The aim of this study was to investigate the influence of humic substances (HS) on volumetric oxygen-transfer coefficient (K L a) under different turbulence intensities at the air-water interface. The experiments were carried out in an oscillating grid tank which provided three levels of turbulence intensities (Reynolds numbers of 5,116, 10,316, and 15,433). For each turbulence level, 15 different HS concentrations were tested and the K L a was estimated. The results showed that, regardless of the turbulence level in the tank, HS reduces K L a. The maximum reduction was 17%. The barrier effect, produced by the occupation of HS molecules at the interface, was the main phenomenon responsible for the reduction in the oxygen transfer. A relationship between the oxygen transfer velocity and turbulent kinetic energy showed that in the presence of HS, the air-water interface behaves like an immobile surface, reducing the oxygen diffusion and surface renewal.gas-water interface, grid-stirred tank, humic substances, oxygen mass transfer, reaeration