Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling

Ye, Han; Wu, Zhenzhen; Wu, Tingfeng; Huang, Yulun; Cheng, Zhen; Li, X; Sun, Ting; Xie, Xing; Zhou, Youxin; Du, Ziwei

doi:10.1038/cddis.2015.407

Cited by 204 publications

(164 citation statements)

References 36 publications

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“…And downregulation of MALAT1 affects proliferation and the expression of stemness markers in glioma stem cell line SHG139S [30]. In addition, the potential employment of lncRNAs as novel biomarkers is supported by widespread observations [31]. Previous study has indicated the promotive roles of MALAT1 in gastric tumor [9], here we further confirmed this idea and showed that overexpression of MALAT1 could attenuate or even reverse the inhibitory effects of UPF1 on gastric tumor.…”

Section: Discussionsupporting

confidence: 76%

The Human RNA Surveillance Factor UPF1 Modulates Gastric Cancer Progression by Targeting Long Non-Coding RNA MALAT1

Li¹,

Geng²,

Ren³

et al. 2017

Cell Physiol Biochem

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Background/Aims: The long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is overexpressed in numerous cancers. However, whether MALAT1 is regulated and the related mechanisms in gastric cancer remain unclear. Methods: Immunohistochemistry and qRT-PCR analyses were used to detect the expression levels of UPF1 and MALAT1 in gastric cancer and adjacent normal tissues. MTT, cell cycle, apoptosis and transwell assays were performed to examine the effects of UPF1 on cell cycle progression, cell proliferation, apoptosis, migration and invasion. Additionally, sodium bisulfate sequencing was used to test the promoter hypermethylation on UPF1 in gastric tumor tissues. Finally, RNA immunoprecipitation and luciferase reporter analyses demonstrated that UPF1 directly bound with MALAT1. Results: The expression of UPF1 was significantly downregulated in gastric cancer and negatively correlated with MALAT1 expression. Patients with lower expression of UPF1 had poorer prognosis than those with higher expression. Overexpression of UPF1 inhibited cell proliferation, cell cycle progression, cell migration and invasion, and promoted cell apoptosis in gastric cancer cells. Moreover, the UPF1-mediated inhibition of gastric cancer progression was reversed by overexpression of MALAT1. A profound downregulation of UPF1 in gastric tumor tissues was due to promoter hypermethylation. Overexpression of UPF1 increased nonsense-mediated mRNA decay (NMD) efficiency and thus led to downregulation of MALAT1. Conclusion: Our results demonstrate that UPF1 is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer.

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Section: Discussionsupporting

confidence: 76%

The Human RNA Surveillance Factor UPF1 Modulates Gastric Cancer Progression by Targeting Long Non-Coding RNA MALAT1

Li¹,

Geng²,

Ren³

et al. 2017

Cell Physiol Biochem

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“…Compared with the CC genotype, the CT and TT genotypes both increased the risk of T-ALL. MMP-2 is one of the factors in the MMP family that is closely associated with tumor progression [31, 32]. In addition to the degradation of type IV collagen in the basement membrane, MMP-2 interacts with other biologically active molecules, such as growth factors binding proteins and growth factor receptors [33].…”

Section: Discussionmentioning

confidence: 99%

The Relationship Between MMP-2 -1306C>T and MMP-9 -1562C>T Polymorphisms and the Risk and Prognosis of T-Cell Acute Lymphoblastic Leukemia in a Chinese Population: A Case-Control Study

Lin

Zeng

Xiao

et al. 2017

Cell Physiol Biochem

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Background: T-cell acute lymphoblastic leukemia (T-ALL) is a malignant hematological disease and is often accompanied by a variety of genetic abnormalities. Hence, our study aims to investigate the relationship between MMP-2 -1306C>T and MMP-9 -1562C>T polymorphisms and the risk and prognosis of T-ALL. Methods: From April 2009 to February 2011, a total of 376 T-ALL patients were chosen as the case group. Meanwhile, 352 healthy people who passed routine health examinations were selected as the control group. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to detect the frequency of MMP-2 -1306C>T (rs243865) and MMP-9 -1562C>T (rs3918242) polymorphisms in the study subjects. The serum levels of MMP-2 and MMP-9 were detected using enzyme-linked immunosorbent assay (ELISA). A Kaplan-Meier analysis was employed to analyze the event-free survival (EFS) rates of the T-All patients with different MMP-2 and MMP-9 genotypes. A multivariate COX model was applied to analyze the relationship between MMP-2 and MMP-9 polymorphisms and the prognosis of T-ALL patients. A C-statistic and net reclassification index (NRI) was carried out to evaluate the predictive value of MMP-2 and MMP-9 gene polymorphisms using the Cox model. Results: Compared to the control group, the genotypic frequency of MMP-2 -1306C>T (CT + TT) and MMP-9 -1562C>T (CT + TT) in the case group was significantly higher. The serum level of MMP-9 was markedly elevated in T-ALL patients with the CT + TT genotype compared to patients with the CC genotype. The results of the Kaplan-Meier analysis showed that the median EFS was lower in T-ALL patients with the CT + TT genotype of MMP-9 -1562C>T compared to patients with the CC genotype. The results of a multivariate analysis using the Cox proportional hazard model indicated that the MMP-9 -1562C>T polymorphism was associated with the prognosis of T-ALL patients. Conclusion: These results indicated that MMP-2 -1306C/T and MMP-9 -1562C/T polymorphisms might be associated with an increased risk of T-ALL. The MMP-9 -1562C>T polymorphism may also be related to the prognosis of T-ALL patients.

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“…LncRNAs are closely associated with tumor development (9,10) and particularly, have been implicated in glioma progression (11). For example, the lncRNA, MALAT1, has been demonstrated to have crucial roles in the cell proliferation, metastasis and apoptosis processes in glioma (12)(13)(14). MALAT1 was proposed as a tumor suppressor in glioma (14).…”

Section: Introductionmentioning

confidence: 99%

“…For example, the lncRNA, MALAT1, has been demonstrated to have crucial roles in the cell proliferation, metastasis and apoptosis processes in glioma (12)(13)(14). MALAT1 was proposed as a tumor suppressor in glioma (14). Knockdown of MALAT1 increases the blood-tumor barrier permeability (15), and affects proliferation and the expression of stemness markers in glioma (12).…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA eosinophil granule ontogeny transcript inhibits cell proliferation and migration and promotes cell apoptosis in human glioma

Liang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Glioma is the most common primary brain tumor and represents one of the most aggressive and lethal types of human cancer. Recent advances have implicated long noncoding RNAs (lncRNAs) as crucial mediators of cancer development and progression. The present study aimed to investigate the role of a newly-discovered lncRNA, termed eosinophil granule ontogeny transcript (EGOT), in the aggressive abilities of cells in human glioma. It was initially found that the relative transcription level of EGOT in glioma cancerous tissues was significantly lower than that in adjacent non-cancerous tissues. EGOT was differentially expressed in a series of glioma cell lines, with its lowest level in high aggressive U251 and U87 cells. When EGOT was overexpressed by an expression plasmid, cell viability was significantly inhibited in U251 and U87 cells. Furthermore, with EGOT overexpression, the cell cycle was arrested at G0/G1 phase and consequently, cell apoptosis was significantly promoted along with the activities of caspase-3 and caspase-9. The migration abilities of EGOT-overexpressed cells were inhibited by 71.4% in U251 cells and by 69.5% in U87 cells. These data suggest that overexpression of EGOT inhibits cell proliferation and migration, and promotes cell apoptosis in glioma. Therefore, EGOT has potent anticancer activity and may function as a tumor suppressor in human glioma. IntroductionGlioma is the most common brain tumor, accounting for >50% of all brain tumors (1). According to a population-based investigation, the overall median survival duration for patients with malignant glioma is ~15 months (2). Despite significant advances in the therapeutic strategies of glioma, including neurosurgical approaches, chemotherapy and radiotherapy, the prognosis of patients with malignant glioma remains poor, primarily due to the fact that malignant glioma cells are highly aggressive and capable of infiltrating into adjacent normal brain tissue, leading to the eventual failure of complete surgical dissection of the tumor (3,4). Recurrence and resistance to chemotherapy are to be claimed two influential factors for prognosis (5,6). Therefore, developing novel strategies and elucidating innovative therapeutic agents for patients suffering from glioma are imperative and urgent tasks.Long noncoding RNAs (lncRNAs) are a type of RNAs that widely exist in the nuclei and cytoplasm of eukaryotic cells. These RNAs are non-protein coding and >200 nucleotides in length (7,8). With continuous advances in research approaches, research focused on lncRNAs has recently undergone a rapid expansion. LncRNAs are closely associated with tumor development (9,10) and particularly, have been implicated in glioma progression (11). For example, the lncRNA, MALAT1, has been demonstrated to have crucial roles in the cell proliferation, metastasis and apoptosis processes in glioma (12-14). MALAT1 was proposed as a tumor suppressor in glioma (14). Knockdown of MALAT1 increases the blood-tumor barrier permeability (15), and affects proliferation and ...

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Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling

Cited by 204 publications

References 36 publications

The Human RNA Surveillance Factor UPF1 Modulates Gastric Cancer Progression by Targeting Long Non-Coding RNA MALAT1

The Human RNA Surveillance Factor UPF1 Modulates Gastric Cancer Progression by Targeting Long Non-Coding RNA MALAT1

The Relationship Between MMP-2 -1306C>T and MMP-9 -1562C>T Polymorphisms and the Risk and Prognosis of T-Cell Acute Lymphoblastic Leukemia in a Chinese Population: A Case-Control Study

Long noncoding RNA eosinophil granule ontogeny transcript inhibits cell proliferation and migration and promotes cell apoptosis in human glioma

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