Tumor suppressor miR‐192‐5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer

Abstract

Cervical cancer is the fourth highest mortality cancer among women worldwide.Many researchers have discovered the major anticancer role of miR-192-5p.However, no study has revealed the effect of miR-192-5p on cervical cancer and its molecular mechanism. Therefore, in this study, we aimed to explore the role of miR-192-5p in proliferation, invasion of cervical cancer, and its regulatory mechanism. Firstly, the expression level of miR-192-5p was examined by real-time quantitative polymerase chain reaction. Cell counting kit-8 analysis was applied to detect the proliferation of transfected Caski and SiHa cells. Flow cytometry assay was applied to detect the apoptosis of transfected Caski and SiHa cells. Our result showed that miR-192-5p restrained cervical cancer cell proliferation and induced apoptosis. Then we employed wound healing and transwell assays to analyze the migration and invasion abilities of Caski and SiHa cells in vitro. The results showed that miR-192-5p had an inhibitory effect on cervical cancer migration and invasion. The results of in vivo experiment demonstrated that miR-192-5p also inhibited tumor development in nude mice. We further detected that the binding of transient receptor potential melastatin-subfamily member 7 (TRPM7) to miR-192-5p using bioinformatic methods and dual-luciferase reporter assay. Finally, we found that TRPM7 overexpression reversed the inhibitory effects of miR-192-5p on proliferation, migration, and invasion on cervical cancer cells. In conclusion, the findings of the present study revealed that miR-192-5p performs an inhibitory role in cervical cancer proliferation and invasion by targeting TRPM7.

MicroRNA-3137/Nuclear Protein Transcription Regulator 1 Axis Regulates Cervical Cancer Cells Proliferation Apoptosis and Autophagy by PI3K/AKT/mTOR pathway

“…MiRNAs act on post-transcriptionally through binding to mRNA 3'UTR and thereby modulating mRNA expression and this model has been widely applied in exploring the molecular mechanism of miRNAs in cancers, CC included [31] . For instance, miR-192-5p targets TRPM7 to hinder proliferation in CC cells [32] . MiR-499a accelerates the progression and chemo resistance of CC cells by binding to SOX6 [33] .…”

Up‐regulation of miR‐192‐5p inhibits the ELAVL1/PI3Kδ axis and attenuates microvascular endothelial cell proliferation, migration and angiogenesis in diabetic retinopathy

“…MiR‐192‐5p has been reported to bind to targets in a variety of diseases. For example, miR‐192‐5p could target TRPM7, thereby inhibiting cervical cancer proliferation and invasion 23 . Overexpressed miR‐192‐5p rendered gastric cancer cells more sensitive to cisplatin 24 .…”

“…For example, miR-192-5p could target TRPM7, thereby inhibiting cervical cancer proliferation and invasion. 23 Overexpressed miR-192-5p rendered gastric cancer cells more sensitive to cisplatin. 24 In our project, miR-192-5p could target ELAVL1 and negatively regulate its expression.…”

OIP5-AS1/CD147/TRPM7 axis promotes gastric cancer metastasis by regulating apoptosis related PI3K-Akt signaling

“…Ca 2+ and Mg 2+ play an important role in countless cell processes. Among ion channels, transient receptor potential melastatin (TRPM) channels 6 and 7 have similar permeability to the main divalent cations Ca 2+ and Mg 2+ ( 13 ). Studies have shown that the activation of TRPM7 channel is very important for human head and neck cancer cells ( 14 , 15 ).…”