Tumour angiogenesis and tumour cell proliferation as prognostic indicators in gastric carcinoma

Maeda, Kiyoshi; Chung, Yong‐Suk; Takatsuka, Satoshi; Ogawa, Yoshinari; Onoda, Naoyoshi; Sawada, Tetsuji; Kato, Yasuyuki; Nitta, Atsunori; Arimoto, Yuichi; Kondo, Yasuyuki

doi:10.1038/bjc.1995.331

Cited by 159 publications

(107 citation statements)

References 13 publications

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“…Additionally, the mean number of VEGF-immunopositive vessels was determined based on a method adapted from Maeda et al (10). Tissue sections were examined by light microscopy at ×40 magnification and five areas showing the highest vascularization were identified subjectively.…”

Section: Evaluation Of Immunostaining and Statistical Analysismentioning

confidence: 99%

Immunohistochemical expression of MMP-9 and VEGF in squamous cell carcinoma of the tongue

et al. 2012

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Section: Evaluation Of Immunostaining and Statistical Analysismentioning

confidence: 99%

Immunohistochemical expression of MMP-9 and VEGF in squamous cell carcinoma of the tongue

et al. 2012

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“…Ketoprofen is listed as a drug that would potentially target multiple components of tumor microenvironment (angiogenesis, inflammation, proliferative activity), however, the results of the effects of ketoprofen on a number of clinical and experimental protocols are rather contradictory and/or inconsistent (Marjanovic´ et al, 2007;Kamei et al, 2008). Thus, understanding the mechanisms of ketoprofen action may uncover strategies to enhance the efficacy of this important therapeutic agent.…”

Section: Discussionmentioning

confidence: 99%

“…The method described by Maeda et al (1995), was used to count microvascular density (MVD) in hot spot areas (areas with higher number of blood vessels).…”

Section: Methodsmentioning

confidence: 99%

“…Kamei et al (2008) found that topical administration of 286 mg daily of ketoprofen inhibited tumor growth of osteosarcoma and lung metastases. Marjanovic et al (2007) showed that the antiproliferative ketoprofen possessed low capacity when compared with the unchanged drug, possibly by increasing the cytotoxic activity and increased absorption of the cells.…”

Section: Arqmentioning

confidence: 99%

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Subcutaneous administration of ketoprofen delays Ehrlich solid tumor growth in mice

Souza

Auler

Reis

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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Ketoprofen, a nonsteroidal anti-inflammatory drug (NSAID) has proven to exert anti-inflammatory, antiproliferative and anti-angiogenic activities in both neoplastic and non-neoplastic conditions. We investigated the effects of this compound on tumor development in Swiss mice previously inoculated with Ehrlich tumor cells. To carry out this study the solid tumor was obtained from cells of the ascites fluid of Ehrlich tumor resuspended in physiological saline to give 2.5x10 6 cells in 0.05mL. After tumor inoculation, the animals were separated into two groups (n = 10). The animals treated with ketoprofen 0.1µg/100µL/animal were injected intraperitoneally at intervals of 24h for 10 consecutive days. Animals from the control group received saline. At the end of the experiment the mice were killed and the tumor removed. We analyzed tumor growth, histomorphological and immunohistochemical characteristics for CDC47 (cellular proliferation marker) and for CD31 (blood vessel marker). Animals treated with the ketoprofen 0.1µg/100µL/animal showed lower tumor growth. The treatment did not significantly influence the size of the areas of cancer, inflammation, necrosis and hemorrhage. Moreover, lower rates of tumor cell proliferation were observed in animals treated with ketoprofen compared with the untreated control group. The participation of ketoprofen in controlling tumor malignant cell proliferation would open prospects for its use in clinical and antineoplasic therapy.Keywords: inflammation, cell proliferation, Ketoprofen, neoplasm RESUMO O cetoprofeno é uma droga anti-inflamatória não esteroidal (NSAID), que exerce efeito anti-inflamatório, antiproliferativo e antitumoral em condições neoplásicas e não neoplásicas. Foram investigados os efeitos desse composto sobre o desenvolvimento tumoral de camundongos

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“…The blood vessels can also spill tumour cells throughout the host, associating angiogenesis directly with the formation of metastases [1,2]. Given this critical role in tumour enlargement and metastatic potential, it is not surprising that prognosis is directly related to the degree of intratumoural vascularisation in many tumours [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Determination of Tumour Prognosis Based on Angiogenesis-related Vascular Patterns Measured by Fractal and Syntactic Structure Analysis

et al. 2004

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ABSTRACT:Aims: Intratumoural micro-vessel density (IMD) has recently been shown to be a valuable prognostic tool in many tumours. Yet, IMD does not take into account the spatial arrangement of the vessels, therefore only partly reflecting the angiogenic situation. In order to describe contextual vascular relationships more accurately, we have used fractal and syntactic structure analysis (SSA) based on computerised image processing to quantify micro-vascular hot spots. Materials and methods:The parametric performance in prediction of patients' outcome was evaluated by univariate analysis and compared with manually obtained IMDs, whereas an automated K-nearest-neighbour (KNN) classifier searched most discriminative parametric combinations. The method is based on analysis of vascular 'hot-spots' of paraffin-embedded tissue sections of invasive cervical carcinoma, colorectal carcinoma and malignant mesothelioma. Results: For all three cancers, prediction of prognosis based on SSA yielded in general much higher recognition scores compared with IMD or fractal dimension. Survival of cervical carcinoma was mostly correlated with clinical data, with the vascular permeation being the only parameter with independent value. Prognosis of colorectal carcinoma is best described by SSA, completed with IMD, indicating an inverse correlation of survival time with a more irregular pattern and a slight increase in vessel number. For mesothelioma, we found a strong correlation with SSA and patients' outcome, with two SSA-parameters having independent prognostic value. Conclusions: The more accurate angiogenic description obtained with SSA may be useful for further exploitation as a prognosticator in a general diagnostic pathology service.

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Tumour angiogenesis and tumour cell proliferation as prognostic indicators in gastric carcinoma

Cited by 159 publications

References 13 publications

Immunohistochemical expression of MMP-9 and VEGF in squamous cell carcinoma of the tongue

Immunohistochemical expression of MMP-9 and VEGF in squamous cell carcinoma of the tongue

Subcutaneous administration of ketoprofen delays Ehrlich solid tumor growth in mice

Determination of Tumour Prognosis Based on Angiogenesis-related Vascular Patterns Measured by Fractal and Syntactic Structure Analysis

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