Tumor inhibitors. 125. Isolation of potent new antileukemic trichothecenes from Baccharis megapotamica

Sm, Kupchan; Dr, Streelman; Bb, Jarvis; Rg, Dailey; At, Sneden

doi:10.1021/jo00862a011

Cited by 78 publications

(37 citation statements)

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“…Tricotecenos macrocíclicos similares, extraídos de outra espécie de Baccharis (B. megapotamica var. weirii), foram capazes de destruir células neoplásicas em linfomas de células B em ratos em uma investigação sobre plantas com atividade antiblástica (Kupchan et al 1977); isso pode explicar a necrose linfóide encontrada em alguns dos animais afetados pela intoxicação. A reprodução experimental (Tokarnia et al 1992) e a observação de surtos espontâneos (Driemeier et al 1999) da intoxicação por B. megapotamica já foi descrita em bovinos.…”

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Intoxicação espontânea por Baccharis coridifolia em bovinos

et al. 2005

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Intoxicação espontânea por Baccharis coridifolia em bovinos

et al. 2005

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“…1998, Varaschin & Alessi 2003. B. megapotamica (Kupchan et al 1977) B. coridifolia, (Busam & Habermehl 1982, Habermehl et al1985 and B. artemisioides (Rizzo et al 1997) contain a series of potent cytotoxic agents belonging to the highly cytotoxic macrocyclic trichothecene complex previously believed to be produced only by fungi (Jarvis et al 1996). In the case of B. megapotamica, the macrocyclic trichothecenes accumulate in the plant as baccharinoids ( B1, B2, B3, B4 etc.…”

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Experimental poisoning by Baccharis megapotamica var. weirii in buffalo

et al. 2012

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Pesq. Vet. Bras. 32(5):383-390, maio 2012 383 RESUMO.-[Intoxicação experimental por Baccharis megapotamica var. weirii em búfalos.] As partes aéreas verdes de Baccharis megapotamica var. weirii foram administradas oralmente a cinco búfalos da raça Murrah de 6-8 meses de idade nas doses de 1, 3, 4, 5 e 10g/kg de peso corporal (pc) (~1-10mg de tricotecenos macrocíclicos/kg/ pc). A planta usada no experimento foi colhida numa fazenda onde um surto recente de intoxicação espontânea por essa planta havia ocorrido. Nos búfalos deste experimento, os sinais clínicos apareceram 4-20 horas e 4 búfalos morreram 18-49 horas após a ingestão da planta. Os sinais clíni-cos consistiram de apatia, anorexia, diarreia aquosa, febre, cólica, salivação, tremores musculares, inquietação, respiração laboriosa, atonia ruminal e desidratação. Five male 6-8 month-old Murrah buffalo calves were orally dosed with the fresh aerial parts of Baccharis megapotamica var. weirii at doses of 1, 3, 4, 5 and 10g/kg body weight (bw) (~1-10mg macrocyclic trichothecenes/kg/bw). The B. megapotamica used for the experiment was harvested on a farm where a recent spontaneous outbreak of poisoning caused by such plant had occurred. Clinical signs appeared 4-20 hours and 4 buffaloes died 18-49 hours after the ingestion of the plant. Clinical signs were apathy, anorexia, and watery diarrhea, fever, colic, drooling, muscle tremors, restlessness, laborious breathing and ruminal atony, and dehydration. The most consistent gross ϐindings were restricted to the gastrointestinal (GI) tract consisted of varying degrees of edema and reddening of the mucosa of the forestomach. Histopathological ϐindings consisted of varying degrees of necrosis of the epithelial lining of the forestomach and of lymphocytes within lymphoid organs and aggregates. Fibrin thrombi were consistently found in sub-mucosal vessels of the forestomach and in the lumen of hepatic sinusoids. It is suggested that dehydration, septicemia and disseminated intravascular coagulation participate in the pathogenesis of the intoxication and play a role as a cause of death. A subsample of B. megapotamica var. weirii was frozen-dried and ground and analyzed using UHPLC (Ultra High Performance Liquid Chromatography) with high resolution Time of Flight mass spectrometry and tandem mass spectrometry, it was shown that the plant material contained at least 51 different macrocyclic trichothecenes at a total level of 1.1-1.2mg/g. About 15-20% of the total trichothecenes contents was found to be monosaccharide conjugates, with two thirds of these being glucose conjugates and one third constituted by six aldopentose conjugates (probably xylose), which has never been reported in the literature.

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“…4,27,28,30,31 Baccharis megapotamica, 15 B. coridifolia, 6,10 and B. artemisioides 22 contain a series of potent cytotoxic agents belonging to the macrocyclic trichothecene complex of antibiotics previously believed to be produced only by fungi. These cytotoxic substances were demonstrated to be the toxic principles in these plants.…”

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Baccharis megapotamica var. weirii poisoning in water buffalo (Bubalus bubalis)

et al. 2011

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Abstract. An outbreak of an acute disease in buffalo (Bubalus bubalis) caused by the ingestion of Baccharis megapotamica var. weirii occurred in the southern region of Brazil. Ten out of 50 buffalo died 24-48 hr after being introduced into a pasture containing abundant amounts of the plant. Factors influencing the ingestion of the plant and consequent toxicosis included hunger, stress caused by shipment, and unfamiliarity with the plant. Clinical signs included serous ocular discharge, incoordination, mild bloat, and muscle trembling. One buffalo was necropsied. Gross findings included dehydration, abundant liquid in the rumen, reddening of the mucosa of forestomachs, abomasum, and intestine, and edema of the wall of the rumen. The main histologic lesions were superficial to full thickness degeneration and necrosis of the stratified epithelium lining the forestomachs, necrosis of the intestinal mucosa, and widespread lymphoid necrosis. A calf (Bos taurus) was fed a single dose of 5 g/kg/body weight of B. megapotamica var. weirii harvested from the same site where the buffalo died. Twenty hours after the administration of the plant this calf died with clinical signs and lesions similar to those observed in the naturally poisoned buffalo.

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Tumor inhibitors. 125. Isolation of potent new antileukemic trichothecenes from Baccharis megapotamica

Cited by 78 publications

References 1 publication

Intoxicação espontânea por Baccharis coridifolia em bovinos

Intoxicação espontânea por Baccharis coridifolia em bovinos

Experimental poisoning by Baccharis megapotamica var. weirii in buffalo

Baccharis megapotamica var. weirii poisoning in water buffalo (Bubalus bubalis)

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