Turbidimetric analysis of growth kinetics of bacteria in the laboratory environment using smartphone

Hatiboruah, Diganta; Devi, Damayanti Y.; Namsa, Nima D.; Nath, Pabitra

doi:10.1002/jbio.201960159

Cited by 18 publications

(3 citation statements)

References 33 publications

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“…Regarding turbidity in kombucha analog beverages, this is associated with microbial growth. Komagataeibacter , predominant in the initial culture, induces cellulose production, thus exerting a direct influence on turbidity [ 85 , 86 ].…”

Section: Resultsmentioning

confidence: 99%

Advances in the Production of Sustainable Bacterial Nanocellulose from Banana Leaves

Dáger-López,

Chenché,

Ricaurte-Párraga

et al. 2024

Polymers

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Interest in bacterial nanocellulose (BNC) has grown due to its purity, mechanical properties, and biological compatibility. To address the need for alternative carbon sources in the industrial production of BNC, this study focuses on banana leaves, discarded during harvesting, as a valuable source. Banana midrib juice, rich in nutrients and reducing sugars, is identified as a potential carbon source. An optimal culture medium was designed using a simplex-centroid mixing design and evaluated in a 10 L bioreactor. Techniques such as Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA) were used to characterize the structural, thermal, and morphological properties of BNC. Banana midrib juice exhibited specific properties, such as pH (5.64), reducing sugars (15.97 g/L), Trolox (45.07 µM), °Brix (4.00), and antioxidant activity (71% DPPH). The model achieved a 99.97% R-adjusted yield of 6.82 g BNC/L. Physicochemical analyses revealed distinctive attributes associated with BNC. This approach optimizes BNC production and emphasizes the banana midrib as a circular solution for BNC production, promoting sustainability in banana farming and contributing to the sustainable development goals.

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Section: Resultsmentioning

confidence: 99%

Advances in the Production of Sustainable Bacterial Nanocellulose from Banana Leaves

Dáger-López,

Chenché,

Ricaurte-Párraga

et al. 2024

Polymers

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“…Applied to the monitoring of a cell therapy product, measuring the optical density spectrum allows to distinguish between an increase in optical density due to cell growth or an increase due to contamination [19]. Recent works have also shown the possibility of developing portable systems suitable for in situ measurements [20,21].…”

Section: Turbidimetrymentioning

confidence: 99%

Technique Evolutions for Microorganism Detection in Complex Samples: A Review

et al. 2022

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Rapid detection of microorganisms is a major challenge in the medical and industrial sectors. In a pharmaceutical laboratory, contamination of medical products may lead to severe health risks for patients, such as sepsis. In the specific case of advanced therapy medicinal products, contamination must be detected as early as possible to avoid late production stop and unnecessary costs. Unfortunately, the conventional methods used to detect microorganisms are based on time-consuming and labor-intensive approaches. Therefore, it is important to find new tools to detect microorganisms in a shorter time frame. This review sums up the current methods and represents the evolution in techniques for microorganism detection. First, there is a focus on promising ligands, such as aptamers and antimicrobial peptides, cheaper to produce and with a broader spectrum of detection. Then, we describe methods achieving low limits of detection, thanks to Raman spectroscopy or precise handling of samples through microfluids devices. The last part is dedicated to techniques in real-time, such as surface plasmon resonance, preventing the risk of contamination. Detection of pathogens in complex biological fluids remains a scientific challenge, and this review points toward important areas for future research.

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“…In the present study, we have designed, developed, tested, and validated a smartphone-based spectroscopic device on a variety of cervical precancer and cancer patients at various hospitals in the Uttar Pradesh and Odisha states of India. Smartphone is useful in rapid data collection and image processing part [41][42][43][44][45][46]. A mutual information (MI)-based feature selection technique is employed to identify significant features from the initial spectral data, and subsequently a long short-term memory (LSTM) model is applied for classification purposes.…”

Section: Introductionmentioning

confidence: 99%

A smartphone‐based standalone fluorescence spectroscopy tool for cervical precancer diagnosis in clinical conditions

Shukla,

Deo,

Vishwakarma

et al. 2024

Journal of Biophotonics

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Real‐time prediction about the severity of noncommunicable diseases like cancers is a boon for early diagnosis and timely cure. Optical techniques due to their minimally invasive nature provide better alternatives in this context than the conventional techniques. The present study talks about a standalone, field portable smartphone‐based device which can classify different grades of cervical cancer on the basis of the spectral differences captured in their intrinsic fluorescence spectra with the help of AI/ML technique. In this study, a total number of 75 patients and volunteers, from hospitals at different geographical locations of India, have been tested and classified with this device. A classification approach employing a hybrid mutual information long short‐term memory model has been applied to categorize various subject groups, resulting in an average accuracy, specificity, and sensitivity of 96.56%, 96.76%, and 94.37%, respectively using 10‐fold cross‐validation. This exploratory study demonstrates the potential of combining smartphone‐based technology with fluorescence spectroscopy and artificial intelligence as a diagnostic screening approach which could enhance the detection and screening of cervical cancer.

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Turbidimetric analysis of growth kinetics of bacteria in the laboratory environment using smartphone

Cited by 18 publications

References 33 publications

Advances in the Production of Sustainable Bacterial Nanocellulose from Banana Leaves

Advances in the Production of Sustainable Bacterial Nanocellulose from Banana Leaves

Technique Evolutions for Microorganism Detection in Complex Samples: A Review

A smartphone‐based standalone fluorescence spectroscopy tool for cervical precancer diagnosis in clinical conditions

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