Tumor suppressor function of miR‑483‑3p on breast cancer via targeting of the cyclin E1 gene

Huang, Xiaoxi; Jin, Ling

doi:10.3892/etm.2018.6504

Cited by 22 publications

(27 citation statements)

References 22 publications

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“…Overexpression of miR-647 promotes proliferation and migration of tumor cells by regulating expression levels of ANK2, FAK, MMP2, MMP12 and CD44 23. Previous studies have shown that miR-483-3p inhibits the progression of breast cancer by inhibiting CCNE1 expression 15. miR-483-3p reverses EMT and inhibits gefitinib resistance by directly targeting on integrin β3 through inhibiting downstream FAK/ERK pathway 24.…”

Section: Discussionmentioning

confidence: 99%

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<p>circZNF609 promotes the proliferation and migration of gastric cancer by sponging miR-483-3p and regulating CDK6</p>

Wei

Shao

et al. 2019

OTT

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ObjectiveTo explore the regulatory effects of circZNF609 on proliferative and migratory capacities of gastric cancer (GC) and its underlying mechanism.MethodsExpression level of circZNF609, CDK6 and miR-483-3p in GC tissues and cells were detected qRT-PCR verification. CCK-8 and transwell assay were conducted the cell viability and migratory capacities of GC cells. Dual luciferase assay was enrolled to confirm the interaction among circZNF609, CDK6 and miR-483-3p. Western blot was used to detect the protein level of CDK6.ResultsExpression levels of circZNF609 were higher in GC patients by qRT-PCR.GC patients with higher expression of circZNF609 were expected to have a higher TNM stage and lower 5-year survival than those with lower expression. ROC curves showed a well diagnostic value of circZNF609 in GC. Treatment of RNase R in GC cells downregulated the expression of ZNF609, whereas circZNF609 expression did not change. Furthermore, cytoplasmic expression of circZNF609 was higher than those of nuclear expression. Besides, biological experiments indicated that overexpression of circZNF609 promoted the proliferative and migratory capacities of GC cells. To demonstrate the underlying mechanism of circZNF609, we found that circZNF609 bound to miR-483-3p, which presented a lower expression in GC tissues than that of paracancerous tissues. Both circZNF609 and miR-483-3p could bind to Ago2, suggesting that circZNF609 may act as a sponge of miR-483-3p. In addition, the effect of overexpressed circZNF609 on cellular behaviors of GC cells were partly reversed by overexpression of miR-483-3p. Bioinformatics suggested that CDK6 has a potential binding site with miR-483-3p. The expression of CDK6 markedly increased in GC tissues and cells, which was negatively correlated with miR-483-3p expression. Dual-luciferase reporter gene results indicated that miR-483-3p could bind to the 3’-UTR of CDK6. Moreover, miR-483-3p downregulated CDK6 at both mRNA and protein levels. Overexpression of miR-483-3p inhibited proliferative and migratory capacities of GC cells, which were reversed by CDK6 overexpression.ConclusionIn summary, the expression of circZNF609 is upregulated in GC. CircZNF609 can be used as the sponge of miR-483-3p to regulate the expression level of CDK6, thus participating in the progression of GC by regulating the proliferative and migratory capacities of GC cells.

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Section: Discussionmentioning

confidence: 99%

“…circZNF609 shows an essential role in promoting myoblast proliferation 14. miR-483-3p inhibits the progression of breast cancer by inhibiting circCCNE1 expression 15. However, the roles of circZNF609 and miR-483-3p in GC have not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

<p>circZNF609 promotes the proliferation and migration of gastric cancer by sponging miR-483-3p and regulating CDK6</p>

Wei

Shao

et al. 2019

OTT

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“…This result is similar to that in other studies of cell lines (30), and a recent study has suggested that the interaction between RNA-binding ubiquitin E3 ligase (MEX3C) and adaptor-related protein complex 2(AP-2) may contribute to the enrichment phenomenon (31). miR-483-5p has been identified as an anti-tumor miRNA in breast cancer by targeting histone deacetylase 8 or Cyclin E1, which suggested the effects of miR-483-3p on cell growth and apoptosis (32,33).…”

Section: Discussionmentioning

confidence: 93%

Circulating Exosomal MicroRNAs as Diagnostic and Prognostic Biomarkers in Patients with Diffuse Large B-cell Lymphoma

Cao¹,

Jiang

Cao

et al. 2020

Preprint

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Background: Exosomal microRNAs (miRNAs) are potential biomarkers for a variety of tumors but have not yet been tested in diffuse large B-cell lymphoma (DLBCL). Here we sought to determine whether circulating exosomal miRNAs are differentially expressed in the blood of DLBCL patients compared with those in the blood of non-DLBCL patients with lymphoma and healthy subjects. In addition, we sought to explore whether circulating exosomal miRNAs could be used as prognostic predictors for DLBCL patients. Methods: Circulating exosomal miRNAs were isolated and used to perform miRNA expression profiling using quantitative reverse transcription polymerase chain reaction (qRT-PCR) based on Exiqon microarray from 10 DLBCL patients and 5 healthy subjects. Using qRT-PCR, miRNA candidates were first evaluated in the testing stage (24 DLBCL patients vs. 24 healthy subjects) and further confirmed in validation stage(99 DLBCL patients vs. 65 healthy subjects). Meanwhile, we also explored miRNAs concentrations in 29 non-DLBCL lymphoma cases, which were enrolled as concurrent control in this study. And lastly, relationships between miRNA level and patient outcomes including overall survival (OS) and progression-free survival (PFS) were studied. Results: Five circulating exosomal miRNAs (miR-379-5p, miR-135a-3p, miR-4476, miR-483-3p and miR-451a) were differently expressed in DLBCL patients compared with healthy controls. The 5-miRNA panel enabled us to predicted the probability of a diagnosis of DLBCL with an area under curve(AUC) of 0.863. Four circulating exosomal miRNAs (miR-379-5p, miR-135a-3p, miR-155-5p and miR-451a) were differently expressed between DLBCL patients and concurrent controls, the AUC of this 4-miRNA panel in distinguishing DLBCL patients from concurrent controls was 0.775. One miRNA, namely miR-451a, was significantly associated with both PFS and OS of DLBCL patients in the univariate analysis, and still statistically significant after adjusting for the International Prognostic Index(IPI) in the multivariate analysis. The combination of circulating miR-451a with IPI had a better predication for PFS and OS. Conclusions: Our study suggested subsets of circulating exosomal miRNAs could be useful noninvasive biomarkers for the diagnosis of DLBCL and the use of circulating exosomal miRNA improves the identification of patients with newly diagnosed DLBCL with poor outcomes.

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“…serves as an important target for tumor suppression/proliferation in BC [88]. For example, miRNAs, such as miR-497, miR-16, and miR-30c-2-3p, were reported to target and inhibit cell cycle regulator of G1-S transition, cyclin E1 leading to decreased cyclin E1 expression and suppression of proliferation by blocking BC cells from entering the S-phase of the cell cycle [89][90][91][92]. On the other hand, certain miRNAs, such as miR-483-3p, dysregulate the cell cycle transition by facilitating the formation of cyclin E1 and cyclin-dependent kinase CDK2 complex.…”

Section: Role Of Micrornas and Lncrna In Bcscsmentioning

confidence: 99%

“…On the other hand, certain miRNAs, such as miR-483-3p, dysregulate the cell cycle transition by facilitating the formation of cyclin E1 and cyclin-dependent kinase CDK2 complex. This leads to increased expression of cyclins, up-regulation of protein kinases and down-regulation of kinase inhibitors, thereby increasing BC cell viability and proliferation [92]. Similarly, overexpression of miR-1207-5p, has been associated with negative regulation of STAT2 expression and inactivation of cell cycle-dependent kinase inhibitors CDKN1A and CDKN1B thus promoting cell cycle progression in cancer cells [93].…”

Section: Role Of Micrornas and Lncrna In Bcscsmentioning

confidence: 99%

Non-Coding RNAs as Regulators and Markers for Targeting of Breast Cancer and Cancer Stem Cells

Prabhu

Raza

Karedath

et al. 2020

Cancers

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Breast cancer is regarded as a heterogeneous and complicated disease that remains the prime focus in the domain of public health concern. Next-generation sequencing technologies provided a new perspective dimension to non-coding RNAs, which were initially considered to be transcriptional noise or a product generated from erroneous transcription. Even though understanding of biological and molecular functions of noncoding RNA remains enigmatic, researchers have established the pivotal role of these RNAs in governing a plethora of biological phenomena that includes cancer-associated cellular processes such as proliferation, invasion, migration, apoptosis, and stemness. In addition to this, the transmission of microRNAs and long non-coding RNAs was identified as a source of communication to breast cancer cells either locally or systemically. The present review provides in-depth information with an aim at discovering the fundamental potential of non-coding RNAs, by providing knowledge of biogenesis and functional roles of micro RNA and long non-coding RNAs in breast cancer and breast cancer stem cells, as either oncogenic drivers or tumor suppressors. Furthermore, non-coding RNAs and their potential role as diagnostic and therapeutic moieties have also been summarized.

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Tumor suppressor function of miR‑483‑3p on breast cancer via targeting of the cyclin E1 gene

Cited by 22 publications

References 22 publications

<p>circZNF609 promotes the proliferation and migration of gastric cancer by sponging miR-483-3p and regulating CDK6</p>

<p>circZNF609 promotes the proliferation and migration of gastric cancer by sponging miR-483-3p and regulating CDK6</p>

Circulating Exosomal MicroRNAs as Diagnostic and Prognostic Biomarkers in Patients with Diffuse Large B-cell Lymphoma

Non-Coding RNAs as Regulators and Markers for Targeting of Breast Cancer and Cancer Stem Cells

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