Dissolved oxygen (DO) distribution at the sediment-water interface of a flow over a smooth bed is investigated for Reynolds numbers Ͼ360 and Ͻ4,090. These conditions are commonly encountered in streams, wetlands, and lakes. A power-law scaling of DO distribution is derived and compared with experimental data. The scaling analysis is based on DO flux at the sediment-water interface in a turbulent flow. The power-law model with diffusive sublayer thickness (DSLT) as a fitting parameter agrees well with the data over the investigated range of Reynolds numbers. Using the proposed power-law model with a limited number of DO and flow properties away from the sediment-water interface provides the distribution of DO concentrations and corresponding DSLT at a submillimeter resolution. The estimate of DSLT is, on average, 30% lower than the traditional estimate, defined as a thin fluid layer bounded at the lower boundary by a sediment bed and extended upward in the main water column to where a bulk DO concentration intersects with a linear DO gradient at the bed.The transport processes across the sediment-water interface are of fundamental importance to biological and chemical processes in streams, rivers, and lakes (Boudreau and Jørgensen 2001). In most cases, researchers are concerned about quantifying the distribution and corresponding flux of a particular substance at the sediment-water interface. The dissolved oxygen (DO) concentration in water has been considered one of the most important ecological parameters determining the water quality and associated biological composition of aquatic environments. The sediments, being a repository for decaying biological material with a large organic content, are a major contributor to the DO reduction in the water column.Significant laboratory and field measurements have been devoted to addressing the DO transport process at the sediment-water interface (e.g., Jørgensen and Des Marais 1990; Mackenthun and Stefan 1998;Røy et al. 2002). Microstructure DO measurements with microsensors (e.g., Jørgensen and Revsbech 1985;Lorke et al. 2003;Røy et al. 2004) have been very instrumental in the advancement of theories and models fundamental to the DO transport at the sedimentwater interface. Knowledge of the characteristics of the diffusive sublayer thickness (DSLT) for the DO transport and corresponding DO distribution at the sediment-water interface is crucial in benthic ecology. The difficulty in the characterization of the diffusive sublayer lies in its thinness, usually Ͻ5 mm, and the proximity of a solid boundary.