“Turn off–on” phosphorescent biosensors for detection of DNA based on quantum dots/acridine orange

Miao, Yanming; Li, Yuting; Zhang, Zhifeng; Yan, Guiqin; Bi, Yi

doi:10.1016/j.ab.2015.01.011

Cited by 30 publications

(7 citation statements)

References 55 publications

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“…Scheme 1), is a well-known DNA intercalator. 1,4,15,16 In dilute aqueous solution the dye mainly exists in its monomeric form. Above about 10 mM concentration, however, the dye undergoes a reasonable extent of dimer formation, and above about 1 mM concentration the dye almost exclusively exists in the dimeric form.…”

Section: Introductionmentioning

confidence: 99%

Unraveling multiple binding modes of acridine orange to DNA using a multispectroscopic approach

Sayed

Krishnamurthy

Pal

2016

Phys. Chem. Chem. Phys.

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The interaction of acridine orange (AOH(+)) with calf thymus DNA (ct-DNA) under different dye-DNA conditions has been investigated in detail using multispectroscopic techniques, unraveling a number of hitherto unexplored intricacies of dye-DNA binding. The observed results intriguingly show contrasting binding features when low (2.4 μM) and significantly high (23 μM) dye concentrations are used. It is conclusively inferred from absorption, steady-state fluorescence, circular dichroism, fluorescence decay and anisotropy decay studies that at low [DNA] to [dye] ratio, especially with higher dye concentration, dimeric AOH(+) predominantly binds externally to DNA surfaces through electrostatic interactions. At sufficiently high [DNA] to [dye] ratios, however, the interaction intriguingly changes to monomeric AOH(+) bound to DNA, predominantly in the intercalative mode between DNA base pairs, with partly an electrostatic binding on DNA surfaces. With very low initial dye concentration, monomeric (AOH(+)) mostly binds to DNA through intercalative and electrostatic modes for most DNA to dye ratios. The present study demonstrates a systematic correlation of the striking changes in the photophysical properties of the dye upon multimode binding with DNA. The observed results are of great significance in understanding the fundamental insights of dye/drug binding to DNA hosts, of use in the design of effective therapeutic agents.

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Section: Introductionmentioning

confidence: 99%

Unraveling multiple binding modes of acridine orange to DNA using a multispectroscopic approach

Sayed

Krishnamurthy

Pal

2016

Phys. Chem. Chem. Phys.

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“…Here, we use this technique together with a well-known DNA and RNA intercalator, acridine orange (AO-see Figure S8 ). 41 Concentrations of AO around 0.3 mg/mL (1 mM) or higher lead to the formation of nonfluorescent dimers. 42 However, AO can intercalate in nucleic acid helices as the cationic monomer can presumably bind through electrostatic interaction with negatively charged phosphate.…”

Section: Resultsmentioning

confidence: 99%

Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility

Rocca²,

Villarmois³

et al. 2018

ACS Omega

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Photoinduced antibacterial gold nanoparticles were developed as an alternative for the treatment of antibiotic-resistant bacteria. Thanks to the amoxicillin coating, they possess high in vivo stability, selectivity for the bacteria wall, a good renal clearance, and are completely nontoxic for eukaryotic cells at the bactericidal concentrations. A simple one-step synthesis of amoxi@AuNP is described at mild temperatures using the antibiotic as both reducing and stabilizing agent. Time-resolved fluorescence microscopy proved these novel nano-photosensitizers, with improved selectivity, are bactericidal but showing excellent biocompatibility toward eukaryotic cells at the same dose (1.5 μg/mL) when co-cultures are analyzed. Their stability in biological media, hemocompatibility, and photo-antibacterial effect against sensitive and antibiotic-resistant Staphylococcus aureus were evaluated in vitro, whereas toxicity, renal clearance, and biodistribution were studied in vivo in male Wistar rats. The use of these nanoparticles to treat antibiotic-resistant infections is promising given their high stability and cytocompatibility.

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“…Mn-ZnS QDs were prepared as reported before. [29][30][31] First, a 250 mL three-necked bottles was added with 10 mL of 0.1 mol L À1 Zn(Ac) 2 , 4 mL of 0.01 mol L À1 Mn(Ac) 2 and 100 mL of 0.04 mol L À1 MPA. The mixture was adjusted to pH 11 by adding a 1 mol L À1 NaOH solution, and then magnetically stirred for 30 min at room temperature and argon ventilation.…”

Section: Synthesis Of Mn-zns Qdsmentioning

confidence: 99%

Detection of tumor marker miRNA21 based on phosphorescent resonance energy transfer of Mn–ZnS QDs

Miao

Yan

2017

RSC Adv.

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“Turn off–on” phosphorescent biosensors for detection of DNA based on quantum dots/acridine orange

Cited by 30 publications

References 55 publications

Unraveling multiple binding modes of acridine orange to DNA using a multispectroscopic approach

Unraveling multiple binding modes of acridine orange to DNA using a multispectroscopic approach

Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility

Detection of tumor marker miRNA21 based on phosphorescent resonance energy transfer of Mn–ZnS QDs

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