Tuna Oil Alleviates d-Galactose Induced Aging in Mice Accompanied by Modulating Gut Microbiota and Brain Protein Expression

Abstract

To discern whether tuna oil modulates the expression of brain proteins and the gut microbiota structure during aging induced by d-galactose, we generated an aging mouse model with d-galactose treatment, and the mice showed aging and memory deterioration symptoms according to physiological and biochemical indices. Treatment with different doses of tuna oil alleviated the symptoms; the high dose showed a better effect. Subsequently, brain proteomic analysis showed the differentially expressed proteins were involved in damaged synaptic system repairment and signal transduction system enhancement. In addition, tuna oil treatment restored the diversity of gut microbiota, 27 key operational taxonomic units, which were identified using a redundancy analysis and were significantly correlated with at least one physiological index and three proteins or genes. These findings suggest that the combination of proteomics and gut microbiota is an effective strategy to gain novel insights regarding the effect of tuna oil treatment on the microbiota-gut-brain axis.

Roles of Gut Microbiota in the Regulation of Hippocampal Plasticity, Inflammation, and Hippocampus-Dependent Behaviors

“…GF, Germ free; LJ, Lactobacillus johnsonii CJLJ103; VSL3#, a probiotic mixture comprising 8 Gram-positive bacterial strains; caf, cafeteria; LTP, long-term potentiation, the cellular mechanism of synaptic plasticity; TTK, Tetragonia tetragonioides Kuntze extract; R2*, a validated magnetic resonance imaging (MRI) marker of brain iron content which can be rapidly measured under clinical conditions. The taxonomic group of bacteria: Phylum, Class, Order, Family, Genus, Species, were marked with p, c, o, f, g, s. and changed the expression of proteins related to synaptic repair and signal transduction (Zhang et al, 2018). Additionally, treatment of LW-AFC, an herbal medicine prepared from the traditional Chinese medicine LiuweiDihuang decoction, was given to senescence-accelerated mouse prone 8 (SAMP8) mice, which resulted in improvement of cognitive impairments including spatial learning and memory, active avoidance response, and object recognition memory capability.…”

Docosahexaenoate-enriched fish oil and medium chain triglycerides shape the feline plasma lipidome and synergistically decrease circulating gut microbiome-derived putrefactive postbiotics

“…LCPUFA(n3) such as eicosapentaenoate (EPA; C20:5n3) and docosahexaenoate (DHA; C22:6n3) are notable for their impact on inflammatory processes, and intake of these oils has recently been shown to influence composition of the gut microbiome [8,20]. Consumption of fish and krill seafood oils rich in polyunsaturated fatty acids has been shown to impact microbiome community structure as well as the abundances of particular microbes that are associated with physiologic traits such as obesity [8,21] and brain aging [22]. Whereas LCPUFA(n3) are associated with increased beneficial gut microbiota, LCPUFA (n6) have been shown to have detrimental effects on gut barrier function and microbiome composition [7,23].…”

Protective Effects of Water Extract of Fructus Ligustri Lucidi against Oxidative Stress-Related Osteoporosis In Vivo and In Vitro

“…Accordingly, the galactitol, which cannot be adequately metabolized, accumulated in the body and caused cell swelling and death [ 22 ]. Simultaneously, large amounts of free radicals produced during metabolism contributed to cellular damage and dysfunction, ultimately accelerating oxidative stress and aging in the body [ 23 ]. After modeling, the malondialdehyde (MDA) contents in the liver and kidney of mice increased and superoxide dismutase (SOD) activity decreased, which were evident manifestations of oxidative damage ( p < 0.01, Figure 3 ).…”