Türkiye’de Sığırlardan İzole Edilen M. tuberculosis Suşlarının Spoligotiplendirmesi

Abstract

Although it is generally accepted that M.bovis leads to tuberculosis in cattle, there are statements given from the different regions of the world, referring to the fact that M. tuberculosis, which is known as the human tuberculosis agent, causes tuberculosis in cattle as well. The material of the study consisted of 13 M. tuberculosis isolates which were isolated and identified from the organ pieces of 95 cattle with the culture methods; these organ pieces had been taken from the cattle with granulomatous lesion detection after the slaughtering in slaughterhouses located in Çukurova region and brought to the laboratory under sterile conditions. It was determined in the genotyping conducted by using the Spoligotyping method that 13 of the 55 isolates were M. tuberculosis and they belonged to the T1 family (SIT53) by becoming dense in one cluster (100%). Consequently, it was shown with this study that M. tuberculosis, which leads to tuberculosis in humans, could be transmitted from humans to animals and from animals to humans again, and researching the human and epidemiological tuberculosis cases by using molecular epidemiology-based methods such as spoligotyping might provide useful information about explaining the ways of transmission of tuberculosis. Türkiye'de Sığırlardan İzole Edilen M. tuberculosis Suşlarının Spoligotiplendirmesi Özet Tüberkülozise sığırlarda, M. bovis'in sebep olduğu genel olarak kabul edilmesine rağmen insan tüberkülozis etkeni olarak bilinen M. tuberculosis'in de sığırlarda tüberkülozise neden olduğuna dair dünyanın farklı bölgelerinden yapılmış bildirimler bulunmaktadır. Çalışmanın materyalini Çukurova bölgesinde bulunan mezbahalarda kesim sonrası granülamatöz lezyon tespit edilen sığırlardan alınan ve steril şartlarda laboratuvara ulaştırılan 95 adet sığıra ait organ parçalarından kültür yöntemleri ile izole ve identifiye edilen 13 adet M. tuberculosis izolatı oluşturdu. Spoligotyping yöntemi ile yapılan genotiplendirmede 55 izolattan 13 tanesinin M. tuberculosis olduğu ve bunların tek bir küme içerisinde yoğunlaşarak (%100) T1 ailesine (SIT53) ait olduğu belirlendi. Sonuç olarak bu çalışma ile insanlarda tüberkülozise neden olan M. tuberculosis'in insanlardan hayvanlara, hayvanlardan da tekrar insanlara bulaşabileceği, insan ve hayvan kaynaklı tüberkülozis vakalarının spoligotyping gibi moleküler epidemiyolojik temelli yöntemlerle araştırılmasının tüberkülozisin bulaş yollarının açıklanması konusunda faydalı bilgiler verebileceği gösterilmiştir.

New spoligotyping pattern of Mycobacterium bovis isolates from farm animals in Turkey

“…Tuzcu et al (20), in a report on M. bovis strains isolated from cattle slaughtered in Çukurova region, have found that 11 of the strains were M. bovis SIT 683 (SB0140), 4 of them were ST 685 (SB288), 7 were M. bovis subsp. caprae ST 647 (SB0418) while 2 strains' spoligotype pattern (220003777377600, 674077777777600) did not match any of the strains in the database.…”

Genetic evaluation of Mycobacteriumbovis isolates with MIRU-VNTR and spoligotyping

“…Spoligotyping was carried out according to the manufacturer's instructions (Spoligotyping Kit; Isogen LifeScience, Utrecht, The Netherlands) and described by Kamerbeek et al after DNA isolation from 42 M. bovis samples [22][23][24]. In order to obtain DNA from spoligotyping and MIRU-VNTR applied to all 42 M. bovis isolates, extraction was made from 7H9 broth medium and LJ medium by using a Mickle tissue disintegrator.…”

Isolation and analysis of the molecular epidemiology and zoonotic significance of Mycobacterium tuberculosis in domestic and wildlife ruminants from three states in India

“…Republic of China, Nigeria and Spain (6,7,8,9,10), and also, in India (11,12). In addition, in the district of Gandhinagar, Gujarat state, in western India, captive wild ruminants infected with MT have been reported (13).…”