Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform

Liu, Jinshui; Dong, Zhenzhen; Yang, Chao; Li, Guodong; Wu, Cheng‐Shyong; Lee, Fu-Wa; Leung, Chung‐Hang; Ma, Dik‐Lung

doi:10.1038/s41598-017-09478-6

Cited by 25 publications

(6 citation statements)

References 49 publications

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“…Hydrogen peroxide (H 2 O 2 ), as the most representative of redox oxygen species studied in cellular environments, not only is involved in immune response, oxidative stress, and pathogen invasion but also performs as secondary messengers, playing important roles in essential cellular processes. , Therefore, detection of H 2 O 2 released from living cells is essential to fully understand its roles in cellular physiology, as well as providing reliable diagnosis of pathological conditions. Seminal contributions have demonstrated that numerous analytical approaches, for example, fluorescence, − chemiluminescence, electrochemiluminescence, and electrochemical − methods, can be utilized for fast and accurate detection of H 2 O 2 released from living cells. Generally, special and effective signal molecules (fluorescence probes, etc.)…”

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confidence: 99%

Organic Electrochemical Transistor for in Situ Detection of H₂O₂ Released from Adherent Cells and Its Application in Evaluating the In Vitro Cytotoxicity of Nanomaterial

et al. 2019

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Detection of hydrogen peroxide (H2O2) produced by living cells is very significant to fully understand its roles in cellular physiology, as well as providing reliable diagnosis of pathological conditions. However, in situ detection of H2O2 released from adherent cells in cellular culture medium is still insufficiently achieved. Here, we report an electrochemical platform for in situ detection of H2O2 produced by adherent cells in cellular culture medium. It is based on the use of organic electrochemical transistor (OECT) fabricated on a flexible poly(ethylene terephthalate) substrate and Transwell support. A screen-printed carbon paste electrode was modified with carbon nanotubes and platinum nanoparticles and served as the gate of the device. Under optimal conditions, this device exhibits good modulation and sensitivity. It works in the 0.5 μM to 0.1 mM H2O2 concentration range and has a 0.2 μM detection limit. The cells were seeded and grew on the Transwell membrane. Upon being stimulated by N-formylmethionyl-leucyl-phenylalanine peptide, H2O2 produced by the adherent cells diffused into the bottom chamber of the Transwell and was in situ detected by OECT. Moreover, evaluating in vitro cytotoxicity of the nanomaterial using the OECT-Transwell platform was realized. This simple electrochemical platform would be of great interest for in vitro cytotoxicity, cellular physiology study, and diagnosis of pathological conditions.

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confidence: 99%

Organic Electrochemical Transistor for in Situ Detection of H₂O₂ Released from Adherent Cells and Its Application in Evaluating the In Vitro Cytotoxicity of Nanomaterial

et al. 2019

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“…817 Ma and co-workers have developed a nanosensor for sensitive detection of H 2 O 2 . 818 The nanosensor utilizes AgNPs as a responsive unit and an iridium(III) complex (431) (Chart 193) as a signaling unit. The emission of the complex is effectively quenched upon adsorption onto the AgNPs due to efficient energy transfer.…”

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confidence: 99%

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Lee,

2024

Chem. Rev.

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Luminescence imaging is a powerful and versatile technique for investigating cell physiology and pathology in living systems, making significant contributions to life science research and clinical diagnosis. In recent years, luminescent transition metal complexes have gained significant attention for diagnostic and therapeutic applications due to their unique photophysical and photochemical properties. In this Review, we provide a comprehensive overview of the recent development of luminescent transition metal complexes for bioimaging and biosensing applications, with a focus on transition metal centers with a d 6 , d 8 , and d 10 electronic configuration. We elucidate the structure−property relationships of luminescent transition metal complexes, exploring how their structural characteristics can be manipulated to control their biological behavior such as cellular uptake, localization, biocompatibility, pharmacokinetics, and biodistribution. Furthermore, we introduce the various design strategies that leverage the interesting photophysical properties of luminescent transition metal complexes for a wide variety of biological applications, including autofluorescence-free imaging, multimodal imaging, organelle imaging, biological sensing, microenvironment monitoring, bioorthogonal labeling, bacterial imaging, and cell viability assessment. Finally, we provide insights into the challenges and perspectives of luminescent transition metal complexes for bioimaging and biosensing applications, as well as their use in disease diagnosis and treatment evaluation.

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“…Understanding the function and regulatory mechanisms of metalloproteinases may provide insights into the future development of diagnostic and therapeutic approaches (e.g. nanomaterials or metal-based probe targeting these enzymes) for chronic inflammatory diseases ( 20 – 24 ).…”

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confidence: 99%

Editorial: Immunomodulatory role of metalloproteases in chronic inflammatory diseases

Yang

et al. 2023

Front. Immunol.

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Editorial on the Research TopicImmunomodulatory role of metalloproteases in chronic inflammatory diseases Metalloproteases are a diverse class of enzymes involved in the regulation of numerous pathological and physiological processes. Evidence has shown that metalloproteases could either directly or indirectly regulate the secretion of chemokines and the differentiation and/or activation of immune cells, thereby mediating many inflammatory and innate immune responses (1, 2). While different metalloproteases could have substantially different primary structure, their active center which contains metal ions (e.g. iron, zinc, cobalt, nickel ions) is relatively conservative. Metalloprotease relies on metal ions to maintain its catalytic function. Studies have shown that metal chelators such as EDTA could completely inactivate metalloproteases (3-6; Liu et al.). Under inflammatory conditions, metalloproteases are constitutively activated or deactivated in multiple immune-or non-immune cells and could contribute to a variety of inflammatory diseases, such as rheumatoid arthritis (RA) (Li et al.), chronic enteritis (Deng et al. and Mei et al.), allergic dieseases (Wang and Wang and Bendavid et al.), diabetes (Chen et al.), and cancers (He et al.), etc. Mutiple chronic inflammatory diseases could even hijack various metalloproteases to promote and exacerbate inflammation (1, 2). Numerous preclinical and clinical studies have shown that metalloprotease modulators, including lysine-specific demethyalses (KDMs) inhibitors (3-5, 7), histone deacetylases (HDACs) inhibitors (8), and matrix metalloproteinases (MMPs) inhibitors (6), and a disintegrin and metalloproteinases (ADAMs) inhibitors (9), possess in vitro and in vivo anti-inflammatory activities. Therefore, understanding the roles of metalloproteinases in the immune system may potenitally uncover new targets for the diagnosis and treatment of chronic inflammatory diseases.

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Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform

Cited by 25 publications

References 49 publications

Organic Electrochemical Transistor for in Situ Detection of H₂O₂ Released from Adherent Cells and Its Application in Evaluating the In Vitro Cytotoxicity of Nanomaterial

Organic Electrochemical Transistor for in Situ Detection of H₂O₂ Released from Adherent Cells and Its Application in Evaluating the In Vitro Cytotoxicity of Nanomaterial

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Editorial: Immunomodulatory role of metalloproteases in chronic inflammatory diseases

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Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex–Silver Nanoparticle Platform

Cited by 25 publications

References 49 publications

Organic Electrochemical Transistor for in Situ Detection of H2O2 Released from Adherent Cells and Its Application in Evaluating the In Vitro Cytotoxicity of Nanomaterial

Organic Electrochemical Transistor for in Situ Detection of H2O2 Released from Adherent Cells and Its Application in Evaluating the In Vitro Cytotoxicity of Nanomaterial

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Editorial: Immunomodulatory role of metalloproteases in chronic inflammatory diseases

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Product

Resources

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Organic Electrochemical Transistor for in Situ Detection of H₂O₂ Released from Adherent Cells and Its Application in Evaluating the In Vitro Cytotoxicity of Nanomaterial

Organic Electrochemical Transistor for in Situ Detection of H₂O₂ Released from Adherent Cells and Its Application in Evaluating the In Vitro Cytotoxicity of Nanomaterial