Tunisian fig (<i>Ficus carica</i>L.) genetic diversity and cultivar characterization using microsatellite markers

Saddoud, Olfa; Salhi-Hannachi, Amel; Chatti, K.; Mars, Messaoud; Rhouma, Abdelmajid; Marrakchi, M.; Trifi, Mokhtar

doi:10.1051/fruits:2005018

Cited by 25 publications

(14 citation statements)

References 34 publications

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“…The PIC standards were used to evaluate the discrimi- The SSR method also has advantages such as high polymorphism, co-dominance, and reproducibility [37]. In this study, using 16 SSR markers, we observed 25 polymorphic alleles, with an average of 1.56 polymorphic alleles per primer, which was lower than the average values reported by Caliskan et al [4] (6.8), Aradhya et al [10] (4.9), Saddoud et al [38] (6.6), Ikegami et al [39] (5.2), Perez-Jiménez et al [40] (3.6), and Ganopoulos et al [41] (13). One of the disadvantages of using SSR markers is the high cost of designing the primers, which limits the number of species that can be assayed [42].…”

Section: Discussioncontrasting

confidence: 54%

Determination of the Population Structure of Fig Genotypes from Algeria and Turkey Using Inter Primer Binding Site-Retrotransposon and Simple Sequence Repeat Markers

Belttar¹,

Yahia²,

Nemli³

et al. 2017

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In order to identify the variation and estimate the genetic diversity among the fig (Ficus carica L.) genotypes collected from Algeria and Turkey, the genetic relationships between 86 genotypes were investigated using 23 inter primer binding sites (iPBS)-retrotransposon and 16 simple sequence repeat (SSR) primers. A total of 63 polymorphic bands for the iPBS-retrotransposon markers and 25 alleles for the SSR markers were identified with an average of 2.7 and 1.6 per primer, respectively. The average value of polymorphism information content (PIC) for the iPBS markers (0.73) was higher than that for the SSR markers (0.69). Applying the neighbor-joining method to the combined iPBS-retrotransposon and SSR data, the fig genotypes were clustered into two groups. The STRUCTURE software was used to determine the population structure. Among the genotypes studied, two populations (K = 2) were identified indicating a low diversity between the Algerian and Turkish varieties. Both types of markers were able to differentiate all the fig genotypes and were efficient in discriminating the closely related genotypes. Our data also showed that as a universal marker, iPBS-retrotransposon is a useful tool for the molecular characterization of fig genotypes.How to cite this paper: Belttar, H., Yahia,

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Section: Discussioncontrasting

confidence: 54%

Determination of the Population Structure of Fig Genotypes from Algeria and Turkey Using Inter Primer Binding Site-Retrotransposon and Simple Sequence Repeat Markers

Belttar¹,

Yahia²,

Nemli³

et al. 2017

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“…All produced clear electrophoretic profiles, uncovering a high level of polymorphism in fig varieties. Amplification and detection were performed following Saddoud et al (2005). The amplified banding patterns were firstly checked on 2% agarose gels visualised with ethidium bromide staining under UV light.…”

Section: Ssr Analysismentioning

confidence: 99%

Comparative Assessment of SSR and AFLP Markers for Evaluation of Genetic Diversity and Conservation of Fig, Ficus carica L., Genetic Resources in Tunisia

et al. 2010

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This study characterises the genetic variability of fig, Ficus carica L., using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. It compares the efficiency and utility of the two techniques in detecting variation and establishing genetic relationships among Tunisian fig cultivars.Our results show that using both marker systems, the Tunisian fig germ plasm is characterised by having a large genetic diversity at the deoxyribonucleic acid level, as most of AFLP bands were detected and all SSR markers were polymorphic. In fact, 351 (342 polymorphic) and 57 (57 polymorphic) bands were detected using AFLP and SSR primers, respectively. SSR markers were the most polymorphic with an average polymorphic information content value of 0.94, while AFLP markers showed the highest effective multiplex ratio (56.9) and marker index (45.2). The effective marker index was recorded highest (4.19) for AFLP markers and lowest (0.70) for the SSR ones. Our results demonstrate that (1) independent as well as combined analyses of cluster analyses of SSR and AFLP fragments showed that cultivars are clustered independently from their geographical origin, horticultural classifications and tree sex; (2) the analysis of molecular variance allowed the partitioning of genetic variation within and among fig groups and showed greater variation within groups and (3) AFLP and SSR markers datasets showed positive correlation. This study suggests the SSR and AFLP markers are suitable for diversity analysis and cultivars fingerprinting. An understanding of the genetic diversity and population structure of F. carica in Tunisia can also provide insight into the conservation and management of this species.

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“…The common fig ( Ficus carica L.) is a temperate species native to south‐west Asia and the Eastern Mediterranean region (Saddoud et al. 2005).…”

Section: Introductionmentioning

confidence: 99%

Current Status of Fig Mosaic Disease in Iran

Shahmirzaie

Rakhshandehroo

Zamanizadeh

et al. 2012

Journal of Phytopathology

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During 2009–2010, a survey was conducted in gardens and commercial fig orchards throughout Iran to determine the prevalence of Fig leaf mottle‐associated virus 1 (FLMaV‐1), Fig leaf mottle‐associated virus 2 (FLMaV‐2), Fig mild mottle‐associated virus (FMMaV), Fig latent virus 1 (FLV‐1) and Fig mosaic virus (FMV). Reverse transcription‐polymerase chain reaction and dot immunobinding assay (DIBA) were conducted on 104 fig samples collected from seven provinces. FLV‐1, FLMaV‐1 and FMV were found in 14.5, 11.5 and 8.6% of the samples, respectively, but FLMaV‐2 and FMMaV were absent. The overall average of infection reached 18.3%, with a peak of 42.9% in Semnan Province, followed by Golestan (40%), Tehran (32.3%), Lorestan (28.6%) and Mazandaran (25%) provinces. No infection was found in Fars and Gilan provinces. Fig samples from Varamin and Khorramabad districts showed high levels of mixed infections, 35.7 and 28.6%, respectively. The presence of FMV and FLV‐1 in the sap of symptomatic fig leaves was also ascertained by DIBA. Sequence analysis of amplified DNA from the partial RNA‐dependent RNA polymerase gene of two FMV isolates from Iran showed a low level of nucleotide variability (5%). The Iranian isolates shared a common phylogeny with other Mediterranean FMV isolates and in particular with those originating from Turkey already reported in GenBank. This is the first report on the presence of FLMaV‐1 and FLV‐1 in Iran and offers a preliminary insight into the unsatisfactory health status of fig in this country.

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Tunisian fig (Ficus caricaL.) genetic diversity and cultivar characterization using microsatellite markers

Cited by 25 publications

References 34 publications

Determination of the Population Structure of Fig Genotypes from Algeria and Turkey Using Inter Primer Binding Site-Retrotransposon and Simple Sequence Repeat Markers

Determination of the Population Structure of Fig Genotypes from Algeria and Turkey Using Inter Primer Binding Site-Retrotransposon and Simple Sequence Repeat Markers

Comparative Assessment of SSR and AFLP Markers for Evaluation of Genetic Diversity and Conservation of Fig, Ficus carica L., Genetic Resources in Tunisia

Current Status of Fig Mosaic Disease in Iran

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