Tumor suppressive miR-196a is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

LI, YIFAN; Jin, Lü; CHEN, DUQUN; LIU, JIAJU; Su, Zhengming; Yang, Shangqi; Gui, Yaoting; Mao, Xiangming; Nie, Guohui; Lai, Yongqing

doi:10.3892/mmr.2016.5251

Cited by 12 publications

(7 citation statements)

References 36 publications

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“…We also demonstrated that miR-196a expression levels were significantly lower in specimens of EC tissue than in specimens of endometrial tissue obtained from AEH patients or normal control subjects. This supports previous studies that suggested miR-196a as a newly discovered promising biomarker for tumor progression (24)(25)(26)(27). However, its dysregulation in endometrioid endometrial carcinoma (EEC), revealed that miR-196a may also work synergistically with other miRNAs thought to be involved in various diseases (28).…”

Section: Discussionsupporting

confidence: 92%

Exploration of miR-1202 and miR-196a in human endometrial cancer based on high throughout gene screening analysis

Chen

Fan

et al. 2017

Oncology Reports

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Altered microRNA (miRNA) expression has been reported to participate in the pathogenesis of several human diseases, and particularly cancer. The present study examined the involvement of various miRNAs in the pathophysiology of endometrial cancer (EC) and atypical endometrial hyperplasia (AEH). We performed a high-throughput analysis of the miRNAs (miRNA microarray) found in samples of endometrial tissue obtained from 45 patients; among whom, 15 patients were diagnosed with EC, 15 patients were diagnosed with AEH, and the remainder were healthy donors. Next, we selected several miRNAs which exhibited at least a 2-fold difference in expression with a P<0.05 to validate these changes in 3 independent in vitro experiments that used real-time PCR analysis. Finally, miR-1202 and miR-196a were selected as target molecules whose effects on cell apoptosis, cell cycle changes, cell migratory and invasive abilities were investigated using flow cytometric and Transwell assays, respectively, after pre-treatment in vitro. After analyzing 125 miRNAs in a microarray assay, 6 miRNAs (3-high and 3-low expression) were further evaluated via paired comparison in all 3 groups. The validation test revealed a positive correlation between the microarray results and a high level of miR-1202 and a low level of miR-196a in the EC group, when compared with the AEH group. All of the data were normalized with data obtained from normal control donors. We found that either miR-1202 silencing or miR-196a overexpression affected AN3CA and HEC-1-A cells by increasing their apoptosis level and inducing G1 phase arrest while decreasing their migratory and invasive abilities. Inhibitors of miR-1202 and mimics of miR‑196a may exert a protective effect, suggesting that miR-1202 and miR‑196a may serve as biomarkers for evaluating the effectiveness of EC treatment.

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Section: Discussionsupporting

confidence: 92%

Exploration of miR-1202 and miR-196a in human endometrial cancer based on high throughout gene screening analysis

Chen

Fan

et al. 2017

Oncology Reports

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“…Finally, we provide the first demonstration that ANKHD1 mRNA and protein are significantly enhanced in human ccRCC when compared to control, and may play an important role, in human ccRCC. (6,(26)(27)(28)(29)(30)(31), a finding that correlates with the upregulation of ANKHD1, demonstrated here. Furthermore, given that single inhibition of miRNA-29a is sufficient to restore the proliferation of RCC cells lacking ANKHD1, we suggest that miR-29a-targeted genes are likely to be responsible for the ANKHD1-mediated proliferation of ccRCCs.…”

Section: Discussionsupporting

confidence: 71%

Ankyrin repeat and single KH domain 1 (ANKHD1) drives renal cancer cell proliferation via binding to and altering a subset of miRNAs

Fragiadaki

Zeidler

2018

Journal of Biological Chemistry

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“…Two recent studies have reported that several miRNAs are abnormally expressed in RCC and involved in RCC pathogenesis. For example, miR-196a and miR-149-5p are significantly downregulated in RCC and associated with proliferation, apoptosis, and migration (27,28). Das et al (29) reported that overexpression of miR-214 inhibited RCC proliferation by targeting the insulin-like growth factor-1 receptor.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-613 inhibits proliferation and invasion of renal cell carcinoma cells through targeting FZD7

Song

Nan

Wang

et al. 2017

Molecular Medicine Reports

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MicroRNAs (miRNAs) have emerged as critical regulators in cancer progression. miR‑613 has been reported as a tumor suppressor gene in many types of human cancers. However, the function of miR‑613 in renal cell carcinoma (RCC) remains unclear. In the present study, the authors aimed to detect the expression of miR‑613 and its function in RCC cell lines. miR‑613 was reported to be significantly downregulated RCC cell lines. Functional analyses demonstrated that overexpression of miR‑613 significantly decreased RCC cell proliferation and invasion. Bioinformatics analysis showed that Frizzled7 (FZD7) was a predicted target of miR‑613, which was verified by dual‑luciferase reporter assay, reverse transcription quantitative‑polymerase chain reaction and western blot analysis. Restoration of FZD7 significantly reversed the suppressive effects of miR‑613 on RCC cell proliferation and invasion. Taken together, the results of the present study indicated that miR‑613 functions as a tumor suppressor that inhibits RCC cell proliferation and invasion by targeting and inhibiting FZD7, providing novel insight into RCC pathogenesis and a potential therapeutic target for RCC.

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Tumor suppressive miR-196a is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Cited by 12 publications

References 36 publications

Exploration of miR-1202 and miR-196a in human endometrial cancer based on high throughout gene screening analysis

Exploration of miR-1202 and miR-196a in human endometrial cancer based on high throughout gene screening analysis

Ankyrin repeat and single KH domain 1 (ANKHD1) drives renal cancer cell proliferation via binding to and altering a subset of miRNAs

MicroRNA-613 inhibits proliferation and invasion of renal cell carcinoma cells through targeting FZD7

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