Tumor necrosis factor receptors (TNFRs) on T lymphocytes and soluble TNFRs in different clinical courses of sarcoidosis

Kieszko, Robert; Krawczyk, Paweł; Chocholska, Sylwia; Bojarska‐Junak, Agnieszka; Jankowska, Olga; Król, Anna; Roliński, Jacek; Milanowski, Janusz

doi:10.1016/j.rmed.2006.06.004

Cited by 19 publications

(9 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…AMs from patients with sarcoidosis have been shown to produce both receptors in higher amounts than AMs from controls. 65,66 Most interestingly, although both TNFRs are upregulated by lipopolysaccharide (LPS) stimulation and correlate with the concentration of TNF, the release of CD120b disclosed the closest link to the TNF concentration. 66 The importance of these findings was shown by Ziegenhagen et al and Hino et al demonstrating in-creased levels of CD120a and CD120b in serum and BAL fluids.…”

Section: Antigen-presenting Cellsmentioning

confidence: 99%

Sarcoidosis-Immunopathogenetic Concepts

Zissel

Prasse

Müller–Quernheim

2007

Semin Respir Crit Care Med

View full text Add to dashboard Cite

Sarcoidosis is a chronic granulomatous disorder characterized by an accumulation of activated lymphocytes, predominantly T helper cells, expressing the Th1 phenotype and macrophages at sites of disease activity. Although the cause of sarcoidosis has not been elucidated, several lines of evidence suggest that granuloma formation results from exposure to one or more antigens, eliciting a T lymphocyte response. The induction and evolution of granuloma formation results from a complex interplay between diverse cell types, cytokines, and chemokines. Genetic polymorphisms may influence the clinical expression of the granuloma formation and disease outcome. This article discusses in depth the key cellular elements and signals that generate and orchestrate the sarcoid granulomatous response. The precise factors inciting the sarcoid granulomatous response have not yet been identified, but chronic exposure to microbial agents, their products, or inorganic substances may be important in the pathogenesis.

show abstract

Section: Antigen-presenting Cellsmentioning

confidence: 99%

Sarcoidosis-Immunopathogenetic Concepts

Zissel

Prasse

Müller–Quernheim

2007

Semin Respir Crit Care Med

View full text Add to dashboard Cite

show abstract

“…TNFR1 (CD120a) is constitutively, but in low levels, expressed on nearly all nucleated cell types. TNFR2 (CD120b) is inducible and expressed by cells of myeloid lineage, peripheral T cells and alveolar lymphocytes and macrophages [18-23]. TNFR1 activates the caspase family, which induce cell death.…”

Section: Introductionmentioning

confidence: 99%

“…Approximately 25% of the peripheral blood lymphocytes in healthy subjects is CD4 + TNFR2 + . Interestingly, in sarcoidosis the percentage of CD4 + TNFR2 + cells is increased, especially in patients in remission or with stable disease (respectively 35% and 33%), which may indicate a role in down regulation of a cell-mediated immune response [23]. FoxP3 + T reg cells expressed the highest levels of TNFR2 among subsets of human peripheral blood CD4 + T cells and were able to shed large amounts of sTNFR2, suggesting an important role for T reg cells in suppressing TNF [25].…”

Section: Introductionmentioning

confidence: 99%

Differential expression of TNFR1 (CD120a) and TNFR2 (CD120b) on subpopulations of human monocytes

et al. 2012

View full text Add to dashboard Cite

BackgroundThree subpopulations of monocytes can be distinguished in human blood: classical (CD14++CD16−), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++). CD16 expressing monocytes are expanded in patients with sarcoidosis and in various other inflammatory diseases. In sarcoidosis, it is unclear whether either intermediate, nonclassical or both CD16 expressing monocytes are responsible for this increase. Data relating to the monocyte subpopulations is receiving increasing attention, but the expression of TNF receptors on these subpopulations has not been studied thus far. The aim of this study was to determine frequencies of monocyte subpopulations and their expression of TNFR1 and TNFR2 in both sarcoidosis patients and healthy controls.MethodsPeripheral blood cells of sarcoidosis patients and healthy controls were stained for the markers HLA-DR, CD14, CD16, CD120a and CD120b. Cells were measured on a FACSCalibur and analyzed with FlowJo. We used Student’s t-test and a parametric One-way ANOVA for statistical analysis.ResultsSarcoidosis patients had a significant higher frequency of intermediate monocytes than healthy controls. Significant differences in TNF receptor expression were found between the monocyte subpopulations, both in sarcoidosis patients as well as in healthy controls: intermediates expressed more TNFR1 than classicals and nonclassicals and nonclassicals expressed more TNFR2 than intermediates, whereas intermediates showed higher expression than classicals.ConclusionsIn both sarcoidosis patients and healthy controls intermediate monocytes show the highest expression level of TNFR1 among monocyte subpopulations and nonclassical monocytes show the highest expression level of TNFR2. These findings, as wells as the higher frequency of intermediate monocytes in sarcoidosis patients, provide evidence for the existence of two functionally-distinct CD16 expressing monocyte subpopulations.

show abstract

“…Possible serum markers including tumour necrosis factor receptor II have been proposed. 11 The Kveim test involves an intradermal injection of homogenised human sarcoid tissue extract. Although it has 50% sensitivity and close to 100% specificity, because of the impracticality of using human tissue the test is no longer used.…”

Section: Long Answermentioning

confidence: 99%