Tumor promoters induce a specific morphological signature in the nuclear matrix-intermediate filament scaffold of Madin-Darby canine kidney (MDCK) cell colonies.

Fey, Edward G.; Penman, Sheldon

doi:10.1073/pnas.81.14.4409

Cited by 60 publications

(30 citation statements)

References 26 publications

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“…While in SMMC-7721 cells induced with HMBA, the nuclear matrix filaments and intermediate filaments interweaved into a regular and well-distributed meshwork in which the quantity of single filaments increased and differed in slender and thick form, moreover, the nuclear matrix filaments and intermediate filaments firmly fastened to the thin and compact fiber-like nuclear lamina. This morphology of NM-IF system was significantly different from those of rapidly proliferating SMMC-7721 cells but similar to those of some previously reported epithelial or normal cells [15,18] , and was consistent with our previous studies in which we treated the human osteosarcoma cell line MG-63 and human gastric adenocarcinoma cell line MGC80-3 with retinotic acid (RA) respectively [12,19] . It showed that the morphology and function of NM-IF system in SMMC-7721 cells during its differentiation had undergone a reverse alteration.…”

Section: Smmc-7721supporting

confidence: 78%

Alteration of nuclear matrix-intermediate filament system and differential expression of nuclear matrix proteins during human hepatocarcinoma cell differentiation

Tang¹,

Niu²,

Xu³

et al. 2007

WJG

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AIM:To investigate the association between the configurational and compositional changes of nuclear matrix and the differentiation of carcinoma cells. METHODS:Cells cultured with or without 5 × 10 -3 mmol/L of hexamethylene bisacetamide (HMBA) on Nickel grids were treated by selective extraction and prepared for whole mount observation under electron m i c ro s c o p y. T h e s a m p l e s w e re e x a m i n e d u n d e r transmission electron microscope. Nuclear matrix proteins were selectively extracted and subjected to subcellular proteomics study. The protein expression patterns were analyzed by PDQuest software. Spots of differentially expressed nuclear matrix proteins were excised and subjected to in situ digestion with trypsin.The peptides were analyzed by matrix-assisted laserdesorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Data were submitted for database searching using Mascot tool (www.matrixscience.com). RESULTS:The nuclear matrix (NM) and intermediate filament (IF) in SMMC-7721 hepatocarcinoma cells were found relatively sparse and arranged irregularly. The nuclear lamina was non-uniform, and two kinds of filaments were not tightly connected. After induction for differentiation by HMBA, the NM-IF filaments were concentrated and distributed uniformly. The heterogeneous population of filaments, including highly branched utrathin filaments could also be seen in the regular meshwork. The connection between the two kinds of filaments and the relatively thin, condensed and sharply demarcated lamina composed of intermediatesized filaments was relatively fastened. Meanwhile, 21 NM proteins changed remarkably during SMMC-7721 cell differentiation. Four proteins, i.e. mutant Pyst1, hypothetical protein, nucleophosmin1, and LBP were downregulated, whereas four other proteins, eIF6, p44 subunit, β-tubulin, and SIN3B were upregulated with the last one, SR2/ASF found only in the differentiated SMMC-7721 cells. CONCLUSION:The induced differentiation of SMMC-7721 cells by HMBA is accompanied by the configurational changes of nuclear matrix-intermediate filament (NM-IF) system and the compositional changes of nuclear matrix protein expression. These changes may be important morphological or functional indications of the cancer cell reversion.

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Section: Smmc-7721supporting

confidence: 78%

Alteration of nuclear matrix-intermediate filament system and differential expression of nuclear matrix proteins during human hepatocarcinoma cell differentiation

Tang¹,

Niu²,

Xu³

et al. 2007

WJG

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“…Furthermore, nuclear matrix proteins have been implicated in malignant transformation (18,19), such as alterations in DNA ploidy, DNA content, nuclear shape, and proliferative states (20). The nuclear matrix protein, termed NRP/B (ENC-1), is expressed abundantly in the nervous system (21,22), and its expression has been observed in various brain cell lines, human brain tumors (23,24), and hairy cell leukemia (25).…”

Section: Introductionmentioning

confidence: 99%

The Nuclear Matrix Protein, NRP/B, Enhances Nrf2-Mediated Oxidative Stress Responses in Breast Cancer Cells

et al. 2007

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The transcription factor NF-E2-related factor 2 (Nrf2) translocates into the nucleus and activates phase II genes encoding detoxification enzymes and antioxidant proteins, resulting in the protection of cells from oxidative insults. However, the involvement of Nrf2-mediated oxidative stress responses in breast cancer cells is largely unknown. Notably, during our study of the Nrf2 pathway in breast cancer cells, we observed that the nuclear matrix protein NRP/B was expressed and colocalized with Nrf2 in these cells, suggesting that NRP/B is involved in Nrf2-mediated oxidative stress responses. The expression level of NRP/B was variable in different breast cancer cells and breast cancer tissues, and was found to be localized in the nucleus. NRP/B expression was increased after exposure to the oxidative stress agent, hydrogen peroxide (H 2 O 2 ), particularly in the highly aggressive MDA-MB-231 breast cancer cells. Association of NRP/B with Nrf2 in vitro and in vivo was observed in MDA-MB-231 breast cancer cells, and this association was up-regulated upon exposure to H 2 O 2 , but not to sodium nitroprusside, SIN-1, and DETA-NO. NRP/B also enhanced Nrf2-mediated NAD(P)H:quinine oxidoreductase 1 promoter activity. Thus, this study reveals that NRP/B enhances oxidative stress responses in breast cancer cells via the Nrf2 pathway, identifying a novel role of nuclear matrix protein(s) in oxidative stress responses. [Cancer Res 2007;67(18):8596-604]

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“…These proteins have been implicated in malignant transformation (Fey and Penman, 1984;Getzenberg et al, 1996), such as alterations in DNA ploidy, DNA content, nuclear shape and proliferative states (Berezney et al, 1995). The nuclear matrix protein termed nuclear restricted protein in brain (NRP/B) (Kim et al, 1998), also known as ENC-1 (Hernandez et al, 1997), was characterized as a Kelch-related protein.…”

Section: Introductionmentioning

confidence: 99%

NRP/B mutations impair Nrf2-dependent NQO1 induction in human primary brain tumors

et al. 2008

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Brain tumors are associated with genetic alterations of oncogenes and tumor suppressor genes. Accumulation of reactive oxygen species (ROS) in cells leads to oxidative stress-induced damage, resulting in tumorigenesis. Here, we showed that the nuclear matrix protein nuclear restricted protein in brain (NRP/B) was colocalized and interacted with NF-E2-related factor 2 (Nrf2). During oxidative stress response, NRP/B expression and its interaction with Nrf2 were upregulated in SH-SY5Y cells. Association of NRP/B with Nrf2 was crucial for NAD(P)H:quinone oxidoreductase 1 (NQO1) expression. NRP/B was localized predominantly in the nucleus of normal brain cells, whereas in primary brain tumors NRP/B was almost exclusively contained in the cytoplasm. In addition, unlike wild-type NRP/B, the expression of NRP/B mutants isolated from primary brain tumors was found in the cytoplasm, and these mutants failed to induce Nrf2-dependent NQO1 transcription. Thus, NRP/B mutations and their altered localization resulted in changes in NRP/B function and deregulation of Nrf2-dependent NQO1 activation in brain tumors. This study provides insights into the mechanism by which the NRP/B modulates Nrf2-dependent NQO1 induction in cellular protection against ROS in brain tumors.

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Tumor promoters induce a specific morphological signature in the nuclear matrix-intermediate filament scaffold of Madin-Darby canine kidney (MDCK) cell colonies.

Abstract: Tumor promoters such as phorbol 12-tetradecanoate 13-acetate (TPA), mezerein, teleocidin, aplysiatoxin, and benzoyl peroxide, although structurally unrelated

Cited by 60 publications

References 26 publications

Alteration of nuclear matrix-intermediate filament system and differential expression of nuclear matrix proteins during human hepatocarcinoma cell differentiation

Alteration of nuclear matrix-intermediate filament system and differential expression of nuclear matrix proteins during human hepatocarcinoma cell differentiation

The Nuclear Matrix Protein, NRP/B, Enhances Nrf2-Mediated Oxidative Stress Responses in Breast Cancer Cells

NRP/B mutations impair Nrf2-dependent NQO1 induction in human primary brain tumors

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