Abstract. Turbulence parameters in the tropo-stratosphere are analyzed using high-resolution balloon temperature measurements collected during the MUTSI (MU radar, Temperature sheets and Interferometry) campaign which took place near the Middle and Upper atmosphere (MU) radar (Japan, 35 • N, 136 • E) in May 2000. Vertical profiles of the specific dissipation rate of turbulent kinetic energy, ε, and turbulent diffusivity, K, are estimated from the Thorpe lengthscale, L T . The last is obtained by using two methods. The first one consists of measuring directly L T by reordering the potential temperature profiles. The second method is based on estimates of the temperature structure constant, C 2 T . A relationship between L T and C 2 T can be found by assuming either adiabatic vertical displacements or a model based on turbulent energy balance consideration. Analysis shows that the adiabatic assumption gives indirect estimates of L T more consistent with direct measurements. We also found that vertical profiles of analyzed turbulence characteristics show substantial intermittency, leading to substantial scatter of the local, median and average values. General trends correspond to a decrease in ε and K from the boundary layer up to altitudes 20-25 km. Layers of increased turbulence are systematically observed in the tropo-stratosphere, which may be produced by instabilities of temperature and wind profiles. These maxima may substantially increase local values of turbulence diffusivity.