Tumor formation of prostate cancer cells influenced by stromal cells from the transitional or peripheral zones of the normal prostate

Zhao, Fu-Jun; Han, Bangmin; Yu, Shengqiang; Xia, Shujie

doi:10.1038/aja.2008.33

Cited by 19 publications

(27 citation statements)

References 17 publications

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“…Based on our data the zonal origin of the inductive fibroblast is of less importance. Zhao et al showed that TZ fibroblasts inhibited the growth of androgen-independent PC-3 cells in vivo [17]. …”

Section: Discussionmentioning

confidence: 99%

Human Prostate Fibroblasts Induce Growth and Confer Castration Resistance and Metastatic Potential in LNCaP Cells

et al. 2010

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Background-The tumor microenvironment is important for progressive and metastatic disease.Objective-To study the hypothesis that prostate fibroblasts have differential ability to induce castration-resistant prostate cancer (PCa) and metastatic progression and whether this effect might vary depending on the zonal origin of the fibroblast.Design, setting, and participants-Human prostate fibroblasts from the peripheral (PZ), transition (TZ) and central (CZ) zones of radical prostatectomy specimens (n = 13) were isolated and compared for their ability to promote androgen independence and metastatic progression in androgen-responsive PCa lymph node carcinoma of the prostate (LNCaP) cells in vivo. Interventions-By Take-home messageThe tumor microenvironment is important for disease progression and development of metastatic disease. Prostate fibroblasts can induce prostate cancer cell growth and confer castration resistance and the development of a metastatic phenotype. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript P4-2 (derivatives from interaction with PZ), T4, and T4-2 (derivatives from interaction with TZ)-were established and characterized.Measurements-In vivo and in vitro evaluation of induction of tumor growth and metastatic potential.Results and limitations-1) LNCaP sublines were permanently altered in their cytogenetic and biologic profiles after cellular interaction with prostate stromal fibroblasts. LNCaP sublines grew faster under anchorage-dependent and -independent conditions, expressed 1-12-fold more prostatespecific antigen in vitro than LNCaP cells, and gained metastatic potential; 2) zonal differences of stromal fibroblasts in their ability to induce the growth and progression of LNCaP tumors as xenografts in mice may exist but need further analysis; 3) PZ-conditioned medium induced more anchorage-independent growth of LNCaP cells in vitro. TZ had a higher growth rate and were more sensitive to dihydrotestosterone.Conclusions-We demonstrate that prostate fibroblasts have growth inductive potential on PCa cells and affect their subsequent progression to castration resistance and development of a metastatic phenotype.

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Section: Discussionmentioning

confidence: 99%

Human Prostate Fibroblasts Induce Growth and Confer Castration Resistance and Metastatic Potential in LNCaP Cells

et al. 2010

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“…Stromal cells were separated from epithelial cells by discontinuous gradient centrifugation. 23 The stromal cells derived from the PZ (PZsc) and stromal cells derived from the TZ (TZsc) were identified by immunocytochemistry (IHC) 8 and cultured with RPMI 1640 supplemented with 10% foetal bovine serum (Gibco, Melbourne, Australia) and antibiotics (100 mg ml 21 streptomycin and 100 IU ml 21 penicillin) (Gibco BRL, Grand Island, NY, USA) at 37 uC under 5% CO 2 and a humidified atmosphere. The stromal cells were used at passages 3-5.…”

Section: Cell Culturementioning

confidence: 99%

“…Several studies have shown that the expression of certain genes differs between these two zones. [5][6][7][8][9] Feneley et al 10 reported that the epithelial content was significantly greater in the TZ than in the PZ. Conversely, concentrations of androgen receptor (AR) activity were higher in the PZ epithelium than in the TZ epithelium, but higher concentrations of oestrogen receptor activity were detected in the TZ zone stroma.…”

Section: Introductionmentioning

confidence: 99%

The differential effects of prostate stromal cells derived from different zones on prostate cancer epithelial cells under the action of sex hormones

Jiang

Han²,

Zhao³

et al. 2011

Asian J Androl

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It is well known that prostate cancer (PCa) occurs predominantly in the peripheral zone (PZ), whereas benign prostatic hyperplasia (BPH) typically develops in the transition zone. To identify possible mechanisms underlying zonal differences, we compared the effects of prostate stromal cells derived from the peripheral zone (PZsc) and the transition zone (TZsc) on a PCa epithelial cell line (PC3) in the presence of sex hormones. First, we observed that androgen receptor (AR) mRNA was more highly expressed in PZsc than TZsc when the cells were treated with dihydrotestosterone (DHT) and b-oestradiol (E2) (P,0.05). By ELISA, we looked for differences in the secretion of peptide growth factors from PZsc and TZsc. We found that keratinocyte growth factor (KGF) secretion increased with increasing concentrations of DHT (P,0.01) and was higher in PZsc than TZsc. Under treatment with DHT plus E2, PZsc secreted more transforming growth factor-b1 (TGF-b1) than TZsc, but this pattern was reversed when the cells were treated with E2 only. With increasing concentrations of DHT, insulin-like growth factor-1 (IGF-1) secretion increased in PZsc but decreased in TZsc. To further characterize the effects of PZsc and TZsc on PC3 cells, we developed a coculture model and performed MTT assays, Western blot analysis and real-time RT-PCR. We found that PZsc promoted PC3 cell proliferation and progression better than TZsc, particularly when treated with 10 nmol l 21 DHT plus 10 nmol l 21 E2. In conclusion, our data suggest that PZsc may have a greater capacity to induce PCa development and progression than TZsc via growth factors regulated by sex hormones. These findings provide possible mechanisms underlying zonal differences in prostate diseases, which may aid the search for novel therapeutic targets for PCa.

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“…25 % of prostate cancer may arise from the central zone. Zhao F.J. et al, 7 noted in their study that there are morphological and ultra-structural differences in stromal cells from transitional zone (TZ) and peripheral zone (PZ) of the normal prostate. In all, 514 differentially expressed genes were selected by microarray analysis; 483 genes were more highly expressed in stromal cells IJBAR (2013) Most common metastasis associated with prostate cancer is skeletal metastasis spread through venous route.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic Analysis of Prostate Carcinoma Patients

Chauhan

Damor

Solanki

et al. 2013

Int J of Biomed & Adv Res

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Background & objectives:

Prostate carcinoma is the second leading cause of cancer death in men above age of 60 years worldwide. More than 80% of the cancers concomitantly arise with Benign prostate hyperplasia. Prostate carcinoma is associated with various lower urinary tract symptoms, which affect their day to day life. The aim of the present investigation was to find out the major chromosomal aberrations present in Prostate carcinoma patients and to make a comparison with other study.

Methods:

In present study 05 cases of prostate carcinoma were taken on the basis of clinical diagnosis from the Gujarat Cancer Research Institute (GCRI), Ahmedabad, Gujarat, India, during the period of March 2011 to October 2011. The patients were analyzed for chromosomal aberrations using cultured peripheral blood lymphocytes with their pre-informed written consent.

Results:

In the present study, all 5 cases were > 60 years of age. 3(60%) cases of prostate carcinoma have elevated PSA level (>4 ng/ml). In adenocarcinoma of prostate gain, deletion, and translocation of 7q22-q31 is common. In present study, Karyotype analysis reveal 01 prostate carcinoma patient had 46,XY,del(7)(q31). In present study 2 cases shows evidence of metastasis.

Conclusion: Prostate carcinoma is found in > 60 years of age. Chromosome 1, 2, 3, 6, 7, 9, 16, 22, X and many more chromosomes were affected in prostate carcinoma patients, Common chromosomal aberration is 46,XY,del(7)(q31). Patients with prostate carcinoma have elevated PSA level (>4 ng/ml). Prostate carcinoma associated with metastasis have poor prognosis.

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Tumor formation of prostate cancer cells influenced by stromal cells from the transitional or peripheral zones of the normal prostate

Cited by 19 publications

References 17 publications

Human Prostate Fibroblasts Induce Growth and Confer Castration Resistance and Metastatic Potential in LNCaP Cells

Human Prostate Fibroblasts Induce Growth and Confer Castration Resistance and Metastatic Potential in LNCaP Cells

The differential effects of prostate stromal cells derived from different zones on prostate cancer epithelial cells under the action of sex hormones

Cytogenetic Analysis of Prostate Carcinoma Patients

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