Tumour necrosis factor alpha in gingival crevicular fluid as a possible indicator of periodontal disease in humans

Rossomando, Edward F.; Kennedy, James E.; Hadjimichael, Jane

doi:10.1016/0003-9969(90)90205-o

Cited by 212 publications

(144 citation statements)

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“…It has been shown by many investigators that IL-lo-, IL-11, IL-6, IL-8, and TNF-ot can be detected in GCF (Rossomando et al, 1990;Geivelis et al, 1993;Payne et al, 1993). Some of those reports suggested that the cytokine levels in GCF were closely associated with the severity of inflammatory responses and/or periodontal tissue destruction (Masada et al, 1990;Stashenko et al, 1991).…”

Section: Cytokine Expression and Periodontal Disease Statusmentioning

confidence: 99%

Cytokine Expression in Periodontal Health and Disease

Okada

Murakami

1998

Critical Reviews in Oral Biology & Medicine

505

504

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Section: Cytokine Expression and Periodontal Disease Statusmentioning

confidence: 99%

Cytokine Expression in Periodontal Health and Disease

Okada

Murakami

1998

Critical Reviews in Oral Biology & Medicine

505

504

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“…Cytokines are rapidly synthesized and increased levels of proinflammatory cytokines, such as tumor necrosis factor (TNF)-a, interleukin (IL)-1b, and IL-6, are detected in the inflamed gingival tissue and gingival crevicular fluid at inflamed site. [3][4][5] It is well demonstrated that these proinflammatory cytokines having bone resorbing activity, act osteoblasts (OBs) and stimulate osteoclastogenesis through upregulation of receptor activator of NFkB (RANKL) and downregulation of osteoprotegerin (OPG) in OBs. 6 Earlier, we have reported that initial periodontal tissue damage was provoked by topical application of Escherichia coli LPS into the rat gingival sulcus; infiltration of numerous polymorphonuclear leucocytes in the junctional epithelium (JE), 7 transient accumulation of exudative macrophages, 8 vascular dilatation and inflammatory edema in the sub-JE, enhancement of proliferative activity of JE cells, 9 and stimulation of osteoclastic bone resorption.…”

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confidence: 99%

Inhibitory effects of orally administrated liposomal bovine lactoferrin on the LPS-induced osteoclastogenesis

Yamano

Miyauchi

Furusyo

et al. 2010

Laboratory Investigation

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Bovine lactoferrin (bLF) modulates the production of proinflammatory cytokines including tumor necrosis factor (TNF)-a, and may thus control alveolar bone destruction associated with periodontitis. In this study, the effects of bLF on mRNA expression in lipopolysaccharide (LPS)-stimulated osteoblasts (OBs) and on LPS-induced osteoclastogenesis were examined. The inhibitory effects of oral administration of liposomal-bLF (L-bLF), which improved the robustness of bLF to digestive enzymes, on alveolar bone resorption using LPS-induced periodontitis rat model are also reported. Three groups of 7-week-old male Wistar rats were treated with L-bLF (L-bLF group), bLF (bLF group), or the vehicle (control group) in drinking water (n ¼ 6 in each group). On day 7, LPS was topically applied into the gingival sulcus. Number of osteoclasts and immunoexpression of TNF-a were analyzed. The bLF inhibited the upregulation of TNF-a-mRNA-and upregulation of receptor activator of NFkB (RANKL)-mRNA expression and eliminated downregulation of osteoprotegerin (OPG)-mRNA expression in LPS-stimulated OBs and reduced LPS-induced osteoclastogenesis in co-culture with primary OBs and bone marrow cells. In the control group, the number of osteoclasts increased after LPS treatment. The number of osteoclasts that appeared along the alveolar bone margin was significantly reduced (Po0.01) in the L-bLF but not in the bLF group. Furthermore, L-bLF suppressed upregulation of TNF-a immunoexpression in periodontal tissue and TNF-a and interleukin (IL)-1b-mRNA level in gingival tissue. The results of this study indicate that oral administration of L-bLF significantly reduces alveolar bone resorption induced by LPS stimulation through inhibition of TNF-a production and modulation of RANKL/OPG balance in OBs. It is suggested that L-bLF could be a potent therapeutic and preventive agent for attenuating alveolar bone destruction in periodontitis patients.

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“…Our study showed that the GCF TNF-α levels were significantly higher in periodontitis patients than healthy controls. In previous periodontal studies it was indicated that TNF-α can reach high concentration in the diseased gingival tissue and reported the higher levels of TNF-α in the GCF of periodontitis patients [4][5][6]25 . Our result confirms findings of these previous studies.…”

Section: Discussionmentioning

confidence: 97%

“…Increased levels of several cytokines such as Tumour necrosis factor-alpha (TNF-α) have been observed in the GCF of periodontitis patients. [4][5][6] TNF-α is a polypeptide produced by a wide variety of cell types whose production is up-regulated in a number of stressful and pathological conditions. 7,8 It has been shown that TNF-α exerts an important physiological role as a modulator of immune responses by regulating specific genes needed for the host defense against a varied repertoire of agents.…”

Section: Introductionmentioning

confidence: 99%

Tumour Necrosis Factor Alpha Levels in Gingival Crevicular Fluid of Periodontitis Patients With/Without Mtdna Deletion in Gingival Tissue

Çanakçı¹,

Doğan²

2015

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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Aim: There are strong evidences to suggest that Tumour necrosis factor alpha (TNF-α) plays important role in the pathogenesis of destructive periodontal pathologies such as periodontitis. Excessive amounts increase reactive oxygen species (ROS) production by mitochondria, which are likely to damage cellular macromolecules, including mtDNA. The 5-kbp mtDNA deletion of gingival tissue was detected in destructive periodontal pathologies. In this study we aimed to investigate the gingival crevicular fluid (GCF) levels of TNF-α and the 5-kbp mtDNA deletion of gingival tissue in patients with periodontitis and healthy controls. Also, we examined correlations between the GCF levels of TNF-α and the 5-kbp mtDNA deletion of gingival tissue and clinical parameters of periodontitis patients. Material and Method:The study was carried out in 64 subjects: 32 patients with chronic periodontitis (CP) and 32 periodontally healthy controls. Samples were collected from the diseased gingival tissues with ≥5mm periodontal pockets, gingival inflammation, and bone loss during periodontal flap surgery from CP group and healthy gingival tissues were collected during crown lengthening procedures. TNF-α levels in GCF samples were assayed by commercial ELISA kits. Determination of the deleted mtDNA was employed a semiquantitative PCR method. Results: All clinical parameter scores of the tissue sampling areas were significantly higher in the CP group compared to the control group. The mean GCF TNF-α level in CP group was significantly higher than that of the controls. In CP group, the mean GCF TNF-α level of patients with deleted mtDNA was significantly higher than those of patients with non-deleted mtDNA Conclusion: According to this study findings, we speculated that overexpression of TNF-α, which is detected in GCF, in gingival tissue of periodontitis patients, may induces elevated ROS production in mitochondria and may lead to oxidative mitochondrial injury such as mtDNA deletion of neighboring cells.

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Tumour necrosis factor alpha in gingival crevicular fluid as a possible indicator of periodontal disease in humans

Cited by 212 publications

References 11 publications

Cytokine Expression in Periodontal Health and Disease

Cytokine Expression in Periodontal Health and Disease

Inhibitory effects of orally administrated liposomal bovine lactoferrin on the LPS-induced osteoclastogenesis

Tumour Necrosis Factor Alpha Levels in Gingival Crevicular Fluid of Periodontitis Patients With/Without Mtdna Deletion in Gingival Tissue

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