Tuning a Genetic Switch: Experimental Evolution and Natural Variation of Prophage Induction

Refardt, Dominik; Rainey, Paul B.

doi:10.1111/j.1558-5646.2009.00882.x

Cited by 42 publications

(41 citation statements)

References 65 publications

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“…One-sided experimental evolution of the filamentous phage f1 demonstrated increased virulence (in terms of larger impact on population density) when horizontal transmission among hosts was increased relative to vertical transmission within a dividing bacterial lineage (Messenger et al , 1999), but it remains to be determined whether the bacterial population would respond by evolving increased resistance under these same conditions. One-sided experimental evolution of the lysogenic phage λ was also used to select for altered sensitivity and threshold for the switch from lysogenic to lytic phage life cycle (Refardt & Rainey, 2010). …”

Section: Future Directionsmentioning

confidence: 99%

Bacteria–phage coevolution as a driver of ecological and evolutionary processes in microbial communities

Koskella¹,

Brockhurst²

2014

FEMS Microbiol Rev

704

557

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Bacteria–phage coevolution, the reciprocal evolution between bacterial hosts and the phages that infect them, is an important driver of ecological and evolutionary processes in microbial communities. There is growing evidence from both laboratory and natural populations that coevolution can maintain phenotypic and genetic diversity, increase the rate of bacterial and phage evolution and divergence, affect community structure, and shape the evolution of ecologically relevant bacterial traits. Although the study of bacteria–phage coevolution is still in its infancy, with open questions regarding the specificity of the interaction, the gene networks of coevolving partners, and the relative importance of the coevolving interaction in complex communities and environments, there have recently been major advancements in the field. In this review, we sum up our current understanding of bacteria–phage coevolution both in the laboratory and in nature, discuss recent findings on both the coevolutionary process itself and the impact of coevolution on bacterial phenotype, diversity and interactions with other species (particularly their eukaryotic hosts), and outline future directions for the field.

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Section: Future Directionsmentioning

confidence: 99%

Bacteria–phage coevolution as a driver of ecological and evolutionary processes in microbial communities

Koskella¹,

Brockhurst²

2014

FEMS Microbiol Rev

704

557

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“…The molecular switches controlling these life-history decisions are likely to be important targets for selection, indeed the lysis/lysogeny switch of phage lambda has been shown to evolve in the lab, [64] future work should focus on how key environmental drivers shape adaption of temperate phage life-history. 2.…”

Section: Future Directions -Unraveling the Evolutionary Ecology Of Tementioning

confidence: 99%

Ecological and Evolutionary Benefits of Temperate Phage: What Does or Doesn't Kill You Makes You Stronger

2017

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Infection by a temperate phage can lead to death of the bacterial cell, but sometimes these phages integrate into the bacterial chromosome, offering the potential for a more long-lasting relationship to be established. Here we define three major ecological and evolutionary benefits of temperate phage for bacteria: as agents of horizontal gene transfer (HGT), as sources of genetic variation for evolutionary innovation, and as weapons of bacterial competition. We suggest that a coevolutionary perspective is required to understand the roles of temperate phages in bacterial populations.

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“…Second, natural environments are more variable than the assay conditions of this study. For example, it is known that lambdoid prophages vary both in their sensitivity toward an inducing signal and in their response towards different signals (Łoś et al, 2009;Refardt and Rainey 2010). Finally, it is also conceivable that competitive ability depends on the host genotype (de Roode et al, 2004).…”

Section: Patterns Of Competitive Interactions Among Lambdoid Phagesmentioning

confidence: 99%

Within-host competition determines reproductive success of temperate bacteriophages

Refardt

2011

The ISME Journal

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Within-host competition between parasites is frequently invoked as a major force for parasite evolution, yet quantitative studies on its extent in an organismal group are lacking. Temperate bacteriophages are diverse and abundant parasites of bacteria, distinguished by their ability to enter a facultative dormant state in their host. Bacteria can accumulate multiple phages that may eventually abandon dormancy in response to host stress. Host resources are then converted into phage particles, whose release requires cell death. To study within-host competition between phages, I used the bacterium Escherichia coli and 11 lambdoid phages to construct single and double lysogens. Lysogenic bacterial cultures were then induced and time to host cell lysis and productivity of phages was measured. In double lysogens, this revealed strong competitive interactions as in all cases productivity of at least one phage declined. The outcome of within-host competition was often asymmetrical, and phages were found to vary hierarchically in within-host competitive ability. In double infections, the phage with the shorter lysis time determined the timing of cell lysis, which was associated with a competitive advantage when time differences were large. The results emphasize that within-host competition greatly affects phage fitness and that multiple infections should be considered an integral part of bacteriophage ecology.

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Tuning a Genetic Switch: Experimental Evolution and Natural Variation of Prophage Induction

Cited by 42 publications

References 65 publications

Bacteria–phage coevolution as a driver of ecological and evolutionary processes in microbial communities

Bacteria–phage coevolution as a driver of ecological and evolutionary processes in microbial communities

Ecological and Evolutionary Benefits of Temperate Phage: What Does or Doesn't Kill You Makes You Stronger

Within-host competition determines reproductive success of temperate bacteriophages

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