Tuneable peptide cross-linked nanogels for enzyme-triggered protein delivery

Massi, Lucia; Najer, Adrian; Chapman, Robert; Spicer, Christopher D.; Nele, Valeria; Che, Junyi; Booth, Marsilea A.; Doutch, James; Stevens, Molly M.

doi:10.1039/d0tb01546f

Cited by 23 publications

(21 citation statements)

References 61 publications

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“…The LCST can be tuned by adjusting the polymer component. The LCSTs of PNIPAM and PNCPAM are 32 and 49 °C, respectively 36 . P(NIPAM‐ co ‐NCPAM) obtained by copolymerization of NIPAM and NCPAM had an LCST ranging from 32 to 49 °C with the varied ratio of NIPAM and NCPAM.…”

Section: Thermoresponsive Nanogelsmentioning

confidence: 99%

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Bioresponsive nanogels for protein delivery

Shen

2022

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Protein drugs have attracted more attentions due to their high specificity and efficacy. However, the poor stability, plasma degradation, inferior cell membrane permeability, and immunogenicity severely limit the in vivo application of protein drugs. Nanogels are the nanosized crosslinked gels with high water‐loading capacities and large cavities for protein loading, which are able to increase the stability and decrease the immunogenicity for protein delivery. The bioresponsive nanogels possess the capability of programmatically releasing the protein drugs in an on‐demand manner at the target sites with distinct biosignals, which show considerable potential to increase the therapeutic efficacies and decrease the adverse effects of the protein drugs. In this review, we outline the recent advance in the bioresponsive nanogels for delivery of protein drugs, and survey the design of new materials and formulations that can respond typical biosignals, such as temperature, pH, reductive potential, and enzyme expression. The prospects and challenges are also discussed.

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Section: Thermoresponsive Nanogelsmentioning

confidence: 99%

“…Massi et al . synthesized P(NIPAM‐ co ‐NCPAM) with different monomer ratios and examined their physical properties 36 . The higher molar ratio of NIPAM and NCPAM, the higher phase transition temperature.…”

Section: Thermoresponsive Nanogelsmentioning

confidence: 99%

Bioresponsive nanogels for protein delivery

Shen

2022

VIEW

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“…These nanogels were highly dependent on glucose or pH concentrations, where low amounts of proteins were released in physiological pH or under healthy blood glucose level, while the rapid release was seen at lower pH or under diabetic blood glucose level [83]. Massi et al generated a temperature-sensitive nanogel that responded to matrix metalloproteinase-7 (MMP-7) and with the addition of N-cyclopropylacrylamide (NCPAM) into NIPAM-based copolymer, it allowed the encapsulation of protein cargo and nanogel-crosslinking at slightly elevated temperatures [84].…”

Section: Delivery Of Peptides or Proteins Using Stimuli-responsive Hydrogelsmentioning

confidence: 99%

Development of a Polysaccharide-Based Hydrogel Drug Delivery System (DDS): An Update

Pushpamalar

Meganathan

Tan

et al. 2021

Gels

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Delivering a drug to the target site with minimal-to-no off-target cytotoxicity is the major determinant for the success of disease therapy. While the therapeutic efficacy and cytotoxicity of the drug play the main roles, the use of a suitable drug delivery system (DDS) is important to protect the drug along the administration route and release it at the desired target site. Polysaccharides have been extensively studied as a biomaterial for DDS development due to their high biocompatibility. More usefully, polysaccharides can be crosslinked with various molecules such as micro/nanoparticles and hydrogels to form a modified DDS. According to IUPAC, hydrogel is defined as the structure and processing of sols, gels, networks and inorganic–organic hybrids. This 3D network which often consists of a hydrophilic polymer can drastically improve the physical and chemical properties of DDS to increase the biodegradability and bioavailability of the carrier drugs. The advancement of nanotechnology also allows the construction of hydrogel DDS with enhanced functionalities such as stimuli-responsiveness, target specificity, sustained drug release, and therapeutic efficacy. This review provides a current update on the use of hydrogel DDS derived from polysaccharide-based materials in delivering various therapeutic molecules and drugs. We also highlighted the factors that affect the efficacy of these DDS and the current challenges of developing them for clinical use.

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“…Most recently, CC-9 peptide conjugated to poly(acrylamide-co-methacrylic acid) nanogels showed enhanced co-localization with SW-48 colon cancer cell lines to augment colon cancer targeting abilities [103]. To date, various peptides (e.g., papain, albumin, arginine-glycine-aspartic acid (RGD), and azido-bi-functionalized peptides) have been used in the development of peptide-polymer conjugates [101,[104][105][106]. Studies reported that these peptide-polymer NPs provides new opportunities to generate hybrid NPs with attractive properties such as tumor targeting, stimuli-and microenvironmental responsiveness while minimizing off-target toxicity [101,104].…”

Section: Peptide-polymer Conjugatesmentioning

confidence: 99%

Development of Polymer-Assisted Nanoparticles and Nanogels for Cancer Therapy: An Update

Neerooa

Ooi

Shameli

et al. 2021

Gels

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With cancer remaining as one of the main causes of deaths worldwide, many studies are undergoing the effort to look for a novel and potent anticancer drug. Nanoparticles (NPs) are one of the rising fields in research for anticancer drug development. One of the key advantages of using NPs for cancer therapy is its high flexibility for modification, hence additional properties can be added to the NPs in order to improve its anticancer action. Polymer has attracted considerable attention to be used as a material to enhance the bioactivity of the NPs. Nanogels, which are NPs cross-linked with hydrophilic polymer network have also exhibited benefits in anticancer application. The characteristics of these nanomaterials include non-toxic, environment-friendly, and variable physiochemical properties. Some other unique properties of polymers are also attributed by diverse methods of polymer synthesis. This then contributes to the unique properties of the nanodrugs. This review article provides an in-depth update on the development of polymer-assisted NPs and nanogels for cancer therapy. Topics such as the synthesis, usage, and properties of the nanomaterials are discussed along with their mechanisms and functions in anticancer application. The advantages and limitations are also discussed in this article.

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Tuneable peptide cross-linked nanogels for enzyme-triggered protein delivery

Abstract: A tuneable peptide-crosslinked, temperature-sensitive nanogel platform for protein delivery in response to MMP-7, which is overexpressed in many pathologies, is presented.

Cited by 23 publications

References 61 publications

Bioresponsive nanogels for protein delivery

Bioresponsive nanogels for protein delivery

Development of a Polysaccharide-Based Hydrogel Drug Delivery System (DDS): An Update

Development of Polymer-Assisted Nanoparticles and Nanogels for Cancer Therapy: An Update

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