Tunable Assembly of Peptide-coated Gold Nanoparticles

Tullman, Jennifer; Finney, William F.; Lin, Yu‐Jen; Bishnoi, Sandra Whaley

doi:10.1007/s11468-007-9033-z

Cited by 25 publications

(22 citation statements)

References 26 publications

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“…35 The spectra displayed minute amounts of noise at higher wavelengths, which is expected for silica gold nanoshells. 36 Nanoshells demonstrated ideal optical properties for near-infrared (NIR) application, 37,38 though such experiments were not included in the scope of this work.…”

Section: Nanoparticle Characterizationmentioning

confidence: 99%

Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles

England¹,

Priest²,

Zhang³

et al. 2013

IJN

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Nano-scale particles sized 10–400 nm administered systemically preferentially extravasate from tumor vasculature due to the enhanced permeability and retention effect. Therapeutic success remains elusive, however, because of inhomogeneous particle distribution within tumor tissue. Insufficient tumor vascularization limits particle transport and also results in avascular hypoxic regions with non-proliferating cells, which can regenerate tissue after nanoparticle-delivered cytotoxicity or thermal ablation. Nanoparticle surface modifications provide for increasing tumor targeting and uptake while decreasing immunogenicity and toxicity. Herein, we created novel two layer gold-nanoshell particles coated with alkanethiol and phosphatidylcholine, and three layer nanoshells additionally coated with high-density-lipoprotein. We hypothesize that these particles have enhanced penetration into 3-dimensional cell cultures modeling avascular tissue when compared to standard poly(ethylene glycol) (PEG)-coated nanoshells. Particle uptake and distribution in liver, lung, and pancreatic tumor cell cultures were evaluated using silver-enhancement staining and hyperspectral imaging with dark field microscopy. Two layer nanoshells exhibited significantly higher uptake compared to PEGylated nanoshells. This multilayer formulation may help overcome transport barriers presented by tumor vasculature, and could be further investigated in vivo as a platform for targeted cancer therapies.

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Section: Nanoparticle Characterizationmentioning

confidence: 99%

Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles

England¹,

Priest²,

Zhang³

et al. 2013

IJN

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“…The ones selected via cell surface display include gold (Brown 1997) and zinc oxide (Kjaergaard et al 2000), whereas phage display selected ones are for their affinity towards gallium arsenide (Whaley et al 2000), silica (Naik et al 2002a;Eteshola et al 2005), silver (Naik et al 2002b), zinc sulphide (Lee et al 2002a), calcite (Li et al 2002), cadmium sulphide (Mao et al 2003) and titanium oxide (Sano et al 2005). Some biocombinatorially selected peptides have been used to assemble inorganic particles (Whaley et al 2000;Lee et al 2002a,b;Mao et al 2003;Matthes et al 2008) or to control nucleation of the compounds that they were selected for (Li et al 2002;Naik et al 2002a,b;Tullman et al 2008).…”

Section: Genetic Selection and Directed Evolution Of Solid-binding Pementioning

confidence: 99%

Molecular biomimetics: nanotechnology and bionanotechnology using genetically engineered peptides

Tamerler

Sarıkaya

2009

Phil. Trans. R. Soc. A.

101

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Nature provides inspiration for designing materials and systems that derive their functions from highly organized structures. Biological hard tissues are hybrid materials having inorganics within a complex organic matrix, the molecular scaffold controlling the inorganic structures. Biocomposites incorporate both biomacromolecules such as proteins, lipids and polysaccharides, and inorganic materials, such as hydroxyapatite, silica, magnetite and calcite. The ordered organization of hierarchical structures in organisms begins via the molecular recognition of inorganics by proteins that control interactions and is followed by the highly efficient self-assembly across scales. Following the molecular biological principle, proteins could also be used in controlling materials formation in practical engineering via self-assembled, hybrid, functional materials structures. In molecular biomimetics, material-specific peptides could be the key in the molecular engineering of biology-inspired materials. With the recent developments of nanoscale engineering in physical sciences and the advances in molecular biology, we now combine genetic tools with synthetic nanoscale constructs to create a novel methodology. We first genetically select and/or design peptides with specific binding to functional solids, tailor their binding and assembly characteristics, develop bifunctional peptide/protein genetic constructs with both material binding and biological activity, and use these as molecular synthesizers, erectors and assemblers. Here, we give an overview of solid-binding peptides as novel molecular agents coupling bioand nanotechnology.

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“…[6][7][8] Length and charge of the peptide were considered based on the negatively charged surface of the citrate-capped AuNPs. 7,33 It is well known that conjugating with adequate ligands can improve the stability of AuNPs, and the stabilizing effect can be increased with the increase in length of the peptide. However, longer peptides may be broken easily, causing increased background for detection.…”

Section: Discussionmentioning

confidence: 99%

“…Five negatively charged amino acid molecules, namely aspartic acid (D) molecules, conjugated onto the AuNPs colloid, could increase the stability of the peptide-capped AuNPs and avoid AuNPs aggregation by increasing electrostatic repulsion between the peptides. 13,33 Moreover, negative charge on the spacer peptide could allow a well packed layer of peptides on the AuNP surface to increase enzyme access to the cleavage site.…”

Section: Discussionmentioning

confidence: 99%

Detection of chymase activity using a specific peptide probe conjugated onto gold nanoparticles

et al. 2018

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Gold nanoparticles (AuNPs) can be applied in biosensors using fluorescence resonance energy transfer (FRET) technique. Based on this technique, we have established a sensitive and efficient biosensing method by modifying a peptide-probe onto AuNPs to detect proteinase enzyme activity in this study.This biosensing method was designed for chymase activity detection and applied in kidney disease diagnosis. In this study, 16 nm-AuNPs were used to construct the AuNPs-based fluorescence peptide probe (named AuNPs-peptide probe) for chymase activity determination. The peptide sequence is FITC-Acp-DRVYIHPFHLDDDDDC, which comprises a fluorophore at the N-terminal end, an enzyme (chymase) substrate (DRVYIHPFHL), a spacer (DDDDD) and cysteine (C) to conjugate to AuNPs surface. When the enzyme catalyzes the substrate sequence, the fluorophore drifts away from AuNPs and the fluorescence emitting signal can be excited at 495 nm and detected at 515 nm. The results indicate that the time required for the AuNPs-peptide probe for activity detection of chymase was only 15 min, and a linear correlation from 10 to 100 ng mL À1 of chymase was acquired. The chymase reaction would be significantly inhibited by addition of specific chymase inhibitor chymostatin. The AuNPs-peptide probe was tested for the detection of high concentrations of trypsin and chymotrypsin, but only minor emitted fluorescence intensity was detected. According to these results, sensitivity and specificity of the AuNPspeptide probe for chymase detection have been confirmed. AuNPs-peptide probe was successfully used for the detection of renal chymase activity; and the results indicate the pathogenically increased chymase activity in kidney tissue of nephropathic mice from aristolochic acid I treatment.

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Tunable Assembly of Peptide-coated Gold Nanoparticles

Cited by 25 publications

References 26 publications

Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles

Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles

Molecular biomimetics: nanotechnology and bionanotechnology using genetically engineered peptides

Detection of chymase activity using a specific peptide probe conjugated onto gold nanoparticles

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