Tumor necrosis factor alfa and interleukin 1 alfa induced phosphorylation and degradation of inhibitory kappa B alpha are regulated by estradiol in endometrial cells

Arlıer, Sefa; Kayisli, Umit A.; Arıcı, Aydın

doi:10.4274/tjod.47700

Cited by 10 publications

(5 citation statements)

References 65 publications

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“…Other authors have shown that lactate increases the degradation of IκBα, promoting the translocation of NF-κB to the nucleus in human FLSs and endothelial cells [18,76]. In human FLSs, TNF-α induces the phosphorylation and degradation of IκBα [94], permitting NF-κB translocation into the nucleus to activate the transcription of target genes [43]. Additionally, Georganas et al demonstrated that the activation of NF-κB is key for the constitutive secretion of IL-6 and IL-8 as well as the secretion of these cytokines induced by IL-1β in RA-FLSs [95].…”

Section: Discussionmentioning

confidence: 94%

D-Lactate Increases Cytokine Production in Bovine Fibroblast-Like Synoviocytes via MCT1 Uptake and the MAPK, PI3K/Akt, and NFκB Pathways

Manosalva

Quiroga

Teuber

et al. 2020

Animals

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Acute ruminal acidosis (ARA) is caused by the excessive intake of highly fermentable carbohydrates, followed by the massive production of D-lactate and the appearance of neutrophilic aseptic polysynovitis. Bovines with ARA develop different lesions, such as ruminitis, polioencephalomalacia (calves), liver abscess and lameness. Lameness in cattle with ARA is closely associated with the presence of laminitis and polysynovitis. However, despite decades of research in bovine lameness as consequence of ruminal acidosis, the aetiology and pathogenesis remain unclear. Fibroblast-like synoviocytes (FLSs) are components of synovial tissue, and under pathological conditions, FLSs increase cytokine production, aggravating inflammatory responses. We hypothesized that D-lactate could induce cytokine production in bovine FLSs. Analysis by qRT-PCR and ELISA revealed that D-lactate, but not L-lactate, increased the expression of IL-6 and IL-8 in a monocarboxylate transporter-1-dependent manner. In addition, we observed that the inhibition of the p38, ERK1/2, PI3K/Akt, and NF-κB pathways reduced the production of IL-8 and IL-6. In conclusion, our results suggest that D-lactate induces an inflammatory response; this study contributes to the literature by revealing a potential key role of D-lactate in the polysynovitis of cattle with ARA.

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Section: Discussionmentioning

confidence: 94%

D-Lactate Increases Cytokine Production in Bovine Fibroblast-Like Synoviocytes via MCT1 Uptake and the MAPK, PI3K/Akt, and NFκB Pathways

Manosalva

Quiroga

Teuber

et al. 2020

Animals

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“…Transcription nuclear factor kappa B is a gene whose expression is dependent on estrogen, but it is differentially regulated in ectopic endometria compared to normal endometria. Ectopic endometria exhibits reduced immunological reactivity to kappa B inhibitors compared to normal endometria [48].…”

Section: Future Treatmentmentioning

confidence: 97%

Endometriosis and the Role of Pro-Inflammatory and Anti-Inflammatory Cytokines in Pathophysiology: A Narrative Review of the Literature

Oală,

Mitranovici,

Chiorean

et al. 2024

Diagnostics

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Endometriosis is a chronic inflammatory disease, which explains the pain that such patients report. Currently, we are faced with ineffective, non-invasive diagnostic methods and treatments that come with multiple side effects and high recurrence rates for both the disease and pain. These are the reasons why we are exploring the possibility of the involvement of pro-inflammatory and anti-inflammatory molecules in the process of the appearance of endometriosis. Cytokines play an important role in the progression of endometriosis, influencing cell proliferation and differentiation. Pro-inflammatory molecules are found in intrafollicular fluid. They have an impact on the number of mature and optimal-quality oocytes. Endometriosis affects fertility, and the involvement of endometriosis in embryo transfer during in vitro fertilization (IVF) is being investigated in several studies. Furthermore, the reciprocal influence between anti-inflammatory and pro-inflammatory cytokines and their role in the pathogenesis of endometriosis has been assessed. Today, we can affirm that pro-inflammatory and anti-inflammatory cytokines play roles in survival, growth, differentiation, invasion, angiogenesis, and immune escape, which provides a perspective for approaching future clinical implications and can be used as biomarkers or therapy.

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“…Released AA by both sPLA 2 and cPLA 2 is further activates COX-2 and oxidized to produce various proinflammatory signaling mediators such as p-cPLA 2 , p-COX-2, p-IkBα, p-p38 and MPO (Monteseirín, Bonilla et al 2001, Schieven 2005, Arlier, Kayisli et al 2018. Edematous tissues from control and treatment groups were examined for the expression levels of pro-inflammatory signaling mediators.…”

Section: Neutralization Of Spla 2 Induced Mice Paw Edema Via Downregulation Of the Expression Of Proinflammatory Signaling Mediators Exprmentioning

confidence: 99%

Syringol Isolated From Eleusine Coracana (L.) Gaertn Bran Suppresses Inflammatory Response Through the Down-Regulation of cPLA2, COX-2, IκBα, p38 and MPO Signaling in sPLA2 Induced Mice Paw Edema.

Gowda

Jayachandra

Joshi

et al. 2021

Preprint

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Eleusine coracana (L.) Gaertn (E. coracana) is one of the highest consuming food crops in Asia and Africa. E. coracana is a plant with several medicinal values including anti-ulcerative, anti-diabetic, anti-viral and anti-cancer properties. However, the anti-inflammatory property of E. coracana remains to be elucidated. Therefore, the objective of present study was to investigate the potential in isolated molecule from E. coracana via a combination of in vitro, in vivo and in silico methods. In this study we have isolated, purified and characterized an anti-inflammatory molecule from E coracana bran extract known as syringol. Purification of syringol was accomplished by combination of GC-MS and RP-HPLC techniques. Syringol significantly inhibited the enzyme activity of sPLA2 (IC50 = 3 µg) and 5-LOX (IC50 = 0.325 µg) in vitro. The inhibition is independent of substrate concentration, calcium ion concentration and was irreversible. Syringol interacts with purified sPLA2 enzymes as evidenced by fluorescence and molecular docking studies. Further, the syringol molecule dose dependently inhibited the development of sPLA2 and carrageenan induced edema. Furthermore, syringol decreases the expression of cPLA2, COX-2, IκBα, p38 and MPO in edematous tissues as demonstrated by western blots. These studies revealed that syringol isolated from E coracana bran may develop as a potent anti-inflammatory molecule.

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Tumor necrosis factor alfa and interleukin 1 alfa induced phosphorylation and degradation of inhibitory kappa B alpha are regulated by estradiol in endometrial cells

Cited by 10 publications

References 65 publications

D-Lactate Increases Cytokine Production in Bovine Fibroblast-Like Synoviocytes via MCT1 Uptake and the MAPK, PI3K/Akt, and NFκB Pathways

D-Lactate Increases Cytokine Production in Bovine Fibroblast-Like Synoviocytes via MCT1 Uptake and the MAPK, PI3K/Akt, and NFκB Pathways

Endometriosis and the Role of Pro-Inflammatory and Anti-Inflammatory Cytokines in Pathophysiology: A Narrative Review of the Literature

Syringol Isolated From Eleusine Coracana (L.) Gaertn Bran Suppresses Inflammatory Response Through the Down-Regulation of cPLA2, COX-2, IκBα, p38 and MPO Signaling in sPLA2 Induced Mice Paw Edema.

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