Turmeric (Curcuma longa) Rhizome Extract and Selenium Protect Effects of Nerium indicum Leaf Extract on Serum Calcium and Phosphate of Male Wistar Rats

Abstract

Male Wistar rats were divided into 6 groups (A-F) and treated as --Group A: Control; Group B: Nerium indicum leaves extract (NLE; 25 mg/kg b wt); Group C: Nerium indicum leaves extract (NLE; 25 mg /kg b wt) + selenium extract (Se; 0.5 mg/ kg b wt); Group D: Nerium indicum leaves extract (NLE; 25 mg/ kg b wt) + Curcuma longa rhizome extract (CRE; 200 mg/kg b wt); Group E: Selenium extract (Se; 0.5 mg/ kg b wt); Group F: Curcuma longa rhizome extract (CRE; 200 mg/kg b wt). Serum calcium and phosphate levels were analyzed on 15 and 30 days after exposure of above mentioned treatments.Rats from group B showed a progressive decrease in serum calcium level as compared to group A (control) from day 15 to day 30. In group C and group D the serum calcium levels showed a decrease on day 15 as compared to the control (group A). On day 30, the levels in group C and group D were increased as compared to group B but it is still hypocalcemic as compared to control. This shows that the serum calcium levels were slightly recovered as compared to group B after administration of selenium and Curcuma longa rhizome extract after 30 days. There is no alteration in serum calcium levels of group E and F as compared to group A.Exposure of Nerium indicum leaves extract (group B) to rats showed a decrease in serum phosphate level at days 15 and 30 as compared to control (group A). There is increase in serum phosphate levels of group C and group D on day 15 and 30 as compared to group B. This clearly indicated that the phosphate levels which were decreased by exposure to Nerium indicum leaves extract recovered after administration of selenium and Curcuma longa rhizome extract. No alteration was noticed in serum phosphate levels of group E and group F.