2018
DOI: 10.3390/ijms19124114
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Tunicamycin-Induced ER Stress is Accompanied with Oxidative Stress via Abrogation of Sulfur Amino Acids Metabolism in the Liver

Abstract: Endoplasmic reticulum (ER) stress is involved in non-alcoholic fatty liver disease (NAFLD), but the relationship between oxidative stress, another well-known risk factor of NAFLD, and ER stress has yet to be elucidated. In this study, we treated mice with tunicamycin (TM) (2 mg/kg body weight) for 48 h to induce ER stress in the liver and examined the metabolic pathway that synthesizes the endogenous antioxidant, glutathione (GSH). Tunicamycin (TM) treatment significantly increased mRNA levels of CHOP and GRP7… Show more

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Cited by 41 publications
(42 citation statements)
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“…Hepatic taurine was derivatized with o-phthalaldehyde/2-mercaptoethanol and quantified using an HPLC system equipped with a fluorescence detector (excitation 338 nm and emission 425 nm; FLD-3100, Thermo Scientific) [24] after separation using a Hector T-C18 column (3 µm × 4.6 mm × 100 mm; Chiral Technology Korea, Daejeon, Korea). The levels of cysteine and GSH were analyzed by the SBD-F derivatization method [25] using an HPLC equipped with a Hector M-C18 column (3 µm × 4.6 mm × 150 mm: Chiral Technology Korea) and a fluorescence detector (excitation 385 nm and emission 515 nm; FLD-3100, Thermo Scientific).…”
Section: Determination Of Levels Of Hepatic Cysteine Gsh and Taurinementioning
confidence: 99%
“…Hepatic taurine was derivatized with o-phthalaldehyde/2-mercaptoethanol and quantified using an HPLC system equipped with a fluorescence detector (excitation 338 nm and emission 425 nm; FLD-3100, Thermo Scientific) [24] after separation using a Hector T-C18 column (3 µm × 4.6 mm × 100 mm; Chiral Technology Korea, Daejeon, Korea). The levels of cysteine and GSH were analyzed by the SBD-F derivatization method [25] using an HPLC equipped with a Hector M-C18 column (3 µm × 4.6 mm × 150 mm: Chiral Technology Korea) and a fluorescence detector (excitation 385 nm and emission 515 nm; FLD-3100, Thermo Scientific).…”
Section: Determination Of Levels Of Hepatic Cysteine Gsh and Taurinementioning
confidence: 99%
“…Since NAFLD involves multiple metabolic pathways ( Fig. 1), more attention should be paid to abnormal metabolism that may worsen metabolic disorders (9)(10)(11)(12)(13). In the present review, the abnormal metabolism in NAFLD, including glucose, lipid, amino acids, bile acids and iron metabolism are summarized with the aim to provide evidence and hypotheses for NAFLD diagnosis and possible therapeutic strategies.…”
Section: Introductionmentioning
confidence: 99%
“…Isolation of total RNA using the Direct-zol™ RNA kit (Zymo Research, Orange, CA, USA) and synthesis of cDNA using the iScript™ cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA) followed the manufacturer's protocol. The transcripts were amplified using the SensiFAST SYBR qPCR mix (Bioline, London, UK) [31]. Table 1 shows the primers used for amplification.…”
Section: Real-time Reverse Transcription-polymerase Chain Reaction (Rmentioning
confidence: 99%