiro

doi:10.1111/cas.14660

Cited by 40 publications

(22 citation statements)

References 25 publications

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“…MiR-224-5p can be transferred from SW620 cells to CCD 841 CoN cells via exosomes Evidences have shown that cancer cell-derived exosomes exert a vital role in the process of malignant transformation [23,24]. In this study, exosomes were isolated from the CM of CCD 841 CoN cells and SW620 cells.…”

Section: Cmtm4 Is a Binding Target Of Mir-224-5pmentioning

confidence: 98%

Exosomal miR-224-5p from colorectal cancer cells promotes malignant transformation of human colon normal cells by promoting cell proliferation through downregulation of CMTM4

Yang

Guo-yin

et al. 2021

Preprint

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Background Interactions between malignant cells and neighboring normal cells is important for carcinogenesis. In addition, cancer cell-derived exosomes have been shown to promote the malignant transformation of recipient cells, but the mechanisms remain unclear. Methods The level of miR-224-5p in colorectal cancer (CRC) cell-derived exosomes was determined by RT-qPCR assay. In addition, PKH26 dye-labeled exosomes were used to assess the efficacy of the transfer of exosomes between SW620 and CCD 841 CoN cells. Results In this study, we found that overexpression of miR-224-5p significantly promoted the proliferation, migration and invasion of SW620 cells. In addition, miR-224-5p can be transferred from SW620 cells to CCD 841 CoN cells via exosomes. SW620 cell-derived exosomes overexpressing miR-224-5p markedly promoted proliferation, migration and invasion of CCD 841 CoN cells. Meanwhile, SW620 cell-derived exosomal miR-224-5p notably decreased the expression of CMTM4 in CCD 841 CoN cells. Furthermore, SW620 cell-derived exosomal miR-224-5p significantly inhibited tumor growth in a xenograft model in vivo. Conclusion These findings suggested that SW620 cell-derived exosomal miR-224-5p could promote malignant transformation and tumorigenesis in vitro and in vivo via downregulation of CMTM4, suggesting that miR-224-5p might be a potential target for therapies in CRC.

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Section: Cmtm4 Is a Binding Target Of Mir-224-5pmentioning

confidence: 98%

Exosomal miR-224-5p from colorectal cancer cells promotes malignant transformation of human colon normal cells by promoting cell proliferation through downregulation of CMTM4

Yang

Guo-yin

et al. 2021

Preprint

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“… 19 Matsumoto et al demonstrated that tumor‐derived exosomes could promote tumor progression and malignant transformation by altering gene expression and phenotype of tumor cells. 20 ESCC cell-derived exosomes containing lncRNA prostate cancer-associated transcript 1 (PCAT1) promoted ESCC cell proliferation by sponging tumor suppressor miR-326. 21 Knockdown of PCAT1 could induce cell cycle arrest at G2/M phase, and increased paclitaxel sensitivity.…”

Section: Exosomes Regulate the Growth And Metastasis Of Ecmentioning

confidence: 99%

The Significance of Exosomes in Pathogenesis, Diagnosis, and Treatment of Esophageal Cancer

Zhao

Chen

Gong

2021

IJN

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Esophageal cancer is one of the most common malignancy in China with high mortality. Understanding pathogenesis and identifying early diagnosis biomarkers can significantly improve the prognosis of patients with esophageal cancer. Exosomes are small vesicular structures containing a variety of components (including DNA, RNA, and proteins) mediating cell-to-cell material exchange and signal communication. Growing evidences have shown that exosomes and its components are involved in growth, metastasis and angiogenesis in cancer, and could also be used as diagnostic and prognostic markers. In this review, we summarized recent progress to elucidate the significance of exosomes in the esophageal cancer progression, microenvironment remodeling, therapeutic resistance, and immunosuppression. We also discuss the utility of exosomes as diagnostic and prognostic biomarkers and therapeutic tool in esophageal cancer.

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“…We further confirmed that these vesicles were EVs by Western blot analysis of the tetraspanin molecules CD63 and TSG101 and the non-EVs protein GM-130. We also utilized a transmission electron microscope, HT7800 (Hitachi, Tokyo, Japan) to observe EVs according to the manufacturer's instructions [44].…”

Section: Cell Evs Isolation and Identificationmentioning

confidence: 99%

Esophageal Cancer-Derived Extracellular Vesicle miR-21-5p Contributes to EMT of ESCC Cells by Disorganizing Macrophage Polarization

Song

Yang

et al. 2021

Cancers

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The disorganized polarization of tumor-associated macrophages (TAMs) exerts a critical effect on tumor progression. MicroRNAs (miRNAs) in extracellular vesicles (EVs) secreted from cancer cells may contribute to this process. However, the relationship between TAMs and EVs-miRNAs-mediated regulation in esophageal squamous cell carcinoma (ESCC) remains unclear. In the present study, immunoaffinity magnetic beads combined with antiepithelial cell adhesion molecules (EpCAM) were used to isolate and identify EVs-miR-21-5p from the plasma of ESCC patients. An in vitro coculture system was designed to evaluate the effect of esophageal cancer cells with miR-21-5p overexpression on macrophage polarization. We found that phorbol myristate acetate-induced THP-1 macrophages took up EVs-miR-21-5p from EC109 or EC9706 cells and were transformed into M2 macrophages. This, in turn, contributed to the excessive migration and invasion of esophageal cancer cells. The mechanism underlying these changes may involve activation of M2 macrophages by upregulated ESCC-derived EVs-miR-21-5p through the PTEN/AKT/STAT6 pathway. This may result in esophageal cancer cell epithelial-mesenchymal transition (EMT) via TGF-β/Smad2 signaling. Our results indicate positive feedback between M2 macrophage polarization and EMT of esophageal cancer cells in the tumor microenvironment via shuttling of miR-21-5p in tumor-derived EVs.

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Tumor‐derived exosomes influence the cell cycle and cell migration of human esophageal cancer cell lines

Cited by 40 publications

References 25 publications

Exosomal miR-224-5p from colorectal cancer cells promotes malignant transformation of human colon normal cells by promoting cell proliferation through downregulation of CMTM4

Exosomal miR-224-5p from colorectal cancer cells promotes malignant transformation of human colon normal cells by promoting cell proliferation through downregulation of CMTM4

The Significance of Exosomes in Pathogenesis, Diagnosis, and Treatment of Esophageal Cancer

Esophageal Cancer-Derived Extracellular Vesicle miR-21-5p Contributes to EMT of ESCC Cells by Disorganizing Macrophage Polarization

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