Tuning the electrophoretic separations on a surface-accessible and flexible fibre-based microfluidic devices

Khan, Jawairia Umar; Pathan, Mirbaz Ali; Sayyar, Sepidar; Paull, Brett; Innis, Peter C.

doi:10.1039/d2ay01714h

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…Textile-based electrofluidics is still in its infancy with the majority of studies being carried out on single yarn substrates [18,[30][31][32]. To date, there have been only limited reports on the application of more complex 3D textile structures [33][34][35]. Our research group recently reported the work on polyester braids as a 3D textile structure for electrofluidics [33].…”

Section: Introductionmentioning

confidence: 99%

Novel 3D textile structures and geometries for electrofluidics

Abeywardena,

Yue,

Wallace

et al. 2024

Electrophoresis

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The integration of microfluidics with electric field control, commonly referred to as electrofluidics, has led to new opportunities for biomedical analysis. The requirement for closed microcapillary channels in microfluidics, typically formed via complex microlithographic fabrication approaches, limits the direct accessibility to the separation processes during conventional electrofluidic devices. Textile structures provide an alternative and low‐cost approach to overcome these limitations via providing open and surface‐accessible capillary channels. Herein, we investigate the potential of different 3D textile structures for electrofluidics. In this study, 12 polyester yarns were braided around nylon monofilament cores of different diameters to produce functional 3D core–shell textile structures. Capillary electrophoresis performances of these 3D core–shell textile structures both before and after removing the nylon core were evaluated in terms of mobility and bandwidth of a fluorescence marker compound. It was shown that the fibre arrangement and density govern the inherent capillary formation within these textile structures which also impacts upon the solute analyte mobility and separation bandwidth during electrophoretic studies. Core–shell textile structures with a 0.47 mm nylon core exhibited the highest fluorescein mobility and presented a narrower separation bandwidth. This optimal textile structure was readily converted to different geometries via a simple heat‐setting of the central nylon core. This approach can be used to fabricate an array of miniaturized devices that possess many of the basic functionalities required in electrofluidics while maintaining open surface access that is otherwise impractical in classical approaches.

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Section: Introductionmentioning

confidence: 99%

Novel 3D textile structures and geometries for electrofluidics

Abeywardena,

Yue,

Wallace

et al. 2024

Electrophoresis

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“…Textile-based electrofluidics possess many advantages opening new opportunities in biomedical research applications [1][2][3][4][5]. In textile-based electrofluidics, thousands of capillary channels between yarns of textile facilitate active fluid flow with the help of electric field, diminishing the limitations associated with passive flow [6].…”

Section: Introductionmentioning

confidence: 99%

Electrofluidic control for textile‐based cell culture: Identification of appropriate conditions required to integrate cell culture with electrofluidics

Abeywardena,

Yue,

Wallace

et al. 2024

Electrophoresis

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Electric field–driven microfluidics, known as electrofluidics, is a novel attractive analytical tool when it is integrated with low‐cost textile substrate. Textile‐based electrofluidics, primarily explored on yarn substrates, is in its early stages, with few studies on 3D structures. Further, textile structures have rarely been used in cellular analysis as a low‐cost alternative. Herein, we investigated novel 3D textile structures and develop optimal electrophoretic designs and conditions that are favourable for direct 3D cell culture integration, developing an integrated cell culture textile‐based electrofluidic platform that was optimised to balance electrokinetic performance and cell viability requirements. Significantly, there were contrasting electrolyte compositional conditions that were required to satisfy cell viability and electrophoretic mobility requiring the development of and electrolyte that satisfied the minimum requirements of both these components within the one platform. Human dermal fibroblast cell cultures were successfully integrated with gelatine methacryloyl (GelMA) hydrogel‐coated electrofluidic platform and studied under different electric fields using 5 mM TRIS/HEPES/300 mM glucose. Higher analyte mobility was observed on 2.5% GelMA‐coated textile which also facilitated excellent cell attachment, viability and proliferation. Cell viability also increased by decreasing the magnitude and time duration of applied electric field with good cell viability at field of up to 20 V cm−1.

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Optimisation of supercritical fluid extraction of orange (Citrus sinenis L.) peel essential oil and its physicochemical properties

Ling Felicia,

Rovina,

Nur Aqilah

et al. 2024

Current Research in Green and Sustainable Chemistry

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Tuning the electrophoretic separations on a surface-accessible and flexible fibre-based microfluidic devices

Abstract: Electrophoretic separation of charged ions on a textile-braided structure.

Cited by 4 publications

References 36 publications

Novel 3D textile structures and geometries for electrofluidics

Novel 3D textile structures and geometries for electrofluidics

Electrofluidic control for textile‐based cell culture: Identification of appropriate conditions required to integrate cell culture with electrofluidics

Optimisation of supercritical fluid extraction of orange (Citrus sinenis L.) peel essential oil and its physicochemical properties

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