Tumour response of osteosarcoma to neoadjuvant chemotherapy evaluated by magnetic resonance imaging as prognostic factor for outcome

Laux, Christoph J.; Berzaczy, Gundula; Weber, Michael; Lang, Susanna; Dominkus, Martin; Windhager, Reinhard; Nöbauer-Huhmann, I.-M.; Funovics, Philipp

doi:10.1007/s00264-014-2606-5

Cited by 25 publications

(12 citation statements)

References 24 publications

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“…Approximately 30% of OS patients are diagnosed with metastatic disease, especially pulmonary metastasis, and yet nearly two thirds of these patients are cured with surgical resection and systemic chemotherapy [Hou et al, 2014]. Although survival rates have improved significantly in OS patients due to effective chemotherapy and surgical intervention strategies, the overall survival has reached a plateau at 60-70% for the past 30 years [Xiao et al, 2014;Laux et al, 2015]. The propensity of OS cells to disseminate to the lung is the major cause of lung cancer, and lung metastasis is the main cause of death in OS patients [Munajat et al, 2008;Siegel et al, 2013;Kunz et al, 2015].…”

confidence: 99%

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

Xiao

Chen²,

Wang³

et al. 2015

Cytogenet Genome Res

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miR-150 expression in osteosarcoma (OS) cell lines and human osteoblast cells was detected, and OS cell models were transfected with exogenous miR-150 to investigate its role in cell proliferation, invasion, and apoptosis. Our results showed that miR-150 expression in OS cells (MG63, Saos-2, SOSP-9607, and U2OS) was significantly lower compared to the osteoblast hFOB1.19 cell line (all p < 0.01). The expression level of miR-150 in MG63 cells that were transfected with exogenous miR-150 mimics was markedly upregulated, while the miR-150 expression level in the inhibitor group was significantly downregulated (both p < 0.01). Similar results were also found in SOSP-9607 cells. Importantly, exogenous miR-150 expression stimulated cell apoptosis and inhibited proliferation, invasion, and migration. A luciferase reporter assay displayed that miR-150 also regulated Sp1 expression by targeting its 3′-UTR, and qRT-PCR and Western blotting showed that elevated levels of miR-150 may reduce Sp1 protein expression. The mRNA and protein levels of Sp1 were upregulated after transfection with a Sp1-expression plasmid and partially reversed the inhibitory effects of miR-150 on cell proliferation, invasion, and metastasis in MG63 and SOSP-9607 cells, as well as promoted cell apoptosis. In conclusion, miR-150 inhibits cell proliferation, invasion, and metastasis and stimulates cell apoptosis by regulating the expression of Sp1. Therefore, miR-150 may be a potential clinical target for the treatment of OS patients.

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confidence: 99%

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

Xiao

Chen²,

Wang³

et al. 2015

Cytogenet Genome Res

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“…Up to now, postoperative pathological tumour necrosis rate is the main criterion to evaluate the efficacy of NACT [4], but is invasive and cannot be used for real-time monitoring during NACT, or for guiding the choice of surgical timing and surgical plan, as it can be performed only postoperatively [5,6]. Moreover, the method requires pathologists to conduct extensive information processing to interpret highly complex pathological images, making it cumbersome, costly, time-consuming, and subjective [7].…”

Section: Introductionmentioning

confidence: 99%

Feasibility of multi-parametric magnetic resonance imaging combined with machine learning in the assessment of necrosis of osteosarcoma after neoadjuvant chemotherapy: a preliminary study

et al. 2020

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Background: Response evaluation of neoadjuvant chemotherapy (NACT) in patients with osteosarcoma is significant for the termination of ineffective treatment, the development of postoperative chemotherapy regimens, and the prediction of prognosis. However, histological response and tumour necrosis rate can currently be evaluated only in resected specimens after NACT. A preoperatively accurate, noninvasive, and reproducible method of response assessment to NACT is required. In this study, the value of multi-parametric magnetic resonance imaging (MRI) combined with machine learning for assessment of tumour necrosis after NACT for osteosarcoma was investigated. Methods: Twelve patients with primary osteosarcoma of limbs underwent NACT and received MRI examination before surgery. Postoperative tumour specimens were made corresponding to the transverse image of MRI. One hundred and two tissue samples were obtained and pathologically divided into tumour survival areas (non-cartilaginous and cartilaginous tumour viable areas) and tumour-nonviable areas (non-cartilaginous tumour necrosis areas, post-necrotic tumour collagen areas, and tumour necrotic cystic/haemorrhagic and secondary aneurismal bone cyst areas). The MRI parameters, including standardised apparent diffusion coefficient (ADC) values, signal intensity values of T2-weighted imaging (T2WI) and subtract-enhanced T1-weighted imaging (ST1WI) were used to train machine learning models based on the random forest algorithm. Three classification tasks of distinguishing tumour survival, non-cartilaginous tumour survival, and cartilaginous tumour survival from tumour nonviable were evaluated by five-fold cross-validation.

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“…30 After chemotherapy, volume reduction in the extraosseous part and progressive sclerosis of an OS, leading to lower T2w signal, is indicative of response. 31 Metastasis should be considered in the differential diagnosis of a solitary or multiple sclerotic lesions in any patient older than 40 years. Sclerotic metastases are predominantly lowT2wSI and lowT1wSI (►Fig.…”

Section: Focal T2 Hypointense Bone Tumors and Tumor-like Lesionsmentioning

confidence: 99%

T2-weighted Hypointense Tumors and Tumor-like Lesions

Papakonstantinou

Isaac

Dalili

et al. 2019

Semin Musculoskelet Radiol

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Most musculoskeletal tumors are hyperintense on T2-weighted images. However, some T2-weighted hypointense tumors and tumor-like lesions are encountered in everyday clinical practice. We explore the spectrum of such T2 hypointense tumors and tumor mimickers that can arise from (1) the bones, presenting as diffuse processes or focal lesions; (2) the joints including diffuse or focal synovial disorders, loose bodies, or substance depositions; and (3) soft tissues, comprising T2 hypointense tumors and tumor mimickers (those that contain abundant fibrous tissue, mineralization, or hemosiderin deposits). Appropriate magnetic resonance imaging sequence selection is required to identify and characterize these lesions confidently when imaging musculoskeletal tumors.

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Tumour response of osteosarcoma to neoadjuvant chemotherapy evaluated by magnetic resonance imaging as prognostic factor for outcome

Abstract: Separated volumetry of tumour segments revealed interesting insights into therapy-induced growth patterns. If verified in a larger study population, these results should be taken into account when planning ablative surgery.

Cited by 25 publications

References 24 publications

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

Feasibility of multi-parametric magnetic resonance imaging combined with machine learning in the assessment of necrosis of osteosarcoma after neoadjuvant chemotherapy: a preliminary study

T2-weighted Hypointense Tumors and Tumor-like Lesions

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