Türkiye’de Kutanöz Leyşmanyazis Etkeni Leishmania tropica’da Antimon Direnç Mekanizmasının Belirlenmesi

Özbilgin, Ahmet; Zeyrek, Fadile Yıldız; Güray, Melda Zeynep; Çulha, Gülnaz; Akyar, Işın; Harman, Mehmet; Özbel, Yusuf; Ertabaklar, Hatice; Çavuş, İbrahim; Gündüz, Cumhur

doi:10.5578/mb.69702

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…Inversely, the Sb sensitivity was aligned with the downregulation of HSP83 in L. donovani (Kumar et al ., 2012). In addition, the CL caused by naturally Sb-resistant L. tropica was associated with HSP70 differential expression (Özbilgin et al ., 2021). The L. infantum promastigotes were superior to L. tarentolae in acquiring Sb(III) defence through robust HSP70 expression (Brochu et al ., 2004).…”

Section: Heat Shock Proteinsmentioning

confidence: 99%

Antimony resistance and gene expression inLeishmania: spotlight on molecular and proteomic aspects

Madusanka,

Karunaweera,

Silva

et al. 2023

Parasitology

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Leishmaniasis is a vector-borne parasitic disease caused by Leishmania parasites with a spectrum of clinical manifestations, ranging from skin lesions to severe visceral complications. Treatment of this infection has been extremely challenging with the concurrent emergence of drug resistance. The differential gene expression and the discrepancies in protein functions contribute to the appearance of 2 distinct phenotypes: resistant and sensitive, but the current diagnostic tools fail to differentiate between them. The identification of gene expression patterns and molecular mechanisms coupled with antimony (Sb) resistance can be leveraged to prompt diagnosis and select the most effective treatment methods. The present study attempts to use comparative expression of Sb resistance-associated genes in resistant and sensitive Leishmania, to disclose their relative abundance in clinical or in vitro selected isolates to gain an understanding of the molecular mechanisms of Sb response/resistance. Data suggest that the analysis of resistance gene expression would verify the Sb resistance or susceptibility only to a certain extent; however, none of the individual expression patterns of the studied genes was diagnostic as a biomarker of Sb response of Leishmania. The findings highlighted will be useful in bridging the knowledge gap and discovering innovative diagnostic tools and novel therapeutic targets.

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Section: Heat Shock Proteinsmentioning

confidence: 99%

Antimony resistance and gene expression inLeishmania: spotlight on molecular and proteomic aspects

Madusanka,

Karunaweera,

Silva

et al. 2023

Parasitology

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“…Serial dilutions were repeated three times on the same plate and at different times to be able to increase the reliability of the viability calculation. The absorbance was recorded at 450 nm after 4 h incubation at 37 • C. The viability percentages of the test compounds were calculated according to the following Equation [33,34]:…”

Section: Culture Of the Leishmania Tropica Isolatementioning

confidence: 99%

In Vitro and In Silico Evaluations of the Antileishmanial Activities of New Benzimidazole-Triazole Derivatives

Eser,

Çavuş

2023

Veterinary Sciences

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Benzimidazole and triazole rings are important pharmacophores, known to exhibit various pharmacological activities in drug discovery. In this study, it was purposed to synthesize new benzimidazole-triazole derivatives and evaluate their antileishmanial activities. The targeted compounds (5a–5h) were obtained after five chemical reaction steps. The structures of the compounds were confirmed by spectral data. The possible in vitro antileishmanial activities of the synthesized compounds were evaluated against the Leishmania tropica strain. Further, molecular docking and dynamics were performed to identify the probable mechanism of activity of the test compounds. The findings revealed that compounds 5a, 5d, 5e, 5f, and 5h inhibited the growth of Leishmania tropica to various extents and had significant anti-leishmanial activities, even if some orders were higher than the reference drug Amphotericin B. On the other hand, compounds 5b, 5c, and 5g were found to be ineffective. Additionally, the results of in silico studies have presented the existence of some interactions between the compounds and the active site of sterol 14-alpha-demethylase, a biosynthetic enzyme that plays a critical role in the growth of the parasite. Therefore, it can be suggested that if the results obtained from this study are confirmed with in vivo findings, it may be possible to obtain some new anti-leishmanial drug candidates.

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Investigating the Effect of Satureja khuzestanica Essential oil on MDR1 Gene Expression in Leishmania major

Jahanshahi,

Kheirandish,

Kazemi

et al. 2024

Acta Parasit.

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Türkiye’de Kutanöz Leyşmanyazis Etkeni Leishmania tropica’da Antimon Direnç Mekanizmasının Belirlenmesi

Cited by 7 publications

References 20 publications

Antimony resistance and gene expression inLeishmania: spotlight on molecular and proteomic aspects

Antimony resistance and gene expression inLeishmania: spotlight on molecular and proteomic aspects

In Vitro and In Silico Evaluations of the Antileishmanial Activities of New Benzimidazole-Triazole Derivatives

Investigating the Effect of Satureja khuzestanica Essential oil on MDR1 Gene Expression in Leishmania major

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