Tumor targeting by lentiviral vectors combined with magnetic nanoparticles in mice

Borroni, Ester; Miola, Marta; Ferraris, Sara; Ricci, Giulia; Rožman, Kristina Žužek; Kostevšek, Nina; Catizone, Angela; Rimondini, Lia; Prat, María; Verné, Enrica; Follenzi, Antonia

doi:10.1016/j.actbio.2017.07.007

Cited by 37 publications

(38 citation statements)

References 60 publications

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“…The superparamagnetic behavior of both, Fe 3 O 4 and SiO 2 ‐Fe 3 O 4 NPs, were confirmed as the absence of any remanent magnetization and coercivity at room temperature . Furthermore, colloidal stability of NPs after 48 hours was confirmed with the suspension stability test with and without the presence of the external magnetic field, as shown in Figure .…”

Section: Discussionmentioning

confidence: 63%

“…The magnetic properties of the 2 kinds of SPIONs were investigated using a Vibrating Samples Magnetometer (VSM ‐Lakeshore). Both Fe 3 O 4 and SiO 2 ‐Fe 3 O 4 NPs exhibited a superparamagnetic behavior since the hysteresis cycles (reported in Ref) revealed no evidence of remanent magnetization and coercivity. The saturation magnetization (Ms) of uncoated Fe 3 O 4 was 58 emu/g, while silica coating reduced Ms at 31 emu/g (Table ).…”

Section: Resultsmentioning

confidence: 86%

“…Iron oxide nanoparticles (Fe 3 O 4 NPs) and silica‐coated iron oxide nanoparticles (SiO 2 ‐Fe 3 O 4 NPs) were synthesized as reported in Refs and suspended in H 2 O. All reagents were purchased from Sigma‐Aldrich, unless otherwise specified in the text.…”

Section: Methodsmentioning

confidence: 99%

“…Muzio et al investigated the biological responses (viability and internalization) triggered by Fe 3 O 4 and Fe 3 O 4 ‐SiO 2 NPs (unfunctionalized or conjugated with linoleic acid) in mouse breast cancer 4T1 cells and pancreatic islet endothelial MS1 cells (a nontumorigenic sinusoidal endothelial cell line derived from pancreas). Finally, in MS1 cells Borroni et al demonstrated that slight or no reduction in cell viability was induced by exposure to Fe 3 O 4 or Fe 3 O 4 ‐SiO 2 NPs.…”

Section: Introductionmentioning

confidence: 99%

“…Some of the authors of this work have already assessed the biocompatibility of Fe 3 O 4 NPs and SiO 2 ‐Fe 3 O 4 NPs on endothelial cells, their effect on mouse breast cancer 4T1 cells and on pancreatic islet endothelial MS1 cells . The NPs tumor targeting ability was also assessed by coupling with Lentiviral Vectors . Catalano et al performed direct and indirect cytotoxicity assays, ROS generation, and apoptosis enzymes activation tests, demonstrating that both Fe 3 O 4 and Fe 3 O 4 ‐SiO 2 NPs are cytocompatible, in a concentration‐dependent mode, in a mouse endothelial cell line.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and characterization of silica‐coated superparamagnetic iron oxide nanoparticles and interaction with pancreatic cancer cells

Multari

Miola

Ferraris

et al. 2018

Int J Applied Ceramic Tech

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The aim of the study is to investigate the relationship between the physico-chemical properties of superparamagnetic iron oxide nanoparticles (SPIONs) and their cytotoxicity profile in light of their potential biomedical application as nanocarriers for pancreatic cancer treatment. Two types of SPIONs were tested: magnetite nanoparticles (Fe 3 O 4 NPs) and silica-coated magnetite nanoparticles (SiO 2 -Fe 3 O 4 NPs). The physico-chemical properties of the 2 SPIONs were characterized by means of Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Spectrometry (EDS), and Selected Area Electron Diffraction (SAED). Their magnetic properties were quantified as magnetization saturation (Ms) and Remanence. The colloidal stability was investigated by Isoelectric Point Measurements and sedimentation tests. Finally, in vitro characterizations were performed to quantify the half maximal lethal concentration (LC 50 ), by means of High Content Screening Analysis (HCSA), Flow cytometry (FC), and Laser Scanning Confocal Microscopy (LSCM). The obtained NPs present a spherical shape and a dimension between 10 and 20 nm, a superparamagnetic behavior and surface charge in agreement with their surface chemistry. The in vitro tests demonstrate that both NPs induce similar levels of cytotoxicity in a PANC-1 cell model and were internalized, with SiO 2 -Fe 3 O 4 NPs associated to a slightly higher cellular internalization, probably due to their higher dispersability.

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Section: Discussionmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Synthesis and characterization of silica‐coated superparamagnetic iron oxide nanoparticles and interaction with pancreatic cancer cells

Multari

Miola

Ferraris

et al. 2018

Int J Applied Ceramic Tech

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Development of Super‐Paramagnetic Iron Oxide Nanoparticle‐Coated Melt Electrowritten Scaffolds for Biomedical Applications

Unalan,

Occhipinti,

Miola

et al. 2023

Macromolecular Bioscience

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Polycaprolactone (PCL) is usually the material chosen for melt electrowriting (MEW) due to its biocompatibility, mechanical strength, and melt processability. This work first investigates the effect of different processing parameters to obtain optimum PCL‐MEW scaffolds. Secondly, to increase PCL`s hydrophilicity and cell affinity, and to enable coating with superparamagnetic iron oxide nanoparticles (SPIONs) and silica‐coated‐SPIONs (Si‐SPIONs), the scaffolds are modified with alkaline surface treatment. Finally, SPIONs and Si‐SPIONs are successfully coated on MEW scaffolds. Results show that reproducible scaffolds are fabricated. Additionally, the alkaline treatment does not change the three‐dimensional morphology of scaffolds while reducing fiber diameter. Furthermore, SEM images and ATR‐FTIR results confirmed that SPIONs and Si‐SPIONs‐were coated on scaffolds. A cytocompatibility assay showed a non‐toxic effect on MG‐63 osteoblast‐like cells in all scaffolds. Additionally, higher MC3T3‐E1 pre‐osteoblastic cell adhesion efficiency and proliferation are achieved for the alkaline‐treated scaffolds and SPIONs/Si‐SPIONs‐coated scaffolds. All samples demonstrated the ability to generate heat, useful for hyperthermia‐treatment, when subjected to an alternating magnetic field. Overall, the findings suggest that the strategy of coating PCL‐MEW scaffolds with SPIONs/Si‐SPIONs has the potential to improve scaffold performance for biomedical applications, especially for producing magnetically responsive MEW scaffolds.

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Hybrid Silica‐Coated Nanomaterials: Pioneering Breakthroughs in Biomedical Technology

Liao,

Elkalla,

Khizar

et al. 2024

Polymers for Advanced Techs

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The field of biomedicine has extensively studied silica‐coated organic/inorganic nanoparticles due to their remarkable properties, such as biocompatibility, chemical stability, ease of functionalization, and thermal stability. Silica has been utilized to coat or encapsulate organic/inorganic nanoparticles, providing protection against aggregation and acid leaching while improving cytocompatibility. This review provides an overview of the manufacturing methods for silica nanoparticles, with a specific focus on surface strategies for core template nanomaterials (both hard and soft templates). These strategies involve the use of chemicals or biomolecules to guide nucleation and growth of silica at the interface of two phases. Furthermore, recent advancements in silica‐coated nanoparticles are discussed, emphasizing their applications in various areas such as bioimaging, drug delivery, biosensors, phototherapy, and hyperthermia.

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Tumor targeting by lentiviral vectors combined with magnetic nanoparticles in mice

Cited by 37 publications

References 60 publications

Synthesis and characterization of silica‐coated superparamagnetic iron oxide nanoparticles and interaction with pancreatic cancer cells

Synthesis and characterization of silica‐coated superparamagnetic iron oxide nanoparticles and interaction with pancreatic cancer cells

Development of Super‐Paramagnetic Iron Oxide Nanoparticle‐Coated Melt Electrowritten Scaffolds for Biomedical Applications

Hybrid Silica‐Coated Nanomaterials: Pioneering Breakthroughs in Biomedical Technology

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