In the atmospheric surface layer (ASL), the anisotropic characteristics of turbulence become more complex due to the strong influence of heat flux and particle motion. In this paper, the effects of stratification stability and total saltation sand flux on turbulent anisotropy of ASL wall turbulence are systematically analyzed by using high-frequency 3D velocity, temperature, and saltation sand flux measured in the field. Our results show that the temperature gradient intensifies the spanwise turbulent motion and enhances the vertical motion asymmetry. Although the buoyancy-driven turbulence enhances the spanwise variation, the momentum transfer mode between the streamwise–vertical and spanwise–vertical does not change. With the increase in instability, the large-scale motions (LSM) of the spanwise velocity may be destroyed and more spanwise small-scale motions (SSM) formed. In addition, saltation increases the sharpness, randomness, and extreme frequency of spanwise velocity distribution, but does not change the momentum transfer mode. Saltation may enlarge the fluctuation range and scale of the spanwise velocity, but may not be the main factor affecting the anisotropy of SSM. These results can deepen people's understanding of wind-blown sand movement and turbulence properties in ASL, and provides empirical data and insights that can significantly contribute to the development and refinement of turbulence models, particularly those that need to account for the complex interplay between stratification stability, saltation sand flux, and turbulence anisotropy.