Tunable Self-Assembly of Genetically Engineered Silk–Elastin-like Protein Polymers

Xia, Xiao-Xia; Xu, Qiaobing; Hu, Xiao; Qin, Guokui; Kaplan, David L.

doi:10.1021/bm201165h

Cited by 205 publications

(272 citation statements)

References 34 publications

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“…Other SELRs have been reported to form nano-objects with different shapes, ranging from nanoparticles to nanofibers, depending on the conditions and the SL to EL ratio 28,57,58 . Along with the reported evidence for other SELRs and their ability to spontaneously form fibers, and considering that the only difference between (EI)x2 and (EIS)x2 is the presence of SL blocks in the latter, it can be concluded that these blocks are responsible for the ability of these materials to eventually organize into fibrils under the conditions studied.…”

Section: Microscopic Observationmentioning

confidence: 99%

“…However, the stimuli-responsiveness of the EL block has not been exploited to any great extent to date and no complex molecular designs for that block showing advanced functionality have been reported. Furthermore, many SELR designs incorporate such a high SL percentage that the contribution of the elastomeric portion to the self-assembly process is imperceptible 27,28 . However, experience with SELR designs with a lower SL content has demonstrated that, in such compositions, the EL blocks are able to maintain their characteristic thermal transition [28][29][30] .…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, many SELR designs incorporate such a high SL percentage that the contribution of the elastomeric portion to the self-assembly process is imperceptible 27,28 . However, experience with SELR designs with a lower SL content has demonstrated that, in such compositions, the EL blocks are able to maintain their characteristic thermal transition [28][29][30] . One of the main drawbacks associated with the use of SL motifs as physical crosslinking domains to obtain injectable hydrogels is that the kinetics of β-sheet formation under physiological conditions (aqueous medium, neutral pH and 37°C) is too slow 31 .…”

Section: Introductionmentioning

confidence: 99%

“…This is clearly different from the kinetics of ELR self-assembly, which is practically instantaneous above Tt. An interesting example of SELRs explored for their capacity to show dual elastin and silk associations have been reported 28 . Nevertheless, in that work the ratio SL to EL ranged between 15% to 60% so the portion of SL blocks was high and clearly dominated the selfassembling properties of the molecule giving no much space for exploiting the self-assembling peculiarities of the EL blocks.…”

Section: Introductionmentioning

confidence: 99%

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Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

et al. 2014

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ABSTRACT. Although significant progress has been made in the area of injectable hydrogels for biomedical applications and model cell niches, further improvements are still needed, especially in terms of mechanical performance, stability and biomimicry of the native fibrillar architecture found in the extracellular matrix (ECM). This work focuses on the design and production of a silk-elastin-based injectable multiblock co-recombinamer that spontaneously forms a stable physical nanofibrillar hydrogel under physiological conditions. That differs from previously reported silk-elastin-like polymers on a major content and predominance of the elastin-like part, as well as a more complex structure and behavior of such part of the molecule, which is aimed to obtain well defined hydrogels.Rheological and DSC experiments showed that this system displays a coordinated and concomitant dual gelation mechanism. In a first stage, a rapid, thermally driven gelation of the co-recombinamer solution takes place once the system reaches body temperature due to the thermal responsiveness of the elastinlike (EL) parts and the amphiphilic multiblock design of the co-recombinamer. A bridged micellar 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 structure is the dominant microscopic feature of this stage, as demonstrated by AFM and TEM.Completion of the initial stage triggers the second, which comprises a stabilization, reinforcement, and microstructuring of the gel. FTIR analysis shows that these events involve the formation of β-sheets around the silk motifs. The emergence of such β-sheet structures leads to the spontaneous selforganization of the gel into the final fibrous structure. Despite the absence of biological cues, here we set the basis of the minimal structure that is able to display such a set of physical properties and undergo microscopic transformation from a solution to a fibrous hydrogel. The results point to the potential of this system as a basis for the development of injectable fibrillar biomaterial platforms towards a fully functional, biomimetic, artificial extracellular matrix and cell niches.

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Section: Microscopic Observationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

et al. 2014

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“…Such properties are maintained when GAGAGS motifs are engineering along an ELR molecule, giving rise to so-called SELR (silk-elastin like recombinamer). Many SELR-based hydrogels have been reported in the literature [94][95][96][97] and some of them have been successfully explored for drug-delivery purposes. [98][99][100][101] Indeed, the utility of SELRs has been explored in both the field of drug delivery and for the delivery of viral vectors.…”

mentioning

confidence: 99%

Nanotechnological Approaches to Therapeutic Delivery Using Elastin-Like Recombinamers

et al. 2015

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This review discusses the use of elastin-like polymers and their recombinant version, elastinlike recombinamers, in drug-delivery systems. These macromolecules exhibit a number of interesting properties that are rarely found together in any other family of materials, especially extremely high biocompatibility, high bioactivity and functionality, complex yet fully controlled composition and stimuli responsiveness.Appropriate design of these molecules opens up a broad range of different possibilities for their use in new therapeutic platforms. The first of these described herein is the use of ELRs in singlemolecule devices as therapeutic entities on their own. Subsequently, we describe how the selfassembly properties of these materials can be exploited to create nanocarriers and, eventually, microcarriers that are able to temporally and spatially control and direct the release of their drug load. Intracellular drug-delivery devices and nanocarriers for treating cancer are among the uses described in that section. Finally, the use of ELRs as base materials for implantable drug depots, in the form of hydrogels, is discussed.

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Future Trends for Recombinant Protein‐Based Polymers: The Case Study of Development and Application of Silk‐Elastin‐Like Polymers

2013

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Tunable Self-Assembly of Genetically Engineered Silk–Elastin-like Protein Polymers

Cited by 205 publications

References 34 publications

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

Nanotechnological Approaches to Therapeutic Delivery Using Elastin-Like Recombinamers

Future Trends for Recombinant Protein‐Based Polymers: The Case Study of Development and Application of Silk‐Elastin‐Like Polymers

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