Tuning the Mechanical Properties of a DNA Hydrogel in Three Phases Based on ATP Aptamer

Liu, Hengyuan; Cao, Tianyang; Xu, Yun‐Xiang; Dong, Yuanchen

doi:10.3390/ijms19061633

Cited by 36 publications

(41 citation statements)

References 38 publications

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“…For instance, Liu et al used ATP binding aptamers to control the mechanical strength of their DNA hydrogels. They showed that in the presence of ATP their hydrogels exhibit a G' of 380 Pa and a G' of 204 Pa in the absence of ATP [33]. Moreover, they demonstrated the ATP concentration dependence of their system that allows for fine-tuning of the hydrogel's mechanical properties.…”

Section: Nucleic Acid Structural Motifsmentioning

confidence: 99%

“…While DNA building blocks have been used extensively to assemble several nanoscale objects, DNA as a material is now gaining more traction as a potential candidate for larger scale polymeric biomaterials assembly, particularly to build nanostructured functional soft hydrogels with sizes ranging from the nanoscale to the millimeter scale. Similar to the discrete DNA nanostructures, the structural, mechanical, and biochemical properties of these DNA hydrogels are highly controllable [31][32][33]. In the past decade, several studies have demonstrated the potential of these DNA hydrogels to be used in several biomedical applications [34] including drug delivery [35][36][37], cancer therapy [38][39][40], antigen delivery and immunomodulation [41,42], sensing [43][44][45], and extracellular matrix (ECM) mimics for tissue engineering [34,46].…”

Section: Introductionmentioning

confidence: 99%

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Mechanical Properties of DNA Hydrogels: Towards Highly Programmable Biomaterials

Bush

Veneziano

2021

Applied Sciences

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DNA hydrogels are self-assembled biomaterials that rely on Watson–Crick base pairing to form large-scale programmable three-dimensional networks of nanostructured DNA components. The unique mechanical and biochemical properties of DNA, along with its biocompatibility, make it a suitable material for the assembly of hydrogels with controllable mechanical properties and composition that could be used in several biomedical applications, including the design of novel multifunctional biomaterials. Numerous studies that have recently emerged, demonstrate the assembly of functional DNA hydrogels that are responsive to stimuli such as pH, light, temperature, biomolecules, and programmable strand-displacement reaction cascades. Recent studies have investigated the role of different factors such as linker flexibility, functionality, and chemical crosslinking on the macroscale mechanical properties of DNA hydrogels. In this review, we present the existing data and methods regarding the mechanical design of pure DNA hydrogels and hybrid DNA hydrogels, and their use as hydrogels for cell culture. The aim of this review is to facilitate further study and development of DNA hydrogels towards utilizing their full potential as multifeatured and highly programmable biomaterials with controlled mechanical properties.

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Section: Nucleic Acid Structural Motifsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanical Properties of DNA Hydrogels: Towards Highly Programmable Biomaterials

Bush

Veneziano

2021

Applied Sciences

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“…Beyond self-assembly, DNA is also biocompatible and can be readily conjugated with other bio-/nanomaterials including proteins and conductive polymers [6][7][8][9]. Leveraging these capabilities, DNA-based hydrogels have drawn a lot of attention starting with basic research and moving to applications such as biomedicine, biosensing, and drug delivery [10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Mechanical and Electrical Properties of DNA Hydrogel-Based Composites Containing Self-Assembled Three-Dimensional Nanocircuits

et al. 2021

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Molecular self-assembly of DNA has been developed as an effective construction strategy for building complex materials. Among them, DNA hydrogels are known for their simple fabrication process and their tunable properties. In this study, we have engineered, built, and characterized a variety of pure DNA hydrogels using DNA tile-based crosslinkers and different sizes of linear DNA spacers, as well as DNA hydrogel/nanomaterial composites using DNA/nanomaterial conjugates with carbon nanotubes and gold nanoparticles as crosslinkers. We demonstrate the ability of this system to self-assemble into three-dimensional percolating networks when carbon nanotubes and gold nanoparticles are incorporated into the DNA hydrogel. These hydrogel composites showed interesting non-linear electrical properties. We also demonstrate the tuning of rheological properties of hydrogel-based composites using different types of crosslinkers and spacers. The viscoelasticity of DNA hydrogels is shown to dramatically increase by the use of a combination of interlocking DNA tiles and DNA/carbon nanotube crosslinkers. Finally, we present measurements and discuss electrically conductive nanomaterials for applications in nanoelectronics.

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“…Recently, stimuli‐responsive DNA hydrogels have also been designed and prepared by taking advantage of the predictable hybridization of DNAs, along with unique DNA sequences (i‐motif, G‐quadruplex, and triplex), functionalized DNAs, or aptamers. They exhibited a phase transition from the gel state to sol state in response to various stimuli such as heat, pH, light, metal ions, and biomolecules (nucleic acids, proteins, enzymes, and small molecules) . In particular, biological stimuli‐responsive DNA hydrogels have successfully been applied for drug‐delivery systems, cell engineering, and biosensing systems .…”

Section: Introductionmentioning

confidence: 99%

Dynamically Programmed Switchable DNA Hydrogels Based on a DNA Circuit Mechanism

Oishi

Nakatani

2019

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Biological stimuli‐responsive DNA hydrogels have attracted much attention in the field of medical engineering owing to their unique phase transitions from gel to sol through cleavage of DNA cross‐linking points in response to specific biomolecular inputs. In this paper, a new class of biological stimuli‐responsive DNA hydrogels with a dynamically programmed DNA system that relies on a DNA circuit system through cascading toehold‐mediated DNA displacement reactions is constructed, allowing the catalytic cleavage of cross‐linking points and main chains in response to an appropriate DNA input. The dynamically programmed DNA hydrogels exhibit a significant sharp phase transition from gel to sol in comparison to another DNA hydrogel showing noncatalytic cleavage of cross‐linking points due to synchronization of the catalytic cleavage of cross‐linking points and the main chains. Further, the sol–gel phase transitions of the DNA hydrogels in response to the DNA input are easily tunable by changing the cross‐linking density. Additionally, with a structure‐switching aptamer, DNA hydrogels encapsulating PEGylated gold nanoparticles can be used as enzyme‐free signal amplifiers for the colorimetric detection of adenosine 5′‐triphosphate (ATP); this detection system provides simplicity and higher sensitivity (limit of detection: 5.6 × 10−6 m at 30 min) compared to other DNA hydrogel‐based ATP detection systems.

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Tuning the Mechanical Properties of a DNA Hydrogel in Three Phases Based on ATP Aptamer

Cited by 36 publications

References 38 publications

Mechanical Properties of DNA Hydrogels: Towards Highly Programmable Biomaterials

Mechanical Properties of DNA Hydrogels: Towards Highly Programmable Biomaterials

Mechanical and Electrical Properties of DNA Hydrogel-Based Composites Containing Self-Assembled Three-Dimensional Nanocircuits

Dynamically Programmed Switchable DNA Hydrogels Based on a DNA Circuit Mechanism

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