Tunable Synthesis of Encapsulated Microbubbles by Coupled Electrophoretic Stabilization and Electrochemical Inflation

Abstract

Mit bloßem Auge sichtbar: Ein Wechselspiel zwischen Elektrophorese und Elektrochemie kann genutzt werden, um makromolekulare Spezies wie DNA, Proteine oder Cyclodextrane an einer Elektrodenoberfläche zu kompaktieren und gleichzeitig den Molekülfilm mittels Gasblasen aus einer lokalen elektrochemischen Reaktion aufzublähen. Der kompaktierte Film ist selbst bei unmarkierten Proben unter gewöhnlichem weißem Licht sichtbar, woraus sich eine markierungsfreie Technik für den Nachweis von Makromolekülen wie DNA, Proteinen und Cyclodextranen ergibt.

Smartphone-based detection of unlabeled DNA via electrochemical dissolution

“…Design, construction, and operation of the microfabricated electrode array devices has been described in detail previously. 19,20 Briey, silicon wafers (P(100), 500 mm thick, 15 cm diameter, 5000 Å oxide layer; University Wafer), were cleaned in a reactive ion etcher, spin coated with hexamethyldisilazane (J.T. Baker) followed by a positive photoresist (Shipley 1827; Rohm & Haas), patterned, and developed (MF-319 developer; Rohm & Haas).…”

“…1)an arrangement that allows very small potentials (2.5 V) to generate locally high electric elds (hundreds of V cm À1 ). 19,20 Electrode dissolution is monitored by recording the change in surface reectivity with time under oblique illumination with ordinary white light, from which the average intensity within a region of interest centered on the electrode can be quantied. In this way, changes in dissolution rate in response to the bulk solution's chemical composition can be readily distinguished.…”