Tumor Neoangiogenesis by CD31 and CD105 Expression Evaluation in Breast Carcinoma Tissue Microarrays

Charpin, Colette; Dalès, Jean-Philippe; García, Stéphane; Carpentier, Samuel; Djemli, Amina; Andrac, L; Lavaut, Marie‐Noëlle; Allasia, Claude; Bonnier, Pascal

doi:10.1158/1078-0432.ccr-04-0021

Cited by 43 publications

(39 citation statements)

References 23 publications

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“…The appropriate threshold of prognostic significance for a given marker was determined as previously recommended 33 and described. 22,[24][25][26][27][28][29][30][31] Logistic regression (with ROC curves) was then used to identify the combination of markers with the best sensitivity and specificity indicative of a proteomic signature of poor prognosis.…”

Section: Discussionmentioning

confidence: 99%

“…22,31 Briefly, TMA analysis with a SAMBA 2050 automated device (SAMBA / TRIBVN, Châtillon 92320, France) [24][25][26][27] was performed according to the following protocol.…”

Section: Image Analysismentioning

confidence: 99%

“…From the images acquired with 203 magnification, a new set of images was next computed, one for each core. For color analysis of the core images, the SAMBA ''immuno'' software was applied as previously reported [24][25][26][27][28][29][30][31] in usual full tissue sections.…”

Section: Image Analysismentioning

confidence: 99%

“…In positive (>0) cases, optimal thresholds greater than zero, specific for each marker, were determined according to the p value curves from univariate analysis (log rank), as reported by Altman et al 33 and previously used, 22,[24][25][26][27][28][29][30][31] as shown in Figure 2 and Table III. Table III shows that 27 of the 42 prognostic markers were significant in the univariate analysis and Figure 3 illustrates an unsupervised hierarchical clustering of prognostic significant markers in the log rank test.…”

Section: Quantitative Score and Survivalmentioning

confidence: 99%

“…In particular, quantification of immunoprecipitates with automated computer-assisted devices relying on densitometry allows more objective analysis of results. [24][25][26][27][28][29][30][31] Also, quantitative data are more appropriate for statistical analysis and permit more valid studies, although some variation in interpretation of results can still occur. 32 Our objective in the present study was to determine immunohistochemical criteria for phenotyping of tumors of poor prognosis, and particularly for metastatic risk that would be economically applicable for individual patients with breast carcinoma, using the latest methodology for standardization and quality control.…”

mentioning

confidence: 99%

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A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays

Charpin

Secq

Giusiano

et al. 2009

Intl Journal of Cancer

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Quantitative immunocytochemical assays of 1,200 breast carcinomas were assessed after construction of tissue microarrays. A total of 42 markers were evaluated for prognostic significance by univariate log rank test (mean follow‐up, 79 months), using quantitative scoring by an image analysis device and specific software. Complete data were obtained for 924 patients, for whom 27 of the 42 markers proved to be significant prognostic indicators. Analysis of these 27 markers by logistic regression showed that 18 (cMet, CD44v6, FAK, moesin, caveolin, c‐Kit, CK14, CD10, P21, P27, pMAPK, pSTAT3, STAT1, SHARP2, FYN, ER, PgR and c‐erb B2), and 15 when ER, PgR and c‐erb B2 were excluded, were 80.52% and 78.9% predictive of disease outcome, respectively. The immunocytochemical assays on 4 micron thick sections of fixed tissue are easy to handle in current practice and are cost‐effective. Quantitative densitometric measurement of immunoprecipitates by computer‐assisted devices from digitized microscopic images allows standardized high‐throughput “in situ” molecular profiling within tumors. It is concluded that this 15 marker immunohistochemical signature is suitable for current practice, since performed on paraffin sections of fixed tumor samples, and can be used to select patients needing more aggressive therapy, since this signature is about 80% predictive of poor clinical outcome. Also, the markers included in the signature may be indicative of tumor responsiveness to current chemotherapy or suggest new targets for specific therapies. © 2008 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

“…22,31 Briefly, TMA analysis with a SAMBA 2050 automated device (SAMBA / TRIBVN, Châtillon 92320, France) [24][25][26][27] was performed according to the following protocol.…”

Section: Image Analysismentioning

confidence: 99%

Section: Image Analysismentioning

confidence: 99%

Section: Quantitative Score and Survivalmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays

Charpin

Secq

Giusiano

et al. 2009

Intl Journal of Cancer

Self Cite

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Overcoming Hypoxia by Multistage Nanoparticle Delivery System to Inhibit Mitochondrial Respiration for Photodynamic Therapy

Xia

Luo

et al. 2019

Adv Funct Materials

149

112

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Hypoxia, as characterized by the low local oxygen, confers on cancer cells resistance to oxygen-consuming photodynamic therapy (PDT). The limited success reached by current approaches harnessing reoxygenation to enhance PDT outcome promotes the reconsideration of the design of the therapeutic approach. In this study, a multistage delivery system capable of reversing hypoxia is demonstrated. Unlike previous strategies that only expect to affect the peripheral tumor tissue, the size-shrinkable system allows those deeply located hypoxia regions to be treated. Specifically, therapeutics, including atovaquone and indocyanine green derivatives that are respectively responsible for oxidative phosphorylation blockage and PDT, are encapsulated in a gelatin nanoparticle, whose structure would rupture to promote deep penetration when facing matrix metallopeptidase 2 enzyme overexpression in tumor tissue. The antihypoxic performance of the platform has been evaluated using a variety of analyses including flow-cytometry assay, immunofluorescence, and micro-positron-emission tomography imaging. Tumor regression in animal models confirms the feasibility and effectiveness of conquering the PDT-resistance through abrogating the oxygen consumption. It is hopeful that such a strategy could shed light on the development of next-generation PDT-adjuvant treatment.

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Anti‐tumor activity of an anti‐endoglin monoclonal antibody is enhanced in immunocompetent mice

Tsujie

Toi

et al. 2008

Intl Journal of Cancer

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In the present study, we investigated the mechanisms by which anti-endoglin (EDG; CD105) monoclonal antibodies (mAbs) suppress angiogenesis and tumor growth. Antihuman EDG mAb SN6j specifically bound to murine endothelial cells and was internalized into the cells in vitro. SN6j effectively suppressed angiogenesis in mice in the Matrigel plug assay. We found that SN6j is more effective for tumor suppression in immunocompetent mice than in SCID mice. We hypothesized that T cell immunity is important for effective antitumor efficacy of SN6j in vivo. To test this hypothesis, we investigated effects of CpG oligodeoxynucleotides (ODN) and depletion of CD4 1 T cells and/or CD8 1 T cells on antitumor efficacy of SN6j in mice. Systemic (i.v.) administration of a relatively small dose (0.6 lg/g body weight/dose) of SN6j suppressed growth of established s.c. tumors of colon-26 in BALB/c mice and improved survival of the tumor-bearing mice. Addition of CpG ODN to SN6j synergistically enhanced antitumor efficacy of SN6j. In contrast, such enhancing effects of CpG ODN were not detected in SCID mice. Antitumor efficacy of SN6j in BALB/c mice was abrogated when CD41 T cells and/or CD8 1 T cells were depleted; effect of CD8 1 T cell depletion was stronger. Interestingly, CD4-depletion decreased tumor growth while CD8-depletion enhanced tumor growth in the absence of SN6j. SN6j induced apoptosis in human umbilical vein endothelial cells in a dose-dependent manner which indicates an additional mechanism of antiangiogenesis by SN6j. ' 2008 Wiley-Liss, Inc.Key words: endoglin; antiangiogenesis; monoclonal antibody therapy; T cell immunity; synergy Angiogenesis is essential for tumor growth, progression and metastasis and antiangiogenic therapy has become a new promising strategy in the treatment of cancer patients.

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Tumor Neoangiogenesis by CD31 and CD105 Expression Evaluation in Breast Carcinoma Tissue Microarrays

Cited by 43 publications

References 23 publications

A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays

A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays

Overcoming Hypoxia by Multistage Nanoparticle Delivery System to Inhibit Mitochondrial Respiration for Photodynamic Therapy

Anti‐tumor activity of an anti‐endoglin monoclonal antibody is enhanced in immunocompetent mice

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