Tumor-specific transplantation and surface antigen in cells transformed by the adenovirus 12 DNA fragments

Shiroki, Kazuko; Shimojo, Hiroto; Maeta, Yasunobu; Hamada, Chuya

doi:10.1016/0042-6822(79)90053-9

Cited by 19 publications

(4 citation statements)

References 8 publications

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“…Persson et al (39) found the polypeptide to be present in both nuclear and plasma membrane fractions of infected cells. Shiroki et al (45) and Raska et al (40,41) have mapped an adenovirus tumor-specific transplantation antigen to early region 1, and Gallimore and Williams (16) have employed region El mutants to localize the antigen to the ElB-21K coding region. Thus, the antigen likely plays a key role at the interface between the infected or tumor cell and the host immune surveillance system.…”

Section: Discussionmentioning

confidence: 99%

Deletion of the gene encoding the adenovirus 5 early region 1b 21,000-molecular-weight polypeptide leads to degradation of viral and host cell DNA

Pilder¹,

Logan²,

Shenk³

1984

J Virol

181

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The adenovirus 5 mutant H5dl337 lacks 146 base pairs within early region 1B. The deletion removes a portion of the region encoding the ElB 21,000-molecular-weight (21K) polypeptide, but does not disturb the ElB-55K/17K coding region. The virus is slightly defective for growth in cultured HeLa cells, in which its final yield is reduced ca. 10-fold compared with wild-type virus. The mutant displays a striking phenotype in HeLa cells. The onset of cytopathic effect is dramatically accelerated, and both host cell and viral DNAs are extensively degraded late after infection. This defect has been described previously for a variety of adenovirus mutants and has been termed a cytocidal (cyt) phenotype. H5dl337 serves to map this defect to the loss of E1B-21K polypeptide function. In addition to its defect in the productive growth cycle, H5dl337 is unable to transform rat cells at normal efficiency.

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Section: Discussionmentioning

confidence: 99%

Deletion of the gene encoding the adenovirus 5 early region 1b 21,000-molecular-weight polypeptide leads to degradation of viral and host cell DNA

Pilder¹,

Logan²,

Shenk³

1984

J Virol

181

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“…The EIB 19-kDa product is associated with transformed cell membranes and, along with a different 19-kDa protein encoded by Ad early region 3 (located between 76.6 and 86.0 m.u. ), may be involved in expression of virus-specific cell-surface transplantation antigen (23,(25)(26)(27)(28)(29).…”

Section: Methodsmentioning

confidence: 99%

Expression of the adenovirus E1A oncogene during cell transformation is sufficient to induce susceptibility to lysis by host inflammatory cells.

Cook¹,

Walker²,

Lewis³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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Mammalian cells transformed by nononcogenic human adenoviruses exhibit high susceptibility to destruction by host mononuclear inflammatory cells. We have analyzed the viral gene regulation of the susceptibility of transformed cells to lysis by natural killer cells and activated macrophages. Comparisons of target cell lines transformed by overlapping segments of the adenovirus El-transforming gene region revealed that isolated expression of a single oncogene, EIA, was sufficient to cause increased cytolytic susceptibility in the absence of detectable transformed cell-surface expression of viral transplantation antigens and irrespective of histocompatibility antigen identity between killer cells and target cells. These results suggest that oncogene functions that are not linked to the expression of previously recognized cell-surface target structures may actively induce neoplastic cell elimination by components of the host immune surveillance system.

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“…In the earlier studies of effects of immunization with cells transformed with isolated fragments of Adl2 DNA, the Acc I-H fragment (0-4.7 map units) transformants failed to induce tumor immunity seen with cells transformed with the Hind III-G fragment (0-6.8 map units). The TSTA function was tentatively assigned to the left end of the E 1 B region (10). Analysis of a library of syngeneic cells transformed in vitro with the cloned fragments of the transforming region revealed that cells transformed with Eco RI-C fragment (0-16.5 map units), Sal I-C fragment (0-10.3 map units), and Hind III-G fragment (0-6.8 map units) elicit cytolytic responses (11).…”

Section: Discussionmentioning

confidence: 99%

Adenovirus tumor-specific transplantation antigen is a function of the E1A early region.

Sawada¹,

Urbanelli²,

Rašková³

et al. 1986

The Journal of Experimental Medicine

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Viable recombinant adenoviruses that carry a portion of the type 12 E1A and E1B transcription units in a type 5 background were used to identify genes controlling expression of the adenovirus tumor-specific transplantation antigen (TSTA). The TSTA immunity is not crossreacting between the group A and group C adenovirus serotypes. Viruses carrying the E1A region (sub370-12E1A), or both E1A and E1B (sub370-12E1AB) regions of Ad12, induce a strong transplantation immunity against tumors induced by syngeneic cells transformed with adenovirus 12, but fail to induce any protection against syngeneic cells transformed with adenovirus 2. Immunization with the virus carrying only the E1B region (sub370-12E1B) of adenovirus 12 induces no immunity to adenovirus 12 transformed cell line, but confers a strong protection against cells transformed with adenovirus 2. These results provide strong evidence that the adenovirus tumor-specific transplantation antigen is a function of the E1A early region.

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Tumor-specific transplantation and surface antigen in cells transformed by the adenovirus 12 DNA fragments

Cited by 19 publications

References 8 publications

Deletion of the gene encoding the adenovirus 5 early region 1b 21,000-molecular-weight polypeptide leads to degradation of viral and host cell DNA

Deletion of the gene encoding the adenovirus 5 early region 1b 21,000-molecular-weight polypeptide leads to degradation of viral and host cell DNA

Expression of the adenovirus E1A oncogene during cell transformation is sufficient to induce susceptibility to lysis by host inflammatory cells.

Adenovirus tumor-specific transplantation antigen is a function of the E1A early region.

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