Tumor-specific membrane antigens in established cell lines from gliomas

Wahlström, Torsten; Linder, Ewert; Saksela, Eero; Westermark, Bengt

doi:10.1002/1097-0142(197408)34:2<274::aid-cncr2820340209>3.0.co;2-4

Cited by 50 publications

(6 citation statements)

References 17 publications

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“…U251 grows in monolayer, is anchorage independent in soft agar and grows as a xenograft in athymic mice. 3,47,48 By comparison, U343 is an obligate, anchorage-dependent tumor cell line which is non-invasive. 4 GFAP-negative U251 astrocytoma cells (asU251) were obtained following the transfection of parental U251 cells with an expression vector carrying a murine complementary DNA for GFAP in the antisense orientation as described previously.…”

Section: Astrocytoma Cell Lines and Culture Conditionsmentioning

confidence: 99%

Co‐expression of nestin and vimentin intermediate filaments in invasive human astrocytoma cells

Rutka

Ivanchuk

Mondal

et al. 1999

Intl J of Devlp Neuroscience

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Intermediate filaments (IFs) are highly diverse intracytoplasmic proteins within the cytoskeleton which exhibit cell type specificity of expression. A growing body of evidence suggests that IFs may be involved as collaborators in complex cellular processes controlling astrocytoma cell morphology, adhesion and proliferation. As the co-expression of different IF subtypes has been linked to enhanced motility and invasion in a number of different cancer subtypes, we undertook the present study to examine the expression of vimentin and nestin in a panel of human astrocytoma cell lines whose tumorigenicity, invasiveness and cytoskeletal protein profiles are well known. Astrocytoma cells were examined for IF protein expression by immunofluorescence confocal and immunoelectron microscopy. The motility of all cell lines was determined by computerized time-lapse videomicroscopy. Invasive potential of astrocytoma cells was determined using Matrigel as a barrier to astrocytoma cell invasion in vitro. Vimentin was expressed by all astrocytoma cell lines. On the other hand, nestin was variably expressed among the different cell lines. The most motile and invasive astrocytoma cell line in our study was antisense GFAP-transfected U251 (asU251) astrocytoma cells which showed marked up-regulation of nestin expression compared to the U251 parental cell line and controls. The U87 astrocytoma cell line also demonstrated high nestin expression levels and was associated with an increased basal motility rate and a high degree of invasiveness through Matrigel. U343 astrocytoma cells did not express nestin, but had high levels of GFAP. It had the lowest motility rate and invasiveness of all the astrocytoma cell lines examined. Taken together, these data suggest that for the astrocytoma cell lines examined in this study, nestin and vimentin co-expression may serve as a marker for an astrocytoma cell type with enhanced motility and invasive potential. Further studies are required to determine the mechanism by which dual-IF protein expression alters other cytoskeletal or cell surface receptor protein components important in the process of astrocytoma invasion.

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Section: Astrocytoma Cell Lines and Culture Conditionsmentioning

confidence: 99%

Co‐expression of nestin and vimentin intermediate filaments in invasive human astrocytoma cells

Rutka

Ivanchuk

Mondal

et al. 1999

Intl J of Devlp Neuroscience

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“…The possibility that gliomas might be diagnosed by the detection of human gliomaassociated antigen was raised by Mahaley & Day (1965). Since then a number of antigens, apparently specific for human astrocytomas have been found (Wahlstrom et al 1974, Coakham & Lakshmi 1975, Miyake & Kitamura r977), although specific reactivity has been rarely demonstrated (see review Wikstrand & Bigner 1980). Evidence of a human glioblastoma specific antigen has also been provided by Kehayov (r976), and more recently monoclonal antibodies which are apparently specific to glioma have been produced by lymphocytes derived from biopsies of glioma and then fused to form hybridomas with cultured human myeloma cells (Sikora et al 1982).…”

Section: G L I O M a Associated A N T I G E N I N H U M A N G L I O M Amentioning

confidence: 99%

Nervous system antigens

1983

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Some of the better characterized proteins (markers) of the nervous system are described. The availability of specific antibodies to these markers has allowed the localization and assay of the proteins in tissue and biological fluids. There is some evidence that autosensitization may occur. Clinical application of these markers includes the evaluation and prognostic significance after stroke and head injury. The diagnostic histopathological use of the markers in the investigation of various tumours is summarized.

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“…The results of the experiments presented here and summarised in table 6 indicate that dissociated cells from astrocytomas of various degrees of malignancy do not express HLA-D/DR determinants. The evidence for this is two-fold; the tumour cells do not stimulate allogeneic lymphocytes and anti-HLA-DR antibodies do not bind to the tumour cells.…”

Section: Discussionmentioning

confidence: 71%

Histocompatibility antigens on astrocytoma cells.

Hirschberg¹,

Endresen²,

Wikeby³

1982

Journal of Neurology, Neurosurgery & Psychiatry

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SUMMARY Biopsied tumour cells from astrocytoma-bearing patients were grown in primary culture for 3-5 days. Both low and high grade tumours were represented in the study. The cultured cells could be shown to express the HLA-A and -B antigens using a multispecific allo-antiserum and a rabbit anti-f-2 microglobulin antibody. The tumour cells were negative for the HLA-DR determinants when tested with either rabbit anti-Ia-like antisera or specific anti-HLA-DR allo-antisera. They also failed to stimulate allogeneic lymphocytes in primary mixed lymphocyte-tumour cell cultures but stimulated lymphocytes primed to tumour cells in vitro. The tumour cells were also capable of stimulating autologous lymphocytes from the tumour-bearing patient in most of the combinations tested. Materials and methodsPatients Twenty-two patients with various grades of astrocytomas (I-IV) were used in this study. The grading system described in the Kernohan classification has been used throughout this paper.12 13 Tumour biopsies were obtained at the time of operative resection and peripheral blood samples were obtained during the first 3 days after operation. All of the patients were receiving steroid treatment (Dekadron) at various doses pre-operatively, but none after operation.

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Tumor-specific membrane antigens in established cell lines from gliomas

Cited by 50 publications

References 17 publications

Co‐expression of nestin and vimentin intermediate filaments in invasive human astrocytoma cells

Co‐expression of nestin and vimentin intermediate filaments in invasive human astrocytoma cells

Nervous system antigens

Histocompatibility antigens on astrocytoma cells.

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