Tumour Immunology: Alternative Perspectives

Seljelid, Rolf

doi:10.1046/j.1365-3083.1997.d01-160.x

Cited by 7 publications

(7 citation statements)

References 47 publications

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“…We isolated lymphocytes from both HCC and CHM , which were expanded (Seljelid, 1997) in vitro to generate sufficient numbers for functional studies. We studied their migratory and adhesive interactions with vitronectin.…”

Section: Discussionmentioning

confidence: 99%

Vitronectin in human hepatic tumours contributes to the recruitment of lymphocytes in an αvβ3-independent manner

et al. 2006

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The degree of lymphocyte infiltration is a prognostic factor in liver cancer, but to date the mechanisms by which lymphocytes infiltrate into and are retained in hepatic tumours are poorly understood. We hypothesised that the extracellular matrix glycoprotein vitronectin, a major component of the stroma of hepatic tumours, might play a role in the recruitment and retention of tumourinfiltrating lymphocytes (TIL). Thus, we investigated the ability of vitronectin to support migration and adhesion of TIL isolated from hepatocellular carcinoma and colorectal hepatic metastases. Soluble vitronectin-induced dose-dependent migration of TIL in in vitro chemotaxis and haptotaxis assays and vitronectin in tissue sections was able to support TIL adhesion to tumour stroma. Neither adhesion nor migration was inhibited by a function blocking mAb against the major vitronectin receptor avb3 and we were unable to detect avb3 on TIL in vitro or in vivo on tumour tissue. However, TIL did express high levels of urokinase-type plasminogen activator receptor (uPAR) and inhibitory antibodies and amiloride both significantly inhibited TIL adhesion to vitronectin and reduced transendothelial migration of lymphocytes across liver endothelium in vitro. Thus, we provide evidence that vitronectin in liver tumours can support the recruitment and retention of effector lymphocytes by an uPAR-dependent mechanism.

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Section: Discussionmentioning

confidence: 99%

Vitronectin in human hepatic tumours contributes to the recruitment of lymphocytes in an αvβ3-independent manner

et al. 2006

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“…There are many factors involved in the tolerance of cancer cell growth by the immune system (reviewed in (62,66)). Active suppression of immunity by secretion of immunoregulatory cytokines including TGFb and IL-10, and the downregulation of TAP and MHC molecules of immune escape variants, are a few of the many aspects of cancer cell growth that can lead to immune ignorance of cancer.…”

Section: Absence Of Danger In Immune Ignorancementioning

confidence: 99%

Dendritic cell activation by danger and antigen‐specific T‐cell signalling

McLellan

Bröcker

Kämpgen

2000

Experimental Dermatology

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Recent transplantation, animal and in vitro studies suggest a dependence of some immune reactions on tissue damage. Although many factors involved in enhancing immune responses through tissue damage have yet to be identified, recent data suggests that one of the targets of these cellular stress factors is the bone marrow derived dendritic cell (DC). DC are potent initiators of primary immune responses and hold the key to immune reactions through their ability to sense changes in their local environment and respond appropriately to induce T-cell immunity, or possibly tolerance. In the lymph node, DC are also influenced by anti-

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“…By further extension, the MPS in general can be affected by a carcinoma. Such an occurrence is important to define because a generally changed MPS in cancer patients offers some explanation as to why certain patients will develop metastases, since monocytes and tissue macrophages have been shown to act as defense cells against tumor spread [26].…”

Section: Introductionmentioning

confidence: 99%

Ex vivo interleukin (IL)-1β, IL-6, IL-12 and tumor necrosis factor-α responsiveness with monocytes from patients with head and neck carcinoma

Heimdal

Aarstad

Klementsen

et al. 1999

European Archives of Oto-Rhino-Laryngology

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Seventy newly diagnosed Caucasian male patients with head and neck squamous cell carcinomas (HNSCC) were included in the study. All patients were less than 80 years of age, with no cachexia or auto-immune disease, and they were not taking immuno-active medications. Monocytes from these patients were cultured in vitro and supplemented with autologous serum under ex vivo conditions or cultured with serum-free medium. Comparison was made to monocytes from 59 patients with benign HN diseases. Similar physical activity levels prior to testing as well as a minimum stress load were ensured in both groups. Increased monocyte supernatant levels were determined under ex vivo conditions of interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and tumor necrosis factor (TNF)-alpha, but not of interleukin-12 (IL-12) with endotoxin stimulated monocytes of HNSCC patients compared to control conditions. Increased monokine levels were not present with mononuclear cell cultures stimulated with a T-cell general stimulatory agent or with purified monocytes not specifically stimulated. With endotoxin-stimulated monocytes under in vitro conditions, an increased supernatant was shown for TNF-alpha, but not IL-6. With serum from the different patients cultured with monocytes employed from a healthy control, no difference between the groups was shown in the IL-6 and TNF-alpha response to endotoxin stimulation. The differences in IL-1 beta and TNF-alpha, but not IL-6 levels were differentiated statistically from the smoking and alcohol histories of the patients. These findings suggest that the function of monocytes in general, and thus possibly all mononuclear phagocyte system cells in HNSCC patients, are altered.

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Tumour Immunology: Alternative Perspectives

Cited by 7 publications

References 47 publications

Vitronectin in human hepatic tumours contributes to the recruitment of lymphocytes in an αvβ3-independent manner

Vitronectin in human hepatic tumours contributes to the recruitment of lymphocytes in an αvβ3-independent manner

Dendritic cell activation by danger and antigen‐specific T‐cell signalling

Ex vivo interleukin (IL)-1β, IL-6, IL-12 and tumor necrosis factor-α responsiveness with monocytes from patients with head and neck carcinoma

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