Tumorigenic poxviruses: Fine analysis of the recombination junctions in malignant rabbit fibroma virus, a recombinant between shope fibroma virus and myxoma virus

Upton, Chris; Macen, Joanne; Maranchuk, Rob; DeLange, A. M.; McFadden, Grant

doi:10.1016/0042-6822(88)90164-x

Cited by 43 publications

(23 citation statements)

References 24 publications

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“…DNA recombination is known to occur efficiently between viral and transfected plasmid DNA [9], and recombinant viruses have been recognized [8,29]. However, we were surprised by the large number of indel patterns that did not match the phylogenetic tree, for example, deletion-70 (Electronic supplementary material 3), which is present in the 4 Acambis 2000-like genomes, Ankara-CVA and LC16m0/8 and its absence from the other VACV strains, notably the 4 MVA isolates and Lister.…”

Section: Discussionmentioning

confidence: 99%

Characterization of indels in poxvirus genomes

Coulson¹,

Upton²

2010

Virus Genes

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By comparing sets of variola virus (VARV) genomes and sets of vaccinia virus (VACV) genomes, it was found that the insertion and deletion of small pieces of DNA (3-25 nucleotides) were common events among these poxviruses. Insertion events were characterized by the creation of tandem direct repeats, whereas the deletion events generally took place between two direct repeats that were separated by a few nucleotides. A number of the VARV and VACV indels clearly did not fit the expected phylogenetic tree patterns. Some of these were found to be the result of coincident events, but others, in VACV, suggest recombination among the VACV genomes. Such recombination would make the construction of phylogenetic trees problematic. The growth of VACV under artificial conditions and at high multiplicities does not select against these deletions.

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Section: Discussionmentioning

confidence: 99%

Characterization of indels in poxvirus genomes

Coulson¹,

Upton²

2010

Virus Genes

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“…Whether this is the result of additional highly localized recombination or selective events is unclear and merits further investigation. A similarly complex laboratory recombination event occurred between RFV and a South American MYXV to form malignant rabbit virus (MRV) (42,43).…”

Section: Resultsmentioning

confidence: 99%

Comparative Analysis of the Complete Genome Sequence of the California MSW Strain of Myxoma Virus Reveals Potential Host Adaptations

Kerr

Rogers

Fitch

et al. 2013

J Virol

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“…Peripheral regions of the DNA, within and near the inverted terminal repeats (ITR) at both sides of the genome, encode nonessential factors that contribute to the modulation of the host response to infection [29, 31, 32]. Two genes present near the right ITR, M148R and M149R, have functions that are still speculative.…”

Section: Introductionmentioning

confidence: 99%

M148R and M149R are two virulence factors for myxoma virus pathogenesis in the European rabbit

et al. 2008

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Myxoma virus (MYXV), a member of the Poxviridae family, is the agent responsible for myxomatosis, a fatal disease in the European rabbit (Oryctolagus cuniculus). MYXV has a linear double-stranded DNA genome that encodes several factors important for evasion from the host immune system. Among them, four ankyrin (ANK) repeat proteins were identified: M148R, M149R, M150R and M-T5. To date, only M150R and M-T5 were studied and characterized as critical virulence factors. This article presents the first characterization of M148R and M149R. Green Fluorescent Protein (GFP) fusions allowed us to localize them in a viral context. Whereas M149R is only cytoplasmic, interestingly, M148R is in part located in the nucleolus, a unique feature for an ANK repeat poxviral protein. In order to evaluate their implication in viral pathogenicity, targeted M148R, M149R, or both deletions were constructed in the wild type T1 strain of myxoma virus. In vitro infection of rabbit and primate cultured cells as well as primary rabbit cells allowed us to conclude that M148R and M149R are not likely to be implicated in cell tropism or host range functions. However, in vivo experiments revealed that they are virulence factors since after infection of European rabbits with mutant viruses, a delay in the onset of clinical signs, an increase of survival time and a dramatic decrease in mortality rate were observed. Moreover, histological analysis suggests that M148R plays a role in the subversion of host inflammatory response by MYXV.

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Tumorigenic poxviruses: Fine analysis of the recombination junctions in malignant rabbit fibroma virus, a recombinant between shope fibroma virus and myxoma virus

Cited by 43 publications

References 24 publications

Characterization of indels in poxvirus genomes

Characterization of indels in poxvirus genomes

Comparative Analysis of the Complete Genome Sequence of the California MSW Strain of Myxoma Virus Reveals Potential Host Adaptations

M148R and M149R are two virulence factors for myxoma virus pathogenesis in the European rabbit

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