Tumourigenic phenotypes of human melanoma cell lines in nude mice determined by an active antitumour mechanism

Abstract

Summary Ten human melanoma cell lines (HMCL) were tested for their ability to grow subcutaneously in nude mice. Using a standard inoculum, the HMCL could be characterized by their highly, fairly or poorly xenografting phenotype. These phenotypes were stable and the phenotype of one HMCL was recovered within cell clones derived from it. The role of nude mice natural defences in the expression of HMCL xenografting phenotypes was studied. Sublethal whole body irradiation and silica pretreatment of recipients enabled poorly tumourigenic HMCL to grow in most animals without affecting their splenic NK activity. Admixture of BCG or MDP encapsulated in liposomes with highly tumourigenic HMCL resulted in the abrogation of tumour growth in naive nude mice. The long lasting abrogating of NK activity in vivo by treatment with anti-asialo-GM1 anti-serum did not enhance the growth of a poorly tumourigenic HMCL. The HMCL were found to be resistant to in vitro murine NK activity. These results showed that the expression of the HMCL xenografting phenotypes could be controlled by the nude mice natural defences. NK cells did not seem to be largely involved whereas macrophages might be good candidates as anti-xenograft effectors.

UVB-Induced G2 Arrest of Human Melanocytes Involves Cdc2 Sequestration by Gadd45a in Nuclear Speckles

“…The human melanoma cell line M4Be, previously established in the laboratory, 21 was cultured in MacCoy's 5A medium (Cambrex), supplemented with 10% foetal calf serum, 2 mM glutamine, 200 IU/ml penicillin and 20 µg/ml streptomycin. Primary antibodies to Gadd45a (sc-797), PML (sc-966), Gadd45a, p21 Waf1 and Cdc2 Localization in Nuclear Speckles p21 Waf1 (sc-397), Cdc2 (sc-54) and β-actin (sc-1616) were commercially available from Santa Cruz Biotechnology (Tebu-bio, France).…”

Specific induction of gadd45 in human melanocytes and melanoma cells after UVB irradiation

“…M4Be human melanoma cells were previously established in the laboratory. 9 WM983 cells were obtained from Dr. M. Herlyn. 10 Cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FCS, 200 U/ml penicillin and 20 g/ml streptomycin at 37°C in a humidified 5% CO 2 incubator.…”

Plasminogen activation by t-PA on the surface of human melanoma cells in the presence of alpha 2-macroglobulin secretion.

“…Svec, Cancer Research Institute, Bratislava, Czechoslovakia (Siracjky et al, 1982;Svec et al, 1988). The parental HMB-2 cell line and clones 15 and 33, also used in this study, have been described in detail (Bizik etal., 1989) by Dr. J. F. Dor6, Centre Leon B6rard, Lyon, France (Jakubovich et al, 1985). The human RD rhabdomyosarcoma cell line (CCL 136) was obtained from the American type Culture Collection, Rockville, MD.…”