Tunicamycin and papulacandin B inhibit incorporation of specific mannoproteins into the wall of Candida albicans regenerating protoplasts

Murgui, Amelia; Mv, Elorza; Sentandreu, Rafael

doi:10.1016/0304-4165(86)90207-2

Cited by 48 publications

(30 citation statements)

References 17 publications

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“…After 3 h of regeneration, gold particles were uniformly distributed upon a material of low electron density and resembling wall ghosts. The latter seemed to be the very first material deposited on the surface of regenerating protoplasts, confirming previous analytical and cytological observations that indicated it to be chitin (Elorza et al, 1983;Murgui et al, 1986). This initial fibrillar skeleton retained mannoproteins from the very beginning Sentandreu et al, 1983), as some Con A-fe receptors were also clearly seen associated within the fibrillar structures (Fig.…”

Section: Ultrastructural Studiessupporting

confidence: 87%

“…Formation of a new wall by fungal protoplasts is initiated by deposition of a chitin skeleton which allows incorporation of other wall polymers (Elorza et al, 1983;Gibson et al, 1976;Murgui et al, 1986). This is interesting, as chitin is a minor component of the wall of normal growing cells, suggesting that the walls formed by protoplasts are initially abnormal structures, as previously reported (Netas & Svoboda, 1985).…”

Section: Discussionsupporting

confidence: 55%

“…Synthesis qf cell wall polymers by protoplasts The kinetics of incorporation of mannoproteins into the cell walls of regenerating protoplasts after incubation in the presence of [ U-' Tlprotein hydrolysate had been determined previously (Murgui et al, 1986), but it was of interest to learn how they and other polymers interacted once synthesized and before being incorporated into the walls. With this aim, protoplasts were incubated in an osmotically protected medium with [U-lSC]glucose as a precursor of polysaccharides and supplemented with papulacandin B, nikkomycin or tunicamycin.…”

Section: R E S U L T Smentioning

confidence: 99%

“…Chitin, the first polymer detected on the protoplast surface (Murgui et al, 1986), was incorporated normally whereas incorporation of the S-and I-glucans was significantly decreased (Table 1).…”

Section: R E S U L T Smentioning

confidence: 99%

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Formation of a New Cell Wall by Protoplasts of Candida albicans: Effect of Papulacandin B, Tunicamycin and Nikkomycin

Elorza

Murgui²,

Rico³

et al. 1987

Microbiology

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Incorporation of polysaccharides into the walls of regenerating protoplasts of Candida albicans was followed in the presence of papulacandin B, tunicamycin and nikkomycin. With the first drug, chitin was incorporated normally whereas incorporation of glucans and mannoproteins was significantly decreased. Tunicamycin decreased incorporation of all wall polymers when added at the beginning of the regeneration process but blocked only mannan and alkaliinsoluble glucan incorporation when added after 5 h. Nikkomycin inhibited chitin synthesis, and the walls formed by the protoplasts were enriched in alkali-soluble glucan. Pulse-chase experiments suggested that a precursor-product relationship between the alkali-soluble and alkali-insoluble glucans existed in the wall. The results obtained with the antibiotics were confirmed and extended by cytological studies using wheat-germ agglutinin labelled with colloidal gold and concanavalin A-ferri tin as specific markers of chitin and mannoproteins respectively. The results support the idea that regeneration of walls by protoplasts occurs in two steps : firstly, a chitin microfibrillar skeleton is formed, and in a later step glucan-mannoprotein complexes are added to the growing structure. The chitin skeleton probably allows the orderly spatial arrangement of the other polymers giving rise to the regenerated cell wall.

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Section: Ultrastructural Studiessupporting

confidence: 87%

Section: Discussionsupporting

confidence: 55%

Section: R E S U L T Smentioning

confidence: 99%

Section: R E S U L T Smentioning

confidence: 99%

See 2 more Smart Citations

Formation of a New Cell Wall by Protoplasts of Candida albicans: Effect of Papulacandin B, Tunicamycin and Nikkomycin

Elorza

Murgui²,

Rico³

et al. 1987

Microbiology

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“…The physiological significance of the very high polydispersity of the wall mannoproteins is totally unknown. The observations that (i) changes in a single sugar residue affect the overall conformation of a macromolecule, and (ii) the carbohydrate moiety of glycoconjugates changes during development, differentiation and transformation (Stanley, 1987), point to the important functional role of mannoproteins in controlling the cell wall structure (Murgui et al, 1986;Elorza et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Wall Mannoproteins of the Yeast and Mycelial Cells of Candida albicuns: Nature of the Glycosidic Bonds and Polydispersity of Their Mannan Moieties

Elorza

Marcilla²,

Sentandreu³

1988

Microbiology

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Zymolyase released between 20 and 25% of the total protein from purified walls of yeast (Y) and mycelial (M) cells of Candida albicans. The material released contained 92% carbohydrate (86% mannose and 6 % glucose) and 7 :< protein. Over 85 % of the carbohydrate was N-glycosidically linked to the protein and the rest (less than 15%) was linked 0-glycosidically. Highly polydisperse, high molecular mass mannoproteins, resolved by electrophoresis as four defined bands in Y cells and two bands in M cells, had both types of sugar chains. A 34 kDa species found in both types of cells had a single 2.5 kDa N-glycosidically linked sugar chain and a 3 1.5 kDa protein moiety. Polydispersity in the high molecular mass mannoproteins was due to the N-linked sugar chains (mannan) with a molecular mass between 500 kDa and 20 kDa (average 100 kDa) in Y cells and between 400 kDa and 20 kDa (average 50 kDa) in M cells.Three mannoproteins of 34,30 and 29 kDa secreted by protoplasts were associated with the high molecular mass mannoproteins, suggesting that this type of interaction might be related to the regeneration of the cell wall.

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Cell wall glucomannoproteins of Saccharomyces cerevisiae mnn9

Rinsum

Klis

Ende

1991

Yeast

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Mannoproteins were isolated from Saccharomyces cerevisiae mnn9 mutant cell walls by laminarinase digestion and purified by affinity and anion-exchange chromatography. The purified mannoprotein fraction contained three predominant proteins with molecular masses of 300 kDa, 220 kDa and 160 kDa. These compounds were absent in an SDS extract of cell walls or in a hot-citrate extract of mnn9 cells. The carbohydrate part of the purified mannoproteins consisted of (N-acetyl)glucosamine, mannose and glucose in a molar ratio of 1:53:4. O-Glycosidically linked chains, containing 70% of the mannose, were released by mild beta-elimination. N-Glycosidically linked chains, representing 80% of the (N-acetyl)glucosamine and 20% of the mannose, were released by peptide N-glycosidase F (PNGase F) digestion. Complete degradation of protein by alkaline hydrolysis released besides the N- and O-glycosidically linked chains, another type of carbohydrate chain containing the residual (N-acetyl)glucosamine, mannose and most of the glucose in a molar ratio of 1:17:18. Glucose was beta-glycosidically linked. The results indicate that beta-glucose is linked to PNGase F-resistant N-linked chains present on cell wall mannoproteins. We propose that these chains are responsible for the linkage between mannoproteins and glucan in the cell wall.

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Tunicamycin and papulacandin B inhibit incorporation of specific mannoproteins into the wall of Candida albicans regenerating protoplasts

Cited by 48 publications

References 17 publications

Formation of a New Cell Wall by Protoplasts of Candida albicans: Effect of Papulacandin B, Tunicamycin and Nikkomycin

Formation of a New Cell Wall by Protoplasts of Candida albicans: Effect of Papulacandin B, Tunicamycin and Nikkomycin

Wall Mannoproteins of the Yeast and Mycelial Cells of Candida albicuns: Nature of the Glycosidic Bonds and Polydispersity of Their Mannan Moieties

Cell wall glucomannoproteins of Saccharomyces cerevisiae mnn9

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