Tumor Vascularity Assessed By Magnetic Resonance Imaging and Intravital Microscopy Imaging

Gaustad, Jon Vidar; Brurberg, Kjetil Gundro; Simonsen, Trude G.; Mollatt, Camilla; Rofstad, Einar K.

doi:10.1593/neo.08162

Cited by 66 publications

(70 citation statements)

References 30 publications

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“…EXTRACTING INFORMATION about the architecture of microvascular beds is extremely important for understanding tissue oxygenation and metabolism of well-perfused organs (brain (1,2), myocardium (3), skeletal muscle (4)), as well as for investigating numerous pathologies such as vascular dementia (5) or tumor angiogenesis (6,7). While intravital microscopy can probe the microcirculation in superficial tissues (8), magnetic resonance imaging (MRI) can probe functionally relevant properties of the microcirculation of living tissues deep within the body (6) using various contrast agents or signal mechanisms.…”

mentioning

confidence: 99%

Intravoxel partially coherent motion technique: Characterization of the anisotropy of skeletal muscle microvasculature

Karampinos

King

Sutton

et al. 2010

Magnetic Resonance Imaging

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Purpose: To propose a reformulation of the intravoxel incoherent motion (IVIM) technique exploiting the low bvalue diffusion-weighted imaging regime that can characterize microcirculation of tissues perfused with partially coherent blood flow. Materials and Methods:The new methodology, termed intravoxel partially coherent motion (IVPCM) technique, is suitable for probing microcirculation in tissues with ordered microvasculature, such as skeletal muscle. We employ a subvoxel model utilizing a randomly oriented bundle of straight vessels whose orientation statistics are characterized by a Fisher axial distribution with concentration parameter K quantifying the anisotropy of the distribution (K ¼ 0 indicates isotropic capillary orientation). The methodology is first validated with a proof-of-principle phantom experiment and is then applied to analyze the microvasculature of human calf muscle at rest. Results:The microcirculatory part of the diffusionweighted signal at b < 200 s/mm 2 is anisotropic. The variation of the diffusion-weighted signal with b-value exhibits stronger deviation from the expected monoexponential decay when the diffusion encoding gradient is applied parallel to the mean myofiber direction in the calf muscle of three healthy volunteers. The application of the model to data from the medial gastrocnemius and the soleus of the three volunteers gives results within the expected range for the mean microvascular volume fraction, the mean microflow velocity, and the parameter K. Conclusion:The proposed methodology has the capability of characterizing the anisotropy of the capillary network in vivo in a manner analogous to the capability of high b-value diffusion to characterize the anisotropy of muscle fibers.

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confidence: 99%

Intravoxel partially coherent motion technique: Characterization of the anisotropy of skeletal muscle microvasculature

Karampinos

King

Sutton

et al. 2010

Magnetic Resonance Imaging

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“…Positive correlations were found between and EESF obtained by invasive imaging. Gaustad et al [25] investigated whether Gd-DTPA-based DCE-MRI can be a useful method for characterizing vascularity of tumors by comparing the images of ⋅ as obtained by Kety analysis with Blood Supply Time (BST) images (i.e., images of the time from when arterial blood enters a tumor through the supplying artery until it reaches a vessel segment within the tumor) and morphologic images of the microvascular network which were produced by intravital microscopy. They found that the ⋅ images mirrored the morphology (microvascular density) and the function (BST) of the microvascular networks well.…”

Section: Discussionmentioning

confidence: 99%

Validation of Microcirculatory Parameters Derived from the Standard Two-Compartment Model with Murine Xenografts Model

Hartono

Thng

Ong

et al. 2015

Journal of Cancer Research

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The purpose of this study was to validate DCE-MRI parameters such as blood flow (F), permeability surface area product (PS), fractional intravascular space (V 1 ), and fractional extracellular extravascular space (V 2 ), obtained using a standard two-compartment model against other established analysis methods and histological indices. DCE-MRI datasets of 28 mice implanted with various human cancer xenografts were acquired and analyzed. Statistically significant correlations were found between the parameters derived from the standard two-compartment model (V 1 , V 2 , F, and PS) with the histological markers of intravascular and interstitial space and with the corresponding flow and permeability estimates obtained by the initial slope method and Patlak plot, respectively.

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“…Besides, nonmetallic chambers composed of polymer materials have been fabricated, which can be applied to studies employing ionising or non-ionising radiation in animal exposure experiments without inducing thermal effects (Ushiyama et al, 2004). Moreover, they are compatible for magnetic resonance imaging (MRI) (Gaustad et al, 2008;Erten et al, 2010).…”

Section: Characteristics Of the Dorsal Skinfold Chamber Modelmentioning

confidence: 99%

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Laschke

Vollmar²,

Menger³

2011

eCM

146

128

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The implantation of biomaterials into the human body has become an indispensable part of almost all fi elds of modern medicine. Accordingly, there is an increasing need for appropriate approaches, which can be used to evaluate the suitability of different biomaterials for distinct clinical indications. The dorsal skinfold chamber is a sophisticated experimental model, which has been proven to be extremely valuable for the systematic in vivo analysis of the dynamic interaction of small biomaterial implants with the surrounding host tissue in rats, hamsters and mice. By means of intravital fl uorescence microscopy, this chronic model allows for repeated analyses of various cellular, molecular and microvascular mechanisms, which are involved in the early infl ammatory and angiogenic host tissue response to biomaterials during the initial 2-3 weeks after implantation. Therefore, the dorsal skinfold chamber has been broadly used during the last two decades to assess the in vivo performance of prosthetic vascular grafts, metallic implants, surgical meshes, bone substitutes, scaffolds for tissue engineering, as well as for locally or systemically applied drug delivery systems. These studies have contributed to identify basic material properties determining the biocompatibility of the implants and vascular ingrowth into their surface or internal structures. Thus, the dorsal skinfold chamber model does not only provide deep insights into the complex interactions of biomaterials with the surrounding soft tissues of the host but also represents an important tool for the future development of novel biomaterials aiming at an optimisation of their biofunctionality in clinical practice.

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Tumor Vascularity Assessed By Magnetic Resonance Imaging and Intravital Microscopy Imaging

Cited by 66 publications

References 30 publications

Intravoxel partially coherent motion technique: Characterization of the anisotropy of skeletal muscle microvasculature

Intravoxel partially coherent motion technique: Characterization of the anisotropy of skeletal muscle microvasculature

Validation of Microcirculatory Parameters Derived from the Standard Two-Compartment Model with Murine Xenografts Model

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

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