Turkey ovarian tissue transplantation: effects of surgical technique on graft attachment and immunological status of the grafts, 6 days post-surgery

Hall, George B.; Long, Julie A.; Susta, Leonardo; Wood, Benjamin J.; Bédécarrats, Grégoy Y.

doi:10.1016/j.psj.2021.101648

Cited by 4 publications

(15 citation statements)

References 21 publications

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“…The procedure for ovarian transplantation in neonatal turkey poults followed the protocol described by Hall et al (12) with the following anesthetic modification. The anesthetic protocol was changed to IM administration of butorphanol (2 mg/kg), and xylazine (4 mg/kg) into the left pectoral muscle.…”

Section: Ovarian Transplantationmentioning

confidence: 99%

“…During surgery the entire recipient ovary was removed, and two pieces of ovarian tissue were transplanted into each recipient, but the abdominal air sac membrane was not used to cover the transplants (12). For the autotransplants in Study 1 the ovary was removed dissected into two pieces (2 × 2 mm 2 ), and then placed back into the same recipient poult.…”

Section: Ovarian Transplantationmentioning

confidence: 99%

“…Immunohistochemistry for cytoplasmic CD3 and nuclear PAX5 antigens were performed at the Animal Health Laboratory, University of Guelph (an American Association of Veterinary Laboratory Diagnosticians accredited laboratory), using an automated staining instrument (Dako Autostainer, Dako/Agilent, Ontario, Canada). The immunohistochemical reaction for CD3 followed the protocol detailed by Hall et al (12). For PAX5, after manual deparaffinization and rehydration, the sections were treated with 3% hydrogen peroxide to quench endogenous peroxidase activity.…”

Section: Immunohistochemistry For Cd3 and Pax5mentioning

confidence: 99%

“…Although biobanking of sperm has been optimized for some avian species (8,9), reliable cryopreservation and transplantation of ovarian tissue requires further optimization, especially in the domestic turkey. To date, ovarian transplant success rates in poultry breeds vary greatly between and within species and are technically challenging for routine use after biobanking (10)(11)(12). Thus, to ensure this technique can be used in the future to conserve poultry genetics, it is paramount to identify the factors that negatively influence transplant success rates in each species of interest, and to find remedies to counter those negative factors.…”

Section: Introductionmentioning

confidence: 99%

“…Our group has been focussing on possible reasons for ovarian transplant failure in the domestic turkey. In previous work, we elucidated the impact of age and ovarian developmental stage of the donor (13), along with the amount of recipient ovarian tissue which should remain to improve attachment rates (12). From these studies, we recommended that 7 days posthatch (dph) donor ovarian tissue is optimal as the ovary has few follicles, and that the entire recipient ovary should be removed, with the abdominal air sac membrane not used to cover the transplants.…”

Section: Introductionmentioning

confidence: 99%

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Cyclosporin A Prevents Ovarian Graft Rejection, and Permits Normal Germ Cell Maturation Within the First 5 Weeks Post-transplantation, in the Domestic Turkey (Meleagris gallopavo)

et al. 2022

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Biobanked ovaries collected from recently hatched poults can only be revived through transplantation, using a recipient bird. The main hurdle in transplantation is preventing graft rejection, which appears as lymphocytic infiltration upon histologic evaluation of the graft. In this study, the condition of the transplants [immunological compatibility (auto- vs. allotransplants), donor age, time in holding media, and temperature of holding media] and treatment of recipient poults with varying immunosuppressants [mycophenolate mofetil (MFM), cyclophosphamide (CY), and cyclosporin A (CsA)] were studied to determine which factors could reduce lymphocytic infiltration, during the first 35 days post-transplantation. Lymphocytic infiltration was determined via cytoplasmic CD3 (T cell) and nuclear PAX5 (B cell) expression. There was no significant difference in the percent of cytoplasmic CD3 or nuclear PAX5 immunostained area between the unoperated group and the autotransplants, by 6 days post-transplantation. However, the allotransplants had more (P < 0.05) positive cytoplasmic and nuclear immunostained areas compared to autotransplants, irrespective of donor age, time in holding media or temperature of the media. By 14 days post-transplantation, the CsA 25 and 50 mg/kg/day treatment groups had less (P < 0.05) CD3 and PAX5 positive areas in their allotransplants, compared to the unsuppressed group. At 35 days post-transplantation, the CsA 25 mg/kg/day allotransplant group also had less (P < 0.05) CD3 and PAX5 positive areas compared to the unsuppressed group. The CsA 25 mg/kg/day transplants also had a similar ovarian follicular size compared to the unoperated group, although they contained fewer (P < 0.05) follicles based on follicular density. Donor age, duration in holding media, temperature of media, and treatment of recipients with MFM or CY had no effect on reducing lymphocytic infiltration. However, immunological compatibility was associated with decreased lymphocytic infiltration, as autotransplants had little lymphocytic infiltration. Treatment of recipients with CsA at 25 mg/kg/day was also associated with reduced lymphocytic infiltration and allowed transplants to develop normally during the first 35 days post transplantation.

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Section: Ovarian Transplantationmentioning

confidence: 99%

Section: Ovarian Transplantationmentioning

confidence: 99%

Section: Immunohistochemistry For Cd3 and Pax5mentioning

confidence: 99%