Tunable femtosecond laser suppresses the proliferation of breast cancer in vitro

Taha, Safaa; Mohamed, Wafaa R.; Elhemely, Mai A.; El-Gendy, Ahmed O.; Mohamed, Tarek A.

doi:10.1016/j.jphotobiol.2023.112665

Cited by 12 publications

(11 citation statements)

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“…Other types of laser beams should be considered for pest management, such as pulsed laser systems. In the interaction of a laser beam with a biological tissue, which in our context is a physical system plant-insect, the main advantage of using a pulsed laser system, with the same wavelength 454 nm, would be related to the reduction of thermal effects on the plant [32,33], which represents an unquestionable advantage. However, in the context of this work, which is to present a photonic technique that small farmers could use, the considerable drawback of a pulsed laser system is the cost factor compared to a CW DL system.…”

Section: Discussionmentioning

confidence: 99%

New Biocompatible Technique Based on the Use of a Laser to Control the Whitefly Bemisia tabaci

et al. 2023

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The whitefly Bemisia tabaci is among the most important agricultural pests in the world and one of the world’s top 10 most invasive insect pests. Bemisia tabaci is associated with severe yield and quality losses, mainly due to the transmission of plant viruses, as in the case of common beans (Phaseolus vulgaris L.). Reducing insecticide applications is a research priority, e.g., developing innovative and clean tools such as electromagnetic waves. The present work aims to determine the effective parameters of laser to reduce the Bemisia tabaci population in common beans. Preliminary assays were conducted by manually irradiating continuous-wave laser beams with different wavelengths (444 nm, 527 nm, and 640 nm) and optical intensities directly on the insects. Among these, the most effective wavelength was 444 nm. Later, we repeated the experiments using a homemade automated system to control the exposure time (t1 = 1 s, t2 = 2 s, t3 = 3 s and t4 = 4 s) of whiteflies to the incident beam at different optical intensities (I1 ≈ 10 Wcm−2, I2 ≈ 4 Wcm−2, I3 ≈ 2 Wcm−2). We have achieved 100% insect mortality by irradiating 454 nm laser wavelength on the 3rd instar nymphs of Bemisia tabaci, with the following parameters: I1(t1), I2(t3) and I3(t4). Moreover, the laser irradiation test did not affect plant yield and development, revealing that our preliminary results present a photonic technique that could control whiteflies without harming the plants’ development.

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Section: Discussionmentioning

confidence: 99%

New Biocompatible Technique Based on the Use of a Laser to Control the Whitefly Bemisia tabaci

et al. 2023

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“…To assess mitochondrial activity of hGFs, we used an assay based on the ability of mitochondrial dehydrogenases to reduce WST-8 to formazan. Compared to other tests using different tetrazolium salts (MTT, XTT, MTS or WST-1), the detection sensitivity using CCK-8 is higher and toxicity is lower [ 23 , 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Three Laser Wavelengths with Different Power Densities on the Mitochondrial Activity of Human Gingival Fibroblasts in Cell Culture

Nowak-Terpiłowska

Zeyland

Hryhorowicz

et al. 2023

Life

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Phototherapy plays a key role in wound healing and tissue regeneration. The use of lasers has the potential to become an effective and minimally invasive treatment in periodontal and peri-implant disease. The aim of this study was to evaluate the influence of three laser wavelengths with the combination of parameters such as power density and energy density on human gingival fibroblasts (hGFs) in vitro culture. Isolated cells were seeded in 96-well plates with culture medium (DMEM, Dulbecco’s modified Eagle’s medium) supplemented with 10% fetal bovine serum (FBS). After 24 h cells were irradiated (1064, 980 and 635 nm, various energy density value). After 24, 48 and 72 h, cells were evaluated for viability. Data were analyzed by ANOVA followed by Tukey’s HSD test. We found the best outcomes for hGFs irradiated with laser 1064 nm for all combinations of power output (50/400/1000 mW) and energy dose (3/25/64 J/cm2) after 48 h and 72 h compared with control group. Cell viability increase ranged from 0.6× (3 J/cm2, 50 mW) to 1.3× (64 J/cm2, 1000 mW). Our findings indicate that the appropriate use of low-level laser irradiation (LLLI) can increase the proliferation rate of cultured cells. The use of LLLI can be extremely useful in tissue engineering and regenerative medicine.

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“…Cell viability was not significantly influenced by wavelengths of 700, 720, 750, and 780 nm. Overall, femtosecond laser irradiation exhibited a noteworthy anticancer effect against T47D cells, suggesting its potential use in breast cancer management [ 78 ].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Resonance Imaging in Breast Cancer Tissue In Vitro after PDT Therapy

Bartusik-Aebisher,

Mytych,

Dynarowicz

et al. 2024

Diagnostics

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Photodynamic therapy (PDT) is increasingly used in modern medicine. It has found application in the treatment of breast cancer. The most common cancer among women is breast cancer. We collected cancer cells from the breast from the material received after surgery. We focused on tumors that were larger than 10 mm in size. Breast cancer tissues for this quantitative non-contrast magnetic resonance imaging (MRI) study could be seen macroscopically. The current study aimed to present findings on quantitative non-contrast MRI of breast cancer cells post-PDT through the evaluation of relaxation times. The aim of this work was to use and optimize a 1.5 T MRI system. MRI tests were performed using a clinical scanner, namely the OPTIMA MR360 manufactured by General Electric HealthCare. The work included analysis of T1 and T2 relaxation times. This analysis was performed using the MATLAB package (produced by MathWorks). The created application is based on medical MRI images saved in the DICOM3.0 standard. T1 and T2 measurements were subjected to the Shapiro–Wilk test, which showed that both samples belonged to a normal distribution, so a parametric t-test for dependent samples was used to test for between-sample variability. The study included 30 sections tested in 2 stages, with consistent technical parameters. For T1 measurements, 12 scans were performed with varying repetition times (TR) and a constant echo time (TE) of 3 ms. For T2 measurements, 12 scans were performed with a fixed repetition time of 10,000 ms and varying echo times. After treating samples with PpIX disodium salt and bubbling with pure oxygen, PDT irradiation was applied. The cell relaxation time after therapy was significantly shorter than the cell relaxation time before PDT. The cells were exposed to PpIX disodium salt as the administered pharmacological substance. The study showed that the therapy significantly affected tumor cells, which was confirmed by a significant reduction in tumor cell relaxation time on the MRI results.

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Tunable femtosecond laser suppresses the proliferation of breast cancer in vitro

Cited by 12 publications

References 0 publications

New Biocompatible Technique Based on the Use of a Laser to Control the Whitefly Bemisia tabaci

New Biocompatible Technique Based on the Use of a Laser to Control the Whitefly Bemisia tabaci

Influence of Three Laser Wavelengths with Different Power Densities on the Mitochondrial Activity of Human Gingival Fibroblasts in Cell Culture

Magnetic Resonance Imaging in Breast Cancer Tissue In Vitro after PDT Therapy

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