Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles

Wei, Ren; Oeser, Thorsten; Barth, Markus; Weigl, Nancy; Lübs, Anja; Schulz‐Siegmund, Michaela; Hacker, Michael C.; Zimmermann, Wolfgang

doi:10.1016/j.molcatb.2013.08.010

Cited by 87 publications

(88 citation statements)

References 40 publications

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“…The main compounds identified by comparison with the retention times of standards following their separation by HPLC were TPA, MHET and BHET. The maximum concentration of soluble products was detected after 1 h of reaction with TfCut2 when a complete degradation of the insoluble nanoparticles was observed [24]. MHET was found as the main product released accounting for 75% of the total hydrolysis products corresponding to 0.55 g MHET per g PET.…”

Section: Pet Hydrolysis Product Formation By Tfcut2mentioning

confidence: 94%

“…The total surface area of the PET nanoparticles was calculated based on their size determined by dynamic light scattering [24]. The particles showed a mean diameter of 100 to 160 nm [24,38].…”

Section: Preparation Of the Pet Nanoparticlesmentioning

confidence: 99%

“…TPA and BHET was detected in smaller amounts the reaction mixtures. The complete hydrolysis of the intermediate products MHET and BHET to TPA required a reaction time of approximately 24 h, significantly longer than the time needed to dissolve the insoluble PET nanoparticles [24].…”

Section: Pet Hydrolysis Product Formation By Tfcut2mentioning

confidence: 99%

“…PET nanoparticles provide a high surface to volume ratio and are much more readily hydrolyzed compared to films or fibers [23]. It has also been shown that the enzymatic hydrolysis of polyester materials is strongly depending on the mobility of the polymer chains determined by the crystallinity of the material [22,24]. The enzymatic degradation of PET is a heterogeneous catalytic process in some aspects similar to the hydrolysis of cellulose by cellulases [25].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of hydrolysis products on the enzymatic degradation of polyethylene terephthalate nanoparticles by a polyester hydrolase from Thermobifida fusca

Barth

Oeser

Wei

et al. 2015

Biochemical Engineering Journal

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190

167

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Section: Pet Hydrolysis Product Formation By Tfcut2mentioning

confidence: 94%

Section: Preparation Of the Pet Nanoparticlesmentioning

confidence: 99%

Section: Pet Hydrolysis Product Formation By Tfcut2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of hydrolysis products on the enzymatic degradation of polyethylene terephthalate nanoparticles by a polyester hydrolase from Thermobifida fusca

Barth

Oeser

Wei

et al. 2015

Biochemical Engineering Journal

Self Cite

190

167

View full text Add to dashboard Cite

“…4 This is usually monitored by high-performance liquid chromatography (HPLC), which separates monomeric TPA from its ethylene glycol esters, namely mono(2-hydroxyethyl) terephthalate (MHET) and bis-(2-hydroxyethyl) terephthalate (BHET), among other hydrolysis products. Additionally, a number of approaches to evaluate the extent of biodegradation have been used, including turbidimetric analysis, 10 titration, 11 image scanning, 12 and polymer film weight loss monitoring. 7 Fluorometric TPA detection has also been used to monitor PET degradation, but only for isolated enzymes.…”

Section: Introductionmentioning

confidence: 99%

A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms

Chaves¹,

Lima²,

Malafatti-Picca³

et al. 2017

J. Braz. Chem. Soc.

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We propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS).

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Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures

et al. 2019

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Polyethylene terephthalate (PET) is the most important mass‐produced thermoplastic polyester used as a packaging material. Recently, thermophilic polyester hydrolases such as TfCut2 from Thermobifida fusca have emerged as promising biocatalysts for an eco‐friendly PET recycling process. In this study, postconsumer PET food packaging containers are treated with TfCut2 and show weight losses of more than 50% after 96 h of incubation at 70 °C. Differential scanning calorimetry analysis indicates that the high linear degradation rates observed in the first 72 h of incubation is due to the high hydrolysis susceptibility of the mobile amorphous fraction (MAF) of PET. The physical aging process of PET occurring at 70 °C is shown to gradually convert MAF to polymer microstructures with limited accessibility to enzymatic hydrolysis. Analysis of the chain‐length distribution of degraded PET by nuclear magnetic resonance spectroscopy reveals that MAF is rapidly hydrolyzed via a combinatorial exo‐ and endo‐type degradation mechanism whereas the remaining PET microstructures are slowly degraded only by endo‐type chain scission causing no detectable weight loss. Hence, efficient thermostable biocatalysts are required to overcome the competitive physical aging process for the complete degradation of postconsumer PET materials close to the glass transition temperature of PET.

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Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles

Cited by 87 publications

References 40 publications

Effect of hydrolysis products on the enzymatic degradation of polyethylene terephthalate nanoparticles by a polyester hydrolase from Thermobifida fusca

Effect of hydrolysis products on the enzymatic degradation of polyethylene terephthalate nanoparticles by a polyester hydrolase from Thermobifida fusca

A Practical Fluorescence-Based Screening Protocol for Polyethylene Terephthalate Degrading Microorganisms

Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures

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