Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide)

Satti, Angel José; Espeel, Pieter; Martens, Steven; Hoeylandt, Tim Van; Prez, Filip Du; Lynen, Frédéric

doi:10.1016/j.chroma.2015.11.063

Cited by 14 publications

(14 citation statements)

References 34 publications

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“…Alongside columns based on homo-PNIPAAm, several co-polymers including NIPAAm have been tested as well, as they lead to a modification of the LCST and induce slightly different retention properties. [35][36][37][38][39][40][41][42] Other polymers that have successfully been used in TR columns include polyoxazoline (LCST controllable) and polyvinylcaprolactam (LCST 35ºC). 43,44 The possibility of applying TRLC in heart-cutting 2D-LC (LC-LC) has recently been illustrated by Kanazawa et al, where a TR column was used as a pretreatment column.…”

Section: Temperature-responsive Chromatographymentioning

confidence: 99%

“…Furthermore, the typical cloud point of the polymer (32 °C) is situated in a convenient temperature range where the viscosity of water is not excessive, the hydrothermal stability of the (silica) supporting materials is not exceeded, and where analyte degradation is also improbable. Alongside columns based on homo-PNIPAAm, several copolymers including NIPAAm have been tested as well, as they lead to a modification of the LCST and induce slightly different retention properties. − Other polymers that have successfully been used in TR columns include polyoxazoline (LCST controllable) and polyvinylcaprolactam (LCST 35 °C). , …”

Section: Introductionmentioning

confidence: 99%

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Enhancing the Possibilities of Comprehensive Two-Dimensional Liquid Chromatography through Hyphenation of Purely Aqueous Temperature-Responsive and Reversed-Phase Liquid Chromatography

Baert¹,

Martens²,

Desmet

et al. 2018

Anal. Chem.

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Comprehensive two-dimensional liquid chromatography (LC × LC) allows for substantial gains in theoretical peak capacity in the field of liquid chromatography. However, in practice, theoretical performance is rarely achieved due to a combination of undersampling, orthogonality, and refocusing issues prevalent in many LC × LC applications. This is intricately linked to the column dimensions, flow rates, and mobile-phase compositions used, where, in many cases, incompatible or strong solvents are introduced in the second-dimension (D) column, leading to peak broadening and the need for more complex interfacing approaches. In this contribution, the combination of temperature-responsive (TR) and reversed-phase (RP) LC is demonstrated, which, due to the purely aqueous mobile phase used in TRLC, allows for complete and more generic refocusing of organic solutes prior to the second-dimension RP separation using a conventional 10-port valve interface. Thus far, this was only possible when combining other purely aqueous modes such as ion exchange or gel filtration chromatography with RPLC, techniques which are limited to the analysis of charged or high MW solutes, respectively. This novel TRLC × RPLC combination relaxes undersampling constraints and complete refocusing and therefore offers novel possibilities in the field of LC × LC including temperature modulation. The concept is illustrated through the TRLC × RPLC analysis of mixtures of neutral organic solutes.

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Section: Temperature-responsive Chromatographymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancing the Possibilities of Comprehensive Two-Dimensional Liquid Chromatography through Hyphenation of Purely Aqueous Temperature-Responsive and Reversed-Phase Liquid Chromatography

Baert¹,

Martens²,

Desmet

et al. 2018

Anal. Chem.

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“…A. J. Satti et al reported that the incorporation of selected moieties next to the temperature-responsive polymers has a strong influence on the retention behavior 52 .…”

Section: Temperature-responsive Chromatography Utilizing a Functional Polymer Surfacementioning

confidence: 99%

Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography

et al. 2021

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In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, and regenerative medicine. Therefore, new approaches and technologies must be developed to respond to these changes in medical care. To achieve this, we applied a temperature-responsive separation system to purify a variety of proteins and cells. We developed a temperatureresponsive chromatography technique based on a poly(N-isopropylacrylamide) (PNIPAAm)-grafted stationary phase. This method may be applied to various types of protein and cell separation applications by optimizing the properties of the modified polymers used in this system. Therefore, the developed temperature-responsive HPLC columns and temperature-responsive solid phase extraction (TR-SPE) columns can be an effective separation tool for new therapeutic modalities such as monoclonal antibodies, nucleic acid drugs, and cells.

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“…Poly(thiolactone acrylamide) (PTlaAm) is of particular interest in this work due to its ability for double modification through thiolactone rings. The rings can readily react with nucleophiles and essentially form thiol (SH) groups, which can subsequently react with double or triple bond, epoxide, and alkyl halide and also formation of disulfide linkages . In the first step, PPEGMA prepared through reversible addition‐fragmentation chain transfer (RAFT) polymerization was used as a macro chain transfer agent (CTA) for an extension of PNIPAAm‐ st ‐PTlaAm second block.…”

Section: Introductionmentioning

confidence: 99%

Water dispersible magnetite nanocluster coated with thermo‐responsive thiolactone‐containing copolymer

Paenkaew

Kajornprai

Rutnakornpituk

2020

Polymers for Advanced Techs

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This work focused on surface modification of magnetite nanoparticle (MNP) with poly(poly(ethylene glycol) monomethyl ether methacylate)-b-(poly(N-isopropylacrylamide)-st-poly(thiolactone acrylamide)), PPEGMA-b-(PNIPAAm-st-PTlaAm), diblock copolymer, synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization to obtain the particles having good water dispersible PPEGMA brushes, thermo-responsive PNIPAAm, and reactive thiolactone groups of PTlaAm.The thiolactone moiety in the copolymer can readily react with amino groups grafted on MNP surface and essentially induced the formation of MNP nanocluster.According to transmission electron microscopy (TEM), the size of the nanocluster ranged between 200 and 500 nm per cluster with 8 to 10 nm in diameter for each particle. Hydrodynamic diameter of the nanocluster significantly decreased as the dispersion temperature increased from 25 C to 45 C due to the shrinkage of thermo-responsive PNIPAAm when crossing its lower critical solution temperature (LCST). This stable nanocluster might be potentially used as a magnetic carrier for control release of entrapped entities with a thermally triggering mechanism. K E Y W O R D S magnetite, nanocluster, thermo-responsive, thiolactone, water dispersible

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Tunable temperature responsive liquid chromatography through thiolactone-based immobilization of poly(N-isopropylacrylamide)

Cited by 14 publications

References 34 publications

Enhancing the Possibilities of Comprehensive Two-Dimensional Liquid Chromatography through Hyphenation of Purely Aqueous Temperature-Responsive and Reversed-Phase Liquid Chromatography

Enhancing the Possibilities of Comprehensive Two-Dimensional Liquid Chromatography through Hyphenation of Purely Aqueous Temperature-Responsive and Reversed-Phase Liquid Chromatography

Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography

Water dispersible magnetite nanocluster coated with thermo‐responsive thiolactone‐containing copolymer

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