Türk Toplumunda GSTM1, GSTP1 ve GSTT1 Gen Polimorfizmlerinin Prostat Kanseri Riski Üzerine Etkileri

Abstract

We analysed glutathione S-transferase M1, T1, and P1 gene polymorphisms in a case control study to asses their susceptibility to prostate cancer in Turkish population. Polymorphisms were determined by PCR-based methods in 115 control subjects and 115 prostate cancer patients. The frequencies of polymorphisms were compared between patients and controls by logistic regression analysis. Possible associations of genetic profiles with respect to the Gleason score and patient age were also investigated. Besides GSTT1 null genotype has slightly increased prostate cancer risk (odds ratio(OR) = 1.50, 95% confidence interval (CI), 0.85-2.66), its association with prostate cancer risk was statistically significant among smokers (OR = 3.20, 95%CI, 1.06-9.70). GSTP1 Val/Val genotype was more common in cancer patients (OR = 1.63, 95%CI, 0.61-4.37). However, the combination of GSTP1 Ile/Ile and GSTT1 null genotype was associated with a higher risk (OR = 2.67, 95%CI, 1.00-7.10). Our results suggest that GSTT1 null genotype may modify prostate cancer risk among cigarette smokers seperately, and in combination with GSTP1 Ile/Ile genotype, irrespective of the smoking habit in Turkish population.Keywords: Prostate cancer, GSTM1, GSTP1, GSTT1, Polymorphism ÖZET Türk Toplumunda GSTM1, GSTP1 ve GSTT1 Gen Polimorfizmlerinin Prostat Kanseri Riski Üzerine EtkileriTürk toplumunda prostat kanseri riski üzerindeki etkilerini de¤erlendirmek amac› ile glutatyon S-transferaz M1, T1 ve P1 gen polimorfizmlerini bir vaka kontrol çal›flmas› ile araflt›rd›k. Polimorfizmler 115 kontrol ve 115 prostat kanseri hastas›nda polimeraz zincir reaksiyonuna dayal› yöntemler ile saptand›. Kontrol ve prostat kanseri gruplar›nda polimorfizmlerin s›kl›klar› lojistik regresyon analizi ile karfl›laflt›r›ld›. Ayr›ca genetik profiller ile Gleason skoru ve hasta yafllar› aras›nda bir iliflki varl›¤› araflt›r›ld›. GSTT1 null genotipin prostat kanseri riskini hafif derecede art›rd›¤› görülmekle birlikte (odds oran› (OR) = 1.50, %95 güven aral›¤› (CI), 0.85-2.66), sigara içenlerde prostat kanseri ile iliflkisinin istatistiksel olarak anlaml› oldu¤u saptand› (OR = 3.20, %95CI,). GSTP1 Val/Val genotipi vakalarda daha s›kt› (OR = 1.63, 95%CI, 0.61-4.37). Ancak, GSTP1 Ile/Ile ve GSTT1 null genotip kombinasyonunda risk anlaml› derecede daha yüksek olarak bulundu (OR = 2.67, %95CI, 1.00-7.10). Bu sonuçlar, Türk toplumunda GSTT1 null genotipin sigara içen bireylerde prostat kanseri riskini art›rd›¤›n› göstermifltir. Ayr›ca GSTT1 null ve GSTP1 Ile/Ile genotip birlikteli¤inin sigara kullan›m›ndan ba¤›ms›z olarak prostat kanseri riskini artt›rd›¤›n› göstermifltir.