Tumor suppressive role of microRNA-139-5p in bone marrow mesenchymal stem cells-derived extracellular vesicles in bladder cancer through regulation of the KIF3A/p21 axis

Xiang, Ying; Song, Tao; Niu, Chao; Wang, Ying

doi:10.1038/s41419-022-04936-0

Cited by 21 publications

(14 citation statements)

References 37 publications

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“…Extracellular vesicles play critical roles in the communications between cells. Highly expressed miR-139-5p, which is poorly present in bladder cancer, is confirmed in bone marrow mesenchymal stem cell-derived extracellular vesicles . Therefore, specific, sensitive, and convenient quantification of clinically relevant miRNAs is highly needed. − Moreover, since genes are always regulated by more than one miRNA, the multiplexing capability of a detection method is critical for practical utility.…”

mentioning

confidence: 99%

Quantification of Multiplex miRNAs by Mass Spectrometry with Duplex-Specific Nuclease-Mediated Amplification

Qian

Tang

2023

Anal. Chem.

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Early quantification of multiplex biomarkers such as microRNAs (miRNAs) is critical during disease pathologic development and therapy. To tackle challenges of low abundance and multiplexing, we herein report a mass-encoded biosensing approach with duplex-specific nuclease (DSN) mediated signal amplification. Magnetic Fe3O4 cores are coated with small gold nanoparticles (AuNPs), which are applied to achieve facile DNA immobilization subsequent separation. This biosensor integrates multiple mass reporters corresponding to different targets (five miRNAs as examples). Due to the excellent resolution of mass spectrometry, these targets can be successfully distinguished in a single spectrum. Wide detection ranges from 10 fM to 1 nM are achieved, and the limits of detection are estimated to be 10 fM. High selectivity is promised due to the enzyme activity of DSN, and practical application in human serum samples performs satisfactorily. The number of targets to be tested can be further expanded by designing different specific mass tags in theory. Therefore, the proposed method can be utilized as an important and valuable tool to quantify multiplex miRNAs for disease screening as well as biomedical investigations.

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confidence: 99%

Quantification of Multiplex miRNAs by Mass Spectrometry with Duplex-Specific Nuclease-Mediated Amplification

Qian

Tang

2023

Anal. Chem.

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“…The results have shown that the high expression of miR-20b-5p in tumor cells makes these cells more sensitive to radiation therapy, which has led to an increase in the efficiency of this treatment method [42]. Xiang et al showed that miR-139-5p in bone marrow mesenchymal stem cells (BMSCs) delays tumorigenesis and metastasis of bladder cancer cells in vivo by inactivating the p21 factor [43]. Also, microRNAs have an important regulatory role in diseases and are used as promising biomarkers for cancer diagnosis and prognosis.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of microRNA (miRNA) in cancer cells

Kanwal

Samarrai

Al-Zaidi³

et al. 2023

Cell. Mol. Biomed. Rep.

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MicroRNAs (miRNA) are a group of small non-coding RNAs that regulate gene expression at the RNA level. MicroRNAs have positive regulatory effects on protein translation processes and often induce their performance by binding to the 3'-UTR mRNA region. Also, microRNAs are involved in various cellular processes, including development, cell division, cell signaling, and cell growth, and generally play an effective role in the cell cycle and control of physiological processes and cell pathology. Several studies confirm that microRNAs play an important role in the initiation and progression of cancer, and many of them act as oncogenes and tumor suppressors. On the other hand, microRNAs are important stimulating factors that can act as biomarkers in the diagnosis and prognosis of various types of cancer, and in many cases, the occurrence of mutations in microRNAs and open-reading templates can lead to cancer. MicroRNAs also play an effective role in regulating gene expression. Biological studies have shown that about 30% of all genes and the majority of genetic pathways are regulated by microRNAs. In general, microRNAs and their target molecules are potential biological goals for primary screening, targeted treatment, and pharmaceutical resistance, and identifying them provides a clear prospect for a better understanding of the pathways leading to cancer. Article info

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“…These findings support the role of exosomes in cancer as well as the specific contributions in muscle invasive bladder cancer [ 82 , 85 , 86 ]. Additionally, to the above findings, there are several exosome biomarkers implicated in bladder cancer tumorigenesis [ 85 , 87 , 88 , 89 ]. These urine-based markers are characterized in Table 1 .…”

Section: Exosomes In Muscle Invasive Bladder Cancermentioning

confidence: 97%

Applications of Exosomes in Diagnosing Muscle Invasive Bladder Cancer

Walker

O’Malley

2022

Pharmaceutics

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Muscle Invasive Bladder Cancer (MIBC) is a subset of bladder cancer with a significant risk for metastases and death. It accounts for nearly 25% of bladder cancer diagnoses. A diagnostic work-up for MIBC is inclusive of urologic evaluation, radiographic imaging with a CT scan, urinalysis, and cystoscopy. These evaluations, especially cystoscopy, are invasive and carry the risk of secondary health concerns. Non-invasive diagnostics such as urine cytology are an attractive alternative currently being investigated to mitigate the requirement for cystoscopy. A pitfall in urine cytology is the lack of available options with high reliability, specificity, and sensitivity to malignant bladder cells. Exosomes are a novel biomarker source which could resolve some of the concerns with urine cytology, due to the high specificity as the surrogates of tumor cells. This review serves to define muscle invasive bladder cancer, current urine cytology methods, the role of exosomes in MIBC, and exosomes application as a diagnostic tool in MIBC. Urinary exosomes as the specific populations of extracellular vesicles could provide additional biomarkers with specificity and sensitivity to bladder malignancies, which are a consistent source of cellular information to direct clinicians for developing treatment strategies. Given its strong presence and differentiation ability between normal and cancerous cells, exosome-based urine cytology is highly promising in providing a perspective of a patient’s bladder cancer.

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Tumor suppressive role of microRNA-139-5p in bone marrow mesenchymal stem cells-derived extracellular vesicles in bladder cancer through regulation of the KIF3A/p21 axis

Cited by 21 publications

References 37 publications

Quantification of Multiplex miRNAs by Mass Spectrometry with Duplex-Specific Nuclease-Mediated Amplification

Quantification of Multiplex miRNAs by Mass Spectrometry with Duplex-Specific Nuclease-Mediated Amplification

Comprehensive analysis of microRNA (miRNA) in cancer cells

Applications of Exosomes in Diagnosing Muscle Invasive Bladder Cancer

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