Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes

Ahmad, Akhlaq; Zhang, Weijie; Wu, Mingming; Tan, Sheng; Zhu, Tao

doi:10.1007/s13258-017-0624-6

Cited by 50 publications

(37 citation statements)

References 24 publications

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“…Other ndings of the present study indicated that has-miR-135 was downregulated only in the patients with a good response to the treatment. Some studies have shown that the induction of miR-135a expression in different types of cancers could suppress cell proliferation through target genes (c-MYC, STAT6, SMAD5, and BMPR2), and miR-135a has also been introduced as a potential predictor of treatment outcome in some cancers [44]. This study con rmed that these target genes are signi cant targets of has-miR-135 in achalasia (Table 4).…”

Section: Discussionsupporting

confidence: 61%

Alterations in Esophageal MicroRNAs Expression in Primary Esophageal Achalasia by Next-Generation Sequencing

Gholipour¹,

Mikaeli²,

Mowla³

et al. 2020

Preprint

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Background: Molecular knowledge regarding the primary esophageal achalasia is essential for early diagnosis and treatment of this neurodegenerative motility disorder. So, there is a need to find the main microRNAs (miRNAs) contributing to the mechanisms of achalasia.Methods: This study was conducted to determine some patterns of deregulated miRNAs in the achalasia. This case-control study was performed on 52 patients with achalasia and 50 non-achalasia controls. The miRNA expression profiling was conducted on the esophageal tissue samples obtained from the patients with achalasia and non-achalasia patients controls using the Next-Generation Sequencing (NGS). Differential expression of miRNAs was analyzed by the edgeR software. The selected dysregulated miRNAs were additionally confirmed using the quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). Potential target genes of the downregulated and upregulated miRNAs were also predicted to understand the putative role of the miRNAs in the development of the achalasia. Results: Totally, 15 miRNAs were identified that were significantly altered in the tissues of the patients with achalasia compared to the controls. Among them, three miRNAs including miR-133a-5p, miR-143-3p, and miR-6507-5p were upregulated. Also, six miRNAs including miR-215-5p, miR-216a-5p, miR-216b-5p, miR-217, miR-7641, and miR-194-5p were downregulated significantly. The predicted targets for the dysregulated miRNAs showed significant disease-associated pathways like neuronal cell apoptosis, neuromuscular balance, nerve growth factor signaling, and immune response regulation. Gene expression analysis confirmed significant downregulation of the hsa-miR-217 (p-value = 0.004) in the Lower Esophageal Sphincter (LES) of the patients with achalasia with significant enrichment in the myelination process ontology. The results of this study provide the first integrated miRNA expression profile in the achalasia using the NGS. Our findings introduced 15 candidate miRNAs as achalasia-associated non-coding RNAs and also confirmed the downregulation of the hsa-miR-217 in the achalasia disease. Conclusions: Our results may serve as a basis for more future functional studies to investigate the role of candidate miRNAs in the etiology of achalasia and their application in diagnosis and probably treatment of the disease.

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Section: Discussionsupporting

confidence: 61%

Alterations in Esophageal MicroRNAs Expression in Primary Esophageal Achalasia by Next-Generation Sequencing

Gholipour¹,

Mikaeli²,

Mowla³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Our results suggest that has-miR-135 is down-regulated only in good treatment response achalasia patients. Some studies showed that the induction of miR-135a expression in different types of cancers could suppress cell proliferation through target genes (c-MYC, STAT6, SMAD5, and BMPR2) and also miR-135a has been introduced as a potential predictor of treatment in some cancers [44]. We could show that these target genes are significant targets of has-miR-135 in achalasia.…”

Section: Deregulation Of Certain Individual Mirnas Compromises Immunementioning

confidence: 72%

Alterations in esophageal microRNAs expression in primary esophageal achalasia by next-generation sequencing

Gholipour

Mikaeli

Mowla

et al. 2020

Preprint

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Background The molecular knowledge of primary esophageal achalasia is essential for the early diagnosis and treatment of this neurodegenerative motility disorder. So it is substantial to find the main microRNAs (miRNAs), which are related to mechanisms of achalasia. Methods This study aimed to determine some patterns of deregulated miRNAs in achalasia. This case-control study was performed on 52 achalasia patients and 50 non-achalasia controls. The miRNA expression profiling was conducted on esophageal tissue samples from achalasia and non-achalasia patients, via next-generation sequencing (NGS). Differential expression of miRNAs was analyzed by edgeR software. The selected dysregulated miRNAs were additionally confirmed using RT-qPCR. Potential target genes of the down-regulated and up-regulated miRNAs were also predicted to understand the putative role of the miRNAs in achalasia. Results We identified 15 miRNAs that were significantly altered in the achalasia tissues compared to controls. Among these miRNAs, three; miR-133a-5p, miR-143-3p and miR-6507-5p were up-regulated. Also we found six miRNAs; miR-215-5p, miR-216a-5p, miR-216b-5p, miR-217, miR-7641 and miR-194-5p were down-regulated significantly. The predicted targets for the dysregulated miRNAs showed significant disease-associated pathways like neuron cell apoptosis, neuromuscular balance, neuron growth factor signaling and immune response regulation. Gene expression analysis confirmed significant downregulation of hsa-miR-217 (p-value =0.004) in LES of achalasia patients with significant enrichment in myelination process ontology. This study provides the first integrated miRNA expression profile using NGS in achalasia. Our findings introduced 15 candidate microRNAs as achalasia associated non-coding RNAs genes showing confirmed downregulation of the hsa-miR-217 in Achalasia disease. Conclusions Our results may serve as a basis for more future functional studies to discover the role of candidate miRNAs in the etiology of achalasia and their application in diagnosis and probably treatment.

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“…On the other hand, miRNA-135a showed a inhibitive role during the migration and invasion of lung cancer cells, due to targeting a transcription factor [19] . However, the functions and mechanisms of miRNA-135a during tumors are largely unknown [20][21][22][23] . Recent studies have demonstrated that miRNA-135a is up-regulated in CC cell lines SW480 and SW620, while in our study, the expression levels of miRNA-135a were significantly increased in CC tissues, which was in accordance with previous studies.…”

Section: Discussionmentioning

confidence: 99%

The expression and Clinical Significance of miRNA-135a and Bach1 in colorectal cancer

Wang

Zhang

Jiang

et al. 2020

Preprint

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AIM To explore the correlation between the expression of miRNA-135a and Bach1 in colorectal cancer tissue and the patient's clinical information. Methods 60 patients with colorectal carcinoma were treated as a control group. Real-time quantitative PCR assays and immunohistochemistry method were performed to detect the expression of miRNA-135a and Bach1 in 60 colorectal carcinoma and adjacent normal tissues, and the clinical and pathological classifications had also been investigated.The SPSS 19.00 software was used. All data represent mean±SD of three independent experiments. P<0.05 was considered statistically significant. Results miRNA-135a expression levels increased significantly in the colon cancer tissues compared with the non-tumor control tissues(P<0.01). miRNA-135a expression levels were higher in stage III/IV than in stage I/II colon cancer patients. The expression level of Bach1 in colorectal cancer was significantly lower(P<0.01). Bach1 and miRNA-135a were negatively correlated. Conclusions: The levels of miRNA-135a and Bach1 were opposite , the over-expression of miRNA-135a might decrease Bach1, which may be involved in the pathogenesis of colorectal cancer.

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Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes

Cited by 50 publications

References 24 publications

Alterations in Esophageal MicroRNAs Expression in Primary Esophageal Achalasia by Next-Generation Sequencing

Alterations in Esophageal MicroRNAs Expression in Primary Esophageal Achalasia by Next-Generation Sequencing

Alterations in esophageal microRNAs expression in primary esophageal achalasia by next-generation sequencing

The expression and Clinical Significance of miRNA-135a and Bach1 in colorectal cancer

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